NUP54_MOUSE
ID NUP54_MOUSE Reviewed; 510 AA.
AC Q8BTS4; Q3TSI2; Q8BU42;
DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Nuclear pore complex protein Nup54;
DE AltName: Full=54 kDa nucleoporin;
DE AltName: Full=Nucleoporin Nup54;
GN Name=Nup54;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Muellerian duct, Ovary, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 144-155.
RC TISSUE=Brain;
RA Lubec G., Yang J.W., Zigmond M.;
RL Submitted (JUL-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the nuclear pore complex, a complex required for
CC the trafficking across the nuclear membrane.
CC {ECO:0000250|UniProtKB:P70582}.
CC -!- SUBUNIT: Component of the p62 complex, a complex composed of NUP62,
CC NUP54, and the isoform p58 and isoform p45 of NUP58. Interacts with
CC NUTF2. {ECO:0000250|UniProtKB:P70582}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000250|UniProtKB:P70582}. Nucleus membrane
CC {ECO:0000250|UniProtKB:P70582}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P70582}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:P70582}. Nucleus membrane
CC {ECO:0000250|UniProtKB:P70582}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P70582}; Nucleoplasmic side
CC {ECO:0000250|UniProtKB:P70582}. Note=Biased towards cytoplasmic side.
CC Central region of the nuclear pore complex, within the transporter.
CC {ECO:0000250|UniProtKB:P70582}.
CC -!- DOMAIN: Contains FG repeats.
CC -!- PTM: O-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NUP54 family. {ECO:0000305}.
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DR EMBL; AK087827; BAC40018.1; -; mRNA.
DR EMBL; AK088855; BAC40615.1; -; mRNA.
DR EMBL; AK133841; BAE21878.1; -; mRNA.
DR EMBL; AK162037; BAE36693.1; -; mRNA.
DR EMBL; BC068114; AAH68114.1; -; mRNA.
DR CCDS; CCDS19430.1; -.
DR RefSeq; NP_899248.1; NM_183392.2.
DR AlphaFoldDB; Q8BTS4; -.
DR SMR; Q8BTS4; -.
DR ComplexPortal; CPX-4474; Nuclear pore complex.
DR STRING; 10090.ENSMUSP00000046540; -.
DR iPTMnet; Q8BTS4; -.
DR PhosphoSitePlus; Q8BTS4; -.
DR REPRODUCTION-2DPAGE; Q8BTS4; -.
DR EPD; Q8BTS4; -.
DR MaxQB; Q8BTS4; -.
DR PaxDb; Q8BTS4; -.
DR PeptideAtlas; Q8BTS4; -.
DR PRIDE; Q8BTS4; -.
DR ProteomicsDB; 293907; -.
DR Antibodypedia; 24771; 109 antibodies from 23 providers.
DR DNASU; 269113; -.
DR Ensembl; ENSMUST00000038514; ENSMUSP00000046540; ENSMUSG00000034826.
DR GeneID; 269113; -.
DR KEGG; mmu:269113; -.
DR UCSC; uc008ydk.1; mouse.
DR CTD; 53371; -.
DR MGI; MGI:1920460; Nup54.
DR VEuPathDB; HostDB:ENSMUSG00000034826; -.
DR eggNOG; KOG3091; Eukaryota.
DR GeneTree; ENSGT00390000013620; -.
DR HOGENOM; CLU_033371_0_0_1; -.
DR InParanoid; Q8BTS4; -.
DR OMA; VYNKVNE; -.
DR OrthoDB; 1506658at2759; -.
DR PhylomeDB; Q8BTS4; -.
DR TreeFam; TF320237; -.
DR Reactome; R-MMU-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-MMU-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-MMU-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-MMU-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-MMU-170822; Regulation of Glucokinase by Glucokinase Regulatory Protein.
DR Reactome; R-MMU-191859; snRNP Assembly.
DR Reactome; R-MMU-3108214; SUMOylation of DNA damage response and repair proteins.
DR Reactome; R-MMU-3232142; SUMOylation of ubiquitinylation proteins.
DR Reactome; R-MMU-3301854; Nuclear Pore Complex (NPC) Disassembly.
DR Reactome; R-MMU-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-MMU-4085377; SUMOylation of SUMOylation proteins.
DR Reactome; R-MMU-4551638; SUMOylation of chromatin organization proteins.
DR Reactome; R-MMU-4570464; SUMOylation of RNA binding proteins.
DR Reactome; R-MMU-4615885; SUMOylation of DNA replication proteins.
DR Reactome; R-MMU-5578749; Transcriptional regulation by small RNAs.
DR BioGRID-ORCS; 269113; 18 hits in 72 CRISPR screens.
DR ChiTaRS; Nup54; mouse.
DR PRO; PR:Q8BTS4; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8BTS4; protein.
DR Bgee; ENSMUSG00000034826; Expressed in epiblast (generic) and 229 other tissues.
DR ExpressionAtlas; Q8BTS4; baseline and differential.
DR Genevisible; Q8BTS4; MM.
DR GO; GO:0005635; C:nuclear envelope; ISO:MGI.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005643; C:nuclear pore; ISO:MGI.
DR GO; GO:0044613; C:nuclear pore central transport channel; IBA:GO_Central.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR GO; GO:0017056; F:structural constituent of nuclear pore; ISO:MGI.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IBA:GO_Central.
DR GO; GO:0006999; P:nuclear pore organization; IBA:GO_Central.
DR GO; GO:0006913; P:nucleocytoplasmic transport; ISO:MGI.
DR GO; GO:0036228; P:protein localization to nuclear inner membrane; IBA:GO_Central.
DR GO; GO:0006605; P:protein targeting; ISO:MGI.
DR GO; GO:0042306; P:regulation of protein import into nucleus; ISO:MGI.
DR InterPro; IPR024864; Nup54/Nup57/Nup44.
DR InterPro; IPR025712; Nup54_alpha-helical_dom.
DR InterPro; IPR040985; Nup54_C.
DR PANTHER; PTHR13000; PTHR13000; 1.
DR Pfam; PF13874; Nup54; 1.
DR Pfam; PF18437; Nup54_C; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Membrane; mRNA transport;
KW Nuclear pore complex; Nucleus; Protein transport; Reference proteome;
KW Repeat; Translocation; Transport.
FT CHAIN 1..510
FT /note="Nuclear pore complex protein Nup54"
FT /id="PRO_0000204875"
FT REPEAT 5..6
FT /note="1"
FT REPEAT 25..26
FT /note="2"
FT REPEAT 28..29
FT /note="3"
FT REPEAT 61..62
FT /note="4"
FT REPEAT 63..64
FT /note="5"
FT REPEAT 67..68
FT /note="6"
FT REPEAT 87..88
FT /note="7"
FT REPEAT 447..448
FT /note="8"
FT REGION 5..448
FT /note="8 X 2 AA repeats of F-G"
SQ SEQUENCE 510 AA; 55732 MW; FD70E4574964BE30 CRC64;
MAFNFGAPSG TSGTSTATAA PAGGFGGFGT TTTTAGSAFS FSAPTNTGST GLLGGTQNKG
FGFGTGFGTT TGTGTGLGTG LGTGLGFGGF NTQQQQQQQQ TSLGGLFSQP TQAPAQSTQL
INTASALSAP TLLGDERDAI LAKWNQLQAF WGTGKGYFNN NIPPVEFTQE NPFCRFKAVG
YSCMPNNKDE DGLVVLIFNK KETDIRSQQQ QLVESLHKVL GGNQTLTVNV EGIKTLPDDQ
TEVVIYVVER SPNGTSRRVP ATTLYAHFEQ ANIKAQLQQL GVTLSMTRTE LSPAQIKQLL
QNPPAGVDPI IWEQAKVDNP DSEKLIPVPM VGFKELLRRL KVQDQMTKQH QTRLDIISED
ISELQKNQTT TMAKIAQYKR KLMELSHRTL QVLIKQEIQR KSGYAIQADE EQLRVQLDTI
QGELNAPTQF KGRLNELMSQ IRMQNHFGAV KSEEKYYIDA DLLREIKQHL KQQQEGLSHL
ISIIKDDLED IKLVEHGLNE TIHSRGGVFS
