NUP58_ARATH
ID NUP58_ARATH Reviewed; 513 AA.
AC Q8RWH9; O23173;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Nuclear pore complex protein NUP58 {ECO:0000303|PubMed:21189294};
DE AltName: Full=Nucleoporin 58;
DE AltName: Full=Protein TRANSCURVATA1 {ECO:0000303|PubMed:23840761};
GN Name=NUP58 {ECO:0000303|PubMed:21189294};
GN Synonyms=TCU1 {ECO:0000303|PubMed:23840761};
GN OrderedLocusNames=At4g37130 {ECO:0000312|Araport:AT4G37130};
GN ORFNames=C7A10.230 {ECO:0000312|EMBL:CAB16782.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAM13071.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia {ECO:0000312|Proteomes:UP000006548};
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP IDENTIFICATION IN THE NUCLEAR PORE COMPLEX BY MASS SPECTROMETRY,
RP SUBCELLULAR LOCATION, AND NOMENCLATURE.
RX PubMed=21189294; DOI=10.1105/tpc.110.079947;
RA Tamura K., Fukao Y., Iwamoto M., Haraguchi T., Hara-Nishimura I.;
RT "Identification and characterization of nuclear pore complex components in
RT Arabidopsis thaliana.";
RL Plant Cell 22:4084-4097(2010).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND INTERACTION WITH GAI; NUP62; SKP1A AND SKP1B.
RX PubMed=23840761; DOI=10.1371/journal.pone.0067661;
RA Ferrandez-Ayela A., Alonso-Peral M.M., Sanchez-Garcia A.B., Micol-Ponce R.,
RA Perez-Perez J.M., Micol J.L., Ponce M.R.;
RT "Arabidopsis TRANSCURVATA1 encodes NUP58, a component of the nucleopore
RT central channel.";
RL PLoS ONE 8:E67661-E67661(2013).
CC -!- FUNCTION: Involved in nucleocytoplasmic trafficking. May have
CC regulatory roles in the gibberellin pathway, in auxin signaling and in
CC light perception. {ECO:0000269|PubMed:23840761}.
CC -!- SUBUNIT: Part of the nuclear pore complex (NPC). The NPC has an eight-
CC fold symmetrical structure comprising a central transport channel and
CC two rings, the cytoplasmic and nuclear rings, to which eight filaments
CC are attached. The cytoplasmic filaments have loose ends, while the
CC nuclear filaments are joined in a distal ring, forming a nuclear
CC basket. NPCs are highly dynamic in configuration and composition, and
CC can be devided in 3 subcomplexes, the NUP62 subcomplex, the NUP107-160
CC subcomplex and the NUP93 subcomplex, containing approximately 30
CC different nucleoporin proteins. Interacts with GAI, NUP62, SKP1A and
CC SKP1B. {ECO:0000269|PubMed:23840761, ECO:0000305|PubMed:21189294}.
CC -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000269|PubMed:21189294,
CC ECO:0000269|PubMed:23840761}. Nucleus, nuclear pore complex
CC {ECO:0000305|PubMed:21189294}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. Higherst expression in cauline leaves,
CC lowest in roots. {ECO:0000269|PubMed:23840761}.
CC -!- DEVELOPMENTAL STAGE: Detected at all developmental stages.
CC {ECO:0000269|PubMed:23840761}.
CC -!- DOMAIN: Contains FG repeats. FG repeats are interaction sites for
CC karyopherins (importins, exportins) and form probably an affinity
CC gradient, guiding the transport proteins unidirectionally with their
CC cargo through the NPC. FG repeat regions are highly flexible and lack
CC ordered secondary structure. The overall conservation of FG repeats
CC regarding exact sequence, spacing, and repeat unit length is limited.
CC {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Pleiotropic phenotype, including early flowering,
CC increased petiole length and leaf lamina folding toward the abaxial
CC surface in an asymmetrical manner relative to the primary vein.
CC Hypersensitivity to 2,4-dichlorophenoxyacetic acid and paclobutrazol.
CC {ECO:0000269|PubMed:23840761}.
CC -!- SIMILARITY: Belongs to the NUP58 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB16782.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80379.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Z99707; CAB16782.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161590; CAB80379.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86757.1; -; Genomic_DNA.
DR EMBL; AY093072; AAM13071.1; -; mRNA.
DR EMBL; BT008879; AAP68318.1; -; mRNA.
DR PIR; F85438; F85438.
DR RefSeq; NP_195430.2; NM_119876.4.
DR AlphaFoldDB; Q8RWH9; -.
DR SMR; Q8RWH9; -.
DR BioGRID; 15148; 36.
DR STRING; 3702.AT4G37130.1; -.
DR PaxDb; Q8RWH9; -.
DR PRIDE; Q8RWH9; -.
DR ProteomicsDB; 248854; -.
DR EnsemblPlants; AT4G37130.1; AT4G37130.1; AT4G37130.
DR GeneID; 829867; -.
DR Gramene; AT4G37130.1; AT4G37130.1; AT4G37130.
DR KEGG; ath:AT4G37130; -.
DR Araport; AT4G37130; -.
DR TAIR; locus:2115055; AT4G37130.
DR eggNOG; ENOG502QR16; Eukaryota.
DR HOGENOM; CLU_026411_0_0_1; -.
DR InParanoid; Q8RWH9; -.
DR OMA; ANYSTKW; -.
DR OrthoDB; 1573765at2759; -.
DR PhylomeDB; Q8RWH9; -.
DR PRO; PR:Q8RWH9; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8RWH9; baseline and differential.
DR Genevisible; Q8RWH9; AT.
DR GO; GO:0005635; C:nuclear envelope; IDA:TAIR.
DR GO; GO:0005643; C:nuclear pore; IBA:GO_Central.
DR GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR GO; GO:0017056; F:structural constituent of nuclear pore; IBA:GO_Central.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR024882; Nucleoporin_p58/p45.
DR PANTHER; PTHR13437; PTHR13437; 1.
PE 1: Evidence at protein level;
KW mRNA transport; Nuclear pore complex; Nucleus; Protein transport;
KW Reference proteome; Repeat; Translocation; Transport.
FT CHAIN 1..513
FT /note="Nuclear pore complex protein NUP58"
FT /id="PRO_0000431088"
FT REPEAT 446..447
FT /note="1"
FT REPEAT 458..459
FT /note="2"
FT REPEAT 466..467
FT /note="3"
FT REPEAT 473..474
FT /note="4"
FT REPEAT 486..487
FT /note="5"
FT REPEAT 497..498
FT /note="6"
FT REGION 28..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 217..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 356..513
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 446..498
FT /note="6 X 2 AA repeats of F-G"
FT COMPBIAS 373..505
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 513 AA; 56542 MW; AEDDC5826370588F CRC64;
MSFFPPQQQQ TPQPLFQTQQ TSLFQPQQTN SIFSQSQPQQ TNSIFSQSQP QQTNSIFSQP
QQQQQTSLFQ PQQFQQQQQQ LNQQQQQQVQ QQLYLFTNDK APANYSTKWA DLHPDSQKLL
LQIEEKILEH RSESQRLDQC SRLYDSSVSS EGFEFDASRI VQELGGINTA MDRQKAVLHE
LMIVAKDMLR NAEIAVRSFM MLQPRFPHWK QGGGVVSVGS QPSQGQGTNP APASSGQQQA
VTTTVQVSDF YRGIPKKPTA FLLQTVVRFE KYLNECRQWV EELEQLLALD SDKYSRHASL
LESLPKVMSN VHDFFVHVAA KVESIHQYIE SMRTSYLADQ RRRGECHDPF LEADRRETAK
QEAAAKRVHP TLHLPASTTS TQPSTQVAGL IASSATPGGS NPPQTSVPTS NPSSGAGFSF
LNTPASGPSS SLFATPSSTA PTSSLFGPSP TPTQTPLFGS SPASTFGSTQ SLFGQTTPSL
TMPSQFGGAT PGSGASFGSM TKSSRPKSRT TRR
