NUP54_RAT
ID NUP54_RAT Reviewed; 510 AA.
AC P70582;
DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Nuclear pore complex protein Nup54;
DE AltName: Full=54 kDa nucleoporin;
DE AltName: Full=Nucleoporin Nup54;
GN Name=Nup54;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 219-234; 298-316; 354-365;
RP 434-442 AND 456-464, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN A
RP COMPLEX WITH NUP62 AND NUP58, AND INTERACTION WITH NUTF2.
RC TISSUE=Macrophage;
RX PubMed=8707840; DOI=10.1083/jcb.134.3.589;
RA Hu T., Guan T., Gerace L.;
RT "Molecular and functional characterization of the p62 complex, an assembly
RT of nuclear pore complex glycoproteins.";
RL J. Cell Biol. 134:589-601(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the nuclear pore complex, a complex required for
CC the trafficking across the nuclear membrane.
CC {ECO:0000269|PubMed:8707840}.
CC -!- SUBUNIT: Component of the p62 complex, a complex composed of NUP62,
CC NUP54, and the isoform p58 and isoform p45 of NUP58. Interacts with
CC NUTF2. {ECO:0000269|PubMed:8707840}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000269|PubMed:8707840}. Nucleus membrane
CC {ECO:0000269|PubMed:8707840}; Peripheral membrane protein
CC {ECO:0000269|PubMed:8707840}; Cytoplasmic side
CC {ECO:0000269|PubMed:8707840}. Nucleus membrane
CC {ECO:0000269|PubMed:8707840}; Peripheral membrane protein
CC {ECO:0000269|PubMed:8707840}; Nucleoplasmic side
CC {ECO:0000269|PubMed:8707840}. Note=Biased towards cytoplasmic side.
CC Central region of the nuclear pore complex, within the transporter.
CC -!- DOMAIN: Contains FG repeats.
CC -!- PTM: O-glycosylated.
CC -!- SIMILARITY: Belongs to the NUP54 family. {ECO:0000305}.
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DR EMBL; U63840; AAC52790.1; -; mRNA.
DR EMBL; BC078858; AAH78858.1; -; mRNA.
DR RefSeq; NP_059057.1; NM_017361.1.
DR PDB; 3T97; X-ray; 2.80 A; B=346-407.
DR PDB; 3T98; X-ray; 2.50 A; A/C=445-494.
DR PDB; 4J3H; X-ray; 1.50 A; A/B=453-494.
DR PDBsum; 3T97; -.
DR PDBsum; 3T98; -.
DR PDBsum; 4J3H; -.
DR AlphaFoldDB; P70582; -.
DR SMR; P70582; -.
DR BioGRID; 248440; 2.
DR CORUM; P70582; -.
DR STRING; 10116.ENSRNOP00000003070; -.
DR jPOST; P70582; -.
DR PaxDb; P70582; -.
DR PRIDE; P70582; -.
DR Ensembl; ENSRNOT00000003070; ENSRNOP00000003070; ENSRNOG00000002247.
DR GeneID; 53372; -.
DR KEGG; rno:53372; -.
DR UCSC; RGD:619926; rat.
DR CTD; 53371; -.
DR RGD; 619926; Nup54.
DR eggNOG; KOG3091; Eukaryota.
DR GeneTree; ENSGT00390000013620; -.
DR HOGENOM; CLU_033371_0_0_1; -.
DR InParanoid; P70582; -.
DR OMA; VYNKVNE; -.
DR OrthoDB; 1506658at2759; -.
DR PhylomeDB; P70582; -.
DR TreeFam; TF320237; -.
DR Reactome; R-RNO-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-RNO-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-RNO-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-RNO-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-RNO-170822; Regulation of Glucokinase by Glucokinase Regulatory Protein.
DR Reactome; R-RNO-191859; snRNP Assembly.
DR Reactome; R-RNO-3108214; SUMOylation of DNA damage response and repair proteins.
DR Reactome; R-RNO-3232142; SUMOylation of ubiquitinylation proteins.
DR Reactome; R-RNO-3301854; Nuclear Pore Complex (NPC) Disassembly.
DR Reactome; R-RNO-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-RNO-4085377; SUMOylation of SUMOylation proteins.
DR Reactome; R-RNO-4551638; SUMOylation of chromatin organization proteins.
DR Reactome; R-RNO-4570464; SUMOylation of RNA binding proteins.
DR Reactome; R-RNO-4615885; SUMOylation of DNA replication proteins.
DR Reactome; R-RNO-5578749; Transcriptional regulation by small RNAs.
DR PRO; PR:P70582; -.
DR Proteomes; UP000002494; Chromosome 14.
DR Bgee; ENSRNOG00000002247; Expressed in thymus and 19 other tissues.
DR Genevisible; P70582; RN.
DR GO; GO:0005635; C:nuclear envelope; IDA:RGD.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005643; C:nuclear pore; IDA:UniProtKB.
DR GO; GO:0044613; C:nuclear pore central transport channel; IBA:GO_Central.
DR GO; GO:0032991; C:protein-containing complex; IDA:RGD.
DR GO; GO:0042802; F:identical protein binding; IPI:RGD.
DR GO; GO:0044877; F:protein-containing complex binding; IDA:RGD.
DR GO; GO:0017056; F:structural constituent of nuclear pore; IDA:UniProtKB.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IBA:GO_Central.
DR GO; GO:0006999; P:nuclear pore organization; IBA:GO_Central.
DR GO; GO:0006913; P:nucleocytoplasmic transport; IDA:UniProtKB.
DR GO; GO:0036228; P:protein localization to nuclear inner membrane; IBA:GO_Central.
DR GO; GO:0006605; P:protein targeting; IDA:UniProtKB.
DR GO; GO:0042306; P:regulation of protein import into nucleus; IDA:RGD.
DR InterPro; IPR024864; Nup54/Nup57/Nup44.
DR InterPro; IPR025712; Nup54_alpha-helical_dom.
DR InterPro; IPR040985; Nup54_C.
DR PANTHER; PTHR13000; PTHR13000; 1.
DR Pfam; PF13874; Nup54; 1.
DR Pfam; PF18437; Nup54_C; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Glycoprotein; Membrane;
KW mRNA transport; Nuclear pore complex; Nucleus; Protein transport;
KW Reference proteome; Repeat; Translocation; Transport.
FT CHAIN 1..510
FT /note="Nuclear pore complex protein Nup54"
FT /id="PRO_0000204876"
FT REPEAT 5..6
FT /note="1"
FT REPEAT 25..26
FT /note="2"
FT REPEAT 28..29
FT /note="3"
FT REPEAT 61..62
FT /note="4"
FT REPEAT 63..64
FT /note="5"
FT REPEAT 67..68
FT /note="6"
FT REPEAT 87..88
FT /note="7"
FT REPEAT 447..448
FT /note="8"
FT REGION 5..448
FT /note="8 X 2 AA repeats of F-G"
FT HELIX 347..401
FT /evidence="ECO:0007829|PDB:3T97"
FT HELIX 460..488
FT /evidence="ECO:0007829|PDB:4J3H"
SQ SEQUENCE 510 AA; 55745 MW; 10454CFED967B910 CRC64;
MAFNFGAPSG TSGTSTATAA PAGGFGGFGT TTTTAGSAFS FSAPTNTGST GLLGGTQNKG
FGFGTGFGTS TGTGTGLGTG LGTGLGFGGF NTQQQQQQQQ TSLGGLFSQP AQAPAQSNQL
INTASALSAP TLLGDERDAI LAKWNQLQAF WGTGKGYFNN NIPPVEFTQE NPFCRFKAVG
YSCMPNNKDE DGLVVLIFNK KETDIRSQQQ QLVESLHKVL GGNQTLTVNV EGIKTLPDDQ
TEVVIYIVER SPNGTSRRVP ATTLYAHFEQ ANIKTQLQQL GVTLSMTRTE LSPAQIKQLL
QNPPAGVDPI IWEQAKVDNP DSEKLIPVPM VGFKELLRRL KVQDQMTKQH QTRLDIISED
ISELQKNQTT TMAKIAQYKR KLMDLSHRTL QVLIKQEIQR KSGYAIQAEE EQLRVQLDTI
QGELNAPTQF KGRLNELMSQ IRMQNHFGAV KSEEKYYIDA DLLREIKQHL KQQQEGLSHL
ISIIKDDLED IKLVEHGLNE TIHSRGGVFS
