NUP58_MOUSE
ID NUP58_MOUSE Reviewed; 587 AA.
AC Q8R332; Q3UJF4; Q8BUA7; Q8BVG7; Q8C0W3;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Nucleoporin p58/p45 {ECO:0000305};
DE AltName: Full=58 kDa nucleoporin {ECO:0000250|UniProtKB:Q9BVL2};
DE AltName: Full=Nucleoporin-like protein 1;
GN Name=Nup58 {ECO:0000250|UniProtKB:Q9BVL2}; Synonyms=Nupl1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
RC STRAIN=C57BL/6J; TISSUE=Heart, Olfactory bulb, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the nuclear pore complex, a complex required for
CC the trafficking across the nuclear membrane.
CC {ECO:0000250|UniProtKB:P70581}.
CC -!- SUBUNIT: Component of the p62 complex, a complex at least composed of
CC NUP62, NUP54, and NUP58. Interacts with NUTF2. Interacts with SRP1-
CC alpha and Importin p97 proteins when they are together, but not with
CC SRP1-alpha protein alone (By similarity).
CC {ECO:0000250|UniProtKB:P70581}.
CC -!- INTERACTION:
CC Q8R332-1; P63085: Mapk1; NbExp=3; IntAct=EBI-646962, EBI-397697;
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000250|UniProtKB:P70581}. Nucleus membrane
CC {ECO:0000250|UniProtKB:P70581}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P70581}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:P70581}. Nucleus membrane
CC {ECO:0000250|UniProtKB:P70581}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P70581}; Nucleoplasmic side
CC {ECO:0000250|UniProtKB:P70581}. Note=Biased towards cytoplasmic side.
CC Central region of the nuclear pore complex, within the transporter.
CC {ECO:0000250|UniProtKB:P70581}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1; Synonyms=p58;
CC IsoId=Q8R332-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8R332-2; Sequence=VSP_008573;
CC Name=3;
CC IsoId=Q8R332-3; Sequence=VSP_008574;
CC Name=4;
CC IsoId=Q8R332-4; Sequence=VSP_008575;
CC -!- DOMAIN: Contains FG repeats. FG repeats are interaction sites for
CC karyopherins (importins, exportins) and form probably an affinity
CC gradient, guiding the transport proteins unidirectionally with their
CC cargo through the NPC. FG repeat regions are highly flexible and lack
CC ordered secondary structure. The overall conservation of FG repeats
CC regarding exact sequence, spacing, and repeat unit length is limited.
CC {ECO:0000305}.
CC -!- PTM: O-glycosylated. {ECO:0000250}.
CC -!- MISCELLANEOUS: In rat, the p62 complex contains two different isoforms
CC of NUP58. Isoform p45 has however not been isolated in mouse so far.
CC -!- SIMILARITY: Belongs to the NUP58 family. {ECO:0000305}.
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DR EMBL; AK029688; BAC26564.1; -; mRNA.
DR EMBL; AK078279; BAC37200.1; -; mRNA.
DR EMBL; AK086412; BAC39663.1; -; mRNA.
DR EMBL; AK146477; BAE27201.1; -; mRNA.
DR EMBL; BC026743; AAH26743.1; -; mRNA.
DR CCDS; CCDS27175.1; -. [Q8R332-1]
DR CCDS; CCDS88679.1; -. [Q8R332-3]
DR CCDS; CCDS88680.1; -. [Q8R332-2]
DR RefSeq; NP_733479.1; NM_170591.1. [Q8R332-1]
DR AlphaFoldDB; Q8R332; -.
DR SMR; Q8R332; -.
DR BioGRID; 214973; 1.
DR ComplexPortal; CPX-4474; Nuclear pore complex.
DR IntAct; Q8R332; 2.
DR STRING; 10090.ENSMUSP00000038716; -.
DR iPTMnet; Q8R332; -.
DR PhosphoSitePlus; Q8R332; -.
DR EPD; Q8R332; -.
DR MaxQB; Q8R332; -.
DR PaxDb; Q8R332; -.
DR PRIDE; Q8R332; -.
DR ProteomicsDB; 294251; -. [Q8R332-1]
DR ProteomicsDB; 294252; -. [Q8R332-2]
DR ProteomicsDB; 294253; -. [Q8R332-3]
DR ProteomicsDB; 294254; -. [Q8R332-4]
DR DNASU; 71844; -.
DR Ensembl; ENSMUST00000041905; ENSMUSP00000038716; ENSMUSG00000114797. [Q8R332-1]
DR Ensembl; ENSMUST00000225111; ENSMUSP00000153625; ENSMUSG00000114797. [Q8R332-3]
DR Ensembl; ENSMUST00000225311; ENSMUSP00000153117; ENSMUSG00000114797. [Q8R332-2]
DR Ensembl; ENSMUST00000225805; ENSMUSP00000153642; ENSMUSG00000063895. [Q8R332-4]
DR GeneID; 71844; -.
DR KEGG; mmu:71844; -.
DR UCSC; uc007uex.1; mouse. [Q8R332-2]
DR UCSC; uc007uey.1; mouse. [Q8R332-3]
DR UCSC; uc007uez.1; mouse. [Q8R332-1]
DR CTD; 71844; -.
DR MGI; MGI:1919094; Nupl1.
DR VEuPathDB; HostDB:ENSMUSG00000063895; -.
DR VEuPathDB; HostDB:ENSMUSG00000114797; -.
DR eggNOG; ENOG502QRD8; Eukaryota.
DR GeneTree; ENSGT00730000111111; -.
DR HOGENOM; CLU_034851_1_0_1; -.
DR InParanoid; Q8R332; -.
DR OMA; NNRHIKQ; -.
DR OrthoDB; 885529at2759; -.
DR PhylomeDB; Q8R332; -.
DR TreeFam; TF106502; -.
DR Reactome; R-MMU-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-MMU-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-MMU-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-MMU-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-MMU-170822; Regulation of Glucokinase by Glucokinase Regulatory Protein.
DR Reactome; R-MMU-191859; snRNP Assembly.
DR Reactome; R-MMU-3108214; SUMOylation of DNA damage response and repair proteins.
DR Reactome; R-MMU-3232142; SUMOylation of ubiquitinylation proteins.
DR Reactome; R-MMU-3301854; Nuclear Pore Complex (NPC) Disassembly.
DR Reactome; R-MMU-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-MMU-4085377; SUMOylation of SUMOylation proteins.
DR Reactome; R-MMU-4551638; SUMOylation of chromatin organization proteins.
DR Reactome; R-MMU-4570464; SUMOylation of RNA binding proteins.
DR Reactome; R-MMU-4615885; SUMOylation of DNA replication proteins.
DR Reactome; R-MMU-5578749; Transcriptional regulation by small RNAs.
DR BioGRID-ORCS; 71844; 10 hits in 72 CRISPR screens.
DR ChiTaRS; Nupl1; mouse.
DR PRO; PR:Q8R332; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8R332; protein.
DR Bgee; ENSMUSG00000063895; Expressed in spermatid and 5 other tissues.
DR Genevisible; Q8R332; MM.
DR GO; GO:0005635; C:nuclear envelope; ISO:MGI.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005643; C:nuclear pore; ISO:MGI.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0008139; F:nuclear localization sequence binding; ISO:MGI.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR GO; GO:0017056; F:structural constituent of nuclear pore; ISO:MGI.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0006913; P:nucleocytoplasmic transport; ISO:MGI.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0042306; P:regulation of protein import into nucleus; ISO:MGI.
DR InterPro; IPR024882; Nucleoporin_p58/p45.
DR PANTHER; PTHR13437; PTHR13437; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Glycoprotein; Membrane; mRNA transport;
KW Nuclear pore complex; Nucleus; Phosphoprotein; Protein transport;
KW Reference proteome; Repeat; Translocation; Transport.
FT CHAIN 1..587
FT /note="Nucleoporin p58/p45"
FT /id="PRO_0000204893"
FT REPEAT 7..8
FT /note="1"
FT REPEAT 30..31
FT /note="2"
FT REPEAT 44..45
FT /note="3"
FT REPEAT 63..64
FT /note="4"
FT REPEAT 68..69
FT /note="5"
FT REPEAT 476..477
FT /note="6"
FT REPEAT 480..481
FT /note="7"
FT REPEAT 501..502
FT /note="8"
FT REPEAT 507..508
FT /note="9"
FT REPEAT 517..518
FT /note="10"
FT REPEAT 519..520
FT /note="11"
FT REPEAT 533..534
FT /note="12"
FT REPEAT 556..557
FT /note="13"
FT REPEAT 566..567
FT /note="14"
FT REGION 7..567
FT /note="14 X 2 AA repeats of F-G"
FT REGION 196..236
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 565..587
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 244..264
FT /evidence="ECO:0000255"
FT COILED 302..369
FT /evidence="ECO:0000255"
FT COMPBIAS 196..211
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 212..232
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 319
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9BVL2"
FT VAR_SEQ 533..587
FT /note="FGSSSTSGFNFSNPGITASAGLTFGVSNPASAGFGTGGQLLQLKRPPAGNKR
FT GKR -> QKSHVLTGFRKPSVMETNELGRKIQF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_008573"
FT VAR_SEQ 533..587
FT /note="FGSSSTSGFNFSNPGITASAGLTFGVSNPASAGFGTGGQLLQLKRPPAGNKR
FT GKR -> LCASA (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_008574"
FT VAR_SEQ 533..587
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_008575"
FT CONFLICT 55
FT /note="S -> Y (in Ref. 1; BAC26564)"
FT /evidence="ECO:0000305"
FT CONFLICT 78
FT /note="L -> V (in Ref. 1; BAC26564)"
FT /evidence="ECO:0000305"
FT CONFLICT 117
FT /note="T -> A (in Ref. 1; BAC39663)"
FT /evidence="ECO:0000305"
FT CONFLICT 143
FT /note="L -> F (in Ref. 1; BAC37200)"
FT /evidence="ECO:0000305"
FT CONFLICT 513
FT /note="G -> E (in Ref. 1; BAC26564)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 587 AA; 59445 MW; 697E22078331BF0A CRC64;
MATGFSFGSG TLGSTTVAPG GTGAGSGFSF GTVASSTPSV GLNFGSLGSS VTPASTSASA
GGFGTGLFGS KPATGFTLGG TSAGTAATTS ASTTGFSLGF SKPAASATPF ALPVTSTSAS
GLTLSSALTS TPAASTGFTL NNLGATPATT TTASTGLSLG GALAGLGGSL FQSGNTATSG
LGQNALSLSL GTTAPTSAAS NEGLGGIDFS TSSDKKSDKT GTRPEDSKAL KDENLPPVIC
QDVENLQKFV KEQKQVQEEI SRMSSKAMLK VQEDIKALKQ LLSLAASGLQ RNTLNIDKLK
LETAQELKNA EIALRTQKTP PGLQHENTAP ADYFRILVQQ FEVQLQQYRQ QIEELENHLA
TQASNSHITP QDLSMAMQKI YQTFVALAAQ LQSIHENVKV LKEQYLGYRK MFLGDAVDVF
EARRTEAKKW QNAPRVTTGP TPFSTMPNAA AVAMAATLTQ QQQPATGPQP SLGVSFGTPF
GSGIGTGLQS SGLGSSNLGG FGTSSGFGCG TTGASTFGFG TTDKPSGSLS AGFGSSSTSG
FNFSNPGITA SAGLTFGVSN PASAGFGTGG QLLQLKRPPA GNKRGKR
