NUP54_DROME
ID NUP54_DROME Reviewed; 610 AA.
AC Q9V6B9;
DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Probable nucleoporin Nup54;
GN Name=Nup54; ORFNames=CG8831;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, IDENTIFICATION IN THE NUCLEAR PORE COMPLEX, AND SUBCELLULAR
RP LOCATION.
RX PubMed=17682050; DOI=10.1083/jcb.200612135;
RA Sabri N., Roth P., Xylourgidis N., Sadeghifar F., Adler J., Samakovlis C.;
RT "Distinct functions of the Drosophila Nup153 and Nup214 FG domains in
RT nuclear protein transport.";
RL J. Cell Biol. 178:557-565(2007).
RN [5]
RP FUNCTION, AND INTERACTION WITH NUP58; FS(1)YB; SBR AND NXT1.
RX PubMed=33856346; DOI=10.7554/elife.66321;
RA Munafo M., Lawless V.R., Passera A., MacMillan S., Borneloev S.,
RA Haussmann I.U., Soller M., Hannon G.J., Czech B.;
RT "Channel Nuclear Pore Complex subunits are required for transposon
RT silencing in Drosophila.";
RL Elife 10:0-0(2021).
CC -!- FUNCTION: Component of the nuclear pore complex (NPC), a complex
CC required for the trafficking across the nuclear envelope
CC (PubMed:17682050). Essential for the nuclear import of nuclear
CC localization signal (NLS)-containing proteins in an importin
CC alpha/importin beta-dependent manner (PubMed:17682050). Together with
CC Nup58, required for transposable element silencing regulation in
CC ovarian follicle cells (PubMed:33856346). By interacting with the
CC nuclear (Nxf1/Nxt1) and cytosolic (fs(1)Yb) components of the flamenco
CC (flam) transcripts processing pathway, enables export and subsequent
CC piRNA production (PubMed:33856346). {ECO:0000269|PubMed:17682050,
CC ECO:0000269|PubMed:33856346}.
CC -!- SUBUNIT: Component of the nuclear pore complex (PubMed:17682050).
CC Interacts with Nup58 (PubMed:33856346). Interacts (via C-terminus) with
CC fs(1)Yb; this interaction occurs in a RNA-independent manner
CC (PubMed:33856346). Interacts with sbr/nxf1 (PubMed:33856346). Interacts
CC with Nxt1 (PubMed:33856346). {ECO:0000269|PubMed:17682050,
CC ECO:0000269|PubMed:33856346}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000269|PubMed:17682050}.
CC -!- DOMAIN: Contains FG repeats. FG repeats are interaction sites for
CC karyopherins (importins, exportins) and form probably an affinity
CC gradient, guiding the transport proteins unidirectionally with their
CC cargo through the NPC. FG repeat regions are highly flexible and lack
CC ordered secondary structure. The overall conservation of FG repeats
CC regarding exact sequence, spacing, and repeat unit length is limited.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NUP54 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF58509.1; -; Genomic_DNA.
DR EMBL; AY051713; AAK93137.1; -; mRNA.
DR RefSeq; NP_610783.1; NM_136939.4.
DR AlphaFoldDB; Q9V6B9; -.
DR SMR; Q9V6B9; -.
DR BioGRID; 62137; 32.
DR DIP; DIP-19285N; -.
DR IntAct; Q9V6B9; 16.
DR MINT; Q9V6B9; -.
DR STRING; 7227.FBpp0087063; -.
DR PaxDb; Q9V6B9; -.
DR PRIDE; Q9V6B9; -.
DR DNASU; 36360; -.
DR EnsemblMetazoa; FBtr0087952; FBpp0087063; FBgn0033737.
DR GeneID; 36360; -.
DR KEGG; dme:Dmel_CG8831; -.
DR UCSC; CG8831-RA; d. melanogaster.
DR CTD; 53371; -.
DR FlyBase; FBgn0033737; Nup54.
DR VEuPathDB; VectorBase:FBgn0033737; -.
DR eggNOG; KOG3091; Eukaryota.
DR GeneTree; ENSGT00390000013620; -.
DR HOGENOM; CLU_033371_0_0_1; -.
DR InParanoid; Q9V6B9; -.
DR OMA; VYNKVNE; -.
DR OrthoDB; 559281at2759; -.
DR PhylomeDB; Q9V6B9; -.
DR Reactome; R-DME-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-DME-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-DME-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-DME-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-DME-3108214; SUMOylation of DNA damage response and repair proteins.
DR Reactome; R-DME-3301854; Nuclear Pore Complex (NPC) Disassembly.
DR Reactome; R-DME-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-DME-4085377; SUMOylation of SUMOylation proteins.
DR Reactome; R-DME-4551638; SUMOylation of chromatin organization proteins.
DR Reactome; R-DME-4615885; SUMOylation of DNA replication proteins.
DR Reactome; R-DME-5578749; Transcriptional regulation by small RNAs.
DR SignaLink; Q9V6B9; -.
DR BioGRID-ORCS; 36360; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36360; -.
DR PRO; PR:Q9V6B9; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033737; Expressed in egg cell and 40 other tissues.
DR ExpressionAtlas; Q9V6B9; baseline and differential.
DR Genevisible; Q9V6B9; DM.
DR GO; GO:0005643; C:nuclear pore; ISS:UniProtKB.
DR GO; GO:0044613; C:nuclear pore central transport channel; IBA:GO_Central.
DR GO; GO:0017056; F:structural constituent of nuclear pore; IMP:FlyBase.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IMP:FlyBase.
DR GO; GO:0006999; P:nuclear pore organization; IBA:GO_Central.
DR GO; GO:0006913; P:nucleocytoplasmic transport; ISS:UniProtKB.
DR GO; GO:0006606; P:protein import into nucleus; IMP:FlyBase.
DR GO; GO:0036228; P:protein localization to nuclear inner membrane; IBA:GO_Central.
DR GO; GO:0006605; P:protein targeting; ISS:UniProtKB.
DR InterPro; IPR024864; Nup54/Nup57/Nup44.
DR InterPro; IPR025712; Nup54_alpha-helical_dom.
DR InterPro; IPR040985; Nup54_C.
DR PANTHER; PTHR13000; PTHR13000; 1.
DR Pfam; PF13874; Nup54; 1.
DR Pfam; PF18437; Nup54_C; 1.
PE 1: Evidence at protein level;
KW mRNA transport; Nuclear pore complex; Nucleus; Protein transport;
KW Reference proteome; Repeat; Translocation; Transport.
FT CHAIN 1..610
FT /note="Probable nucleoporin Nup54"
FT /id="PRO_0000204873"
FT REPEAT 4..5
FT /note="1"
FT REPEAT 24..25
FT /note="2"
FT REPEAT 28..29
FT /note="3"
FT REPEAT 41..42
FT /note="4"
FT REPEAT 51..52
FT /note="5"
FT REPEAT 60..61
FT /note="6"
FT REPEAT 66..67
FT /note="7"
FT REPEAT 75..76
FT /note="8"
FT REPEAT 84..85
FT /note="9"
FT REPEAT 89..90
FT /note="10"
FT REPEAT 95..96
FT /note="11"
FT REPEAT 112..113
FT /note="12"
FT REPEAT 115..116
FT /note="13"
FT REPEAT 127..128
FT /note="14"
FT REPEAT 142..143
FT /note="15"
FT REPEAT 159..160
FT /note="16"
FT REPEAT 169..170
FT /note="17"
FT REPEAT 172..173
FT /note="18"
FT REPEAT 180..181
FT /note="19"
FT REPEAT 185..186
FT /note="20"
FT REPEAT 189..190
FT /note="21"
FT REPEAT 201..202
FT /note="22"
FT REPEAT 204..205
FT /note="23"
FT REPEAT 237..238
FT /note="24"
FT REPEAT 324..325
FT /note="25"
FT REGION 4..554
FT /note="25 X 2 AA repeats of F-G"
SQ SEQUENCE 610 AA; 64397 MW; 77D139079E6B4C01 CRC64;
MSFFGSNTSL GATSTPAKTT GGLFGSPFGG TAATSQPAPA FGAQATSTPA FGAQPATSAF
GAGSAFGATA AAPAFGAATG TSAFGGSAFG STPAFGAATT TTAGTGLGGG GFGGFGAAPA
TSQAGLFGAP ATSAAPPAFS GFGQQAAAST APASGFSGFG TTTTSAPAFG GFGTSQSTGF
GGGAFGSTFG KPANTTVTPG FGGFGGTSFM LGQPQQQPAP ISADEAFAQS ILNVSIFGDE
RDKIVAKWNY LQATWGTGKM FYSQSAAPVD ITPENVMCRF KAIGYSRMPG KDNKLGLVAL
NFCRELSAVK PHQQQVIQTL HSLFGSKPNM LVHIDSIKEL ENKKCQIVIY VEEKLQHAPN
ESKRILATEL SNYLNQATLK PQLNNLGVVE ALALVLPDED QLREYLENPP RGVDPRMWRQ
ANSDNPDPTL YIPVPMVGFN DLKWRVKCQE QETDTHALYI KKVESELTEL KKRHATATAK
ILEHKRKLAE LSHRILRIIV KQECTRKVGT SLTPEEEALR TKLQNMLAVV SAPTQFKGRL
SELLSQMRMQ RNQFAANGGA EYALDKEAED EMKTFLTMQQ RAMEVLSDTV NKDLRALDVI
IKGLPELRQS
