NUP58_RAT
ID NUP58_RAT Reviewed; 585 AA.
AC P70581; Q9JHE1; Q9QWK7; Q9Z2W7;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Nucleoporin p58/p45 {ECO:0000305};
DE AltName: Full=58 kDa nucleoporin {ECO:0000312|RGD:631334};
DE AltName: Full=Nucleoporin-like protein 1;
GN Name=Nup58 {ECO:0000312|RGD:631334}; Synonyms=Nupl1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM P58), PROTEIN SEQUENCE OF 227-237;
RP 317-333; 409-422 AND 427-433, ALTERNATIVE SPLICING, FUNCTION, SUBCELLULAR
RP LOCATION, IDENTIFICATION IN A COMPLEX WITH NUP62 AND NUP54, AND INTERACTION
RP WITH NUTF2.
RC TISSUE=Macrophage;
RX PubMed=8707840; DOI=10.1083/jcb.134.3.589;
RA Hu T., Guan T., Gerace L.;
RT "Molecular and functional characterization of the p62 complex, an assembly
RT of nuclear pore complex glycoproteins.";
RL J. Cell Biol. 134:589-601(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS P45 AND P23), NUCLEOTIDE SEQUENCE OF
RP 309-585 (ISOFORM H6), AND TISSUE SPECIFICITY.
RC TISSUE=Macrophage;
RX PubMed=9795236; DOI=10.1016/s0378-1119(98)00467-3;
RA Hu T., Gerace L.;
RT "cDNA cloning and analysis of the expression of nucleoporin p45.";
RL Gene 221:245-253(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM P58).
RC TISSUE=Heart;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the nuclear pore complex, a complex required for
CC the trafficking across the nuclear membrane.
CC {ECO:0000269|PubMed:8707840}.
CC -!- SUBUNIT: Component of the p62 complex, a complex composed of NUP62,
CC NUP54, and isoform p58 and isoform p45 of NUP58. Isoform p58 interacts
CC with NUTF2. Isoform p58 interacts with SRP1-alpha and Importin p97
CC proteins when they are together, but not with SRP1-alpha protein alone.
CC {ECO:0000269|PubMed:8707840}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000269|PubMed:8707840}. Nucleus membrane
CC {ECO:0000269|PubMed:8707840}; Peripheral membrane protein
CC {ECO:0000269|PubMed:8707840}; Cytoplasmic side
CC {ECO:0000269|PubMed:8707840}. Nucleus membrane
CC {ECO:0000269|PubMed:8707840}; Peripheral membrane protein
CC {ECO:0000269|PubMed:8707840}; Nucleoplasmic side
CC {ECO:0000269|PubMed:8707840}. Note=Biased towards cytoplasmic side.
CC Central region of the nuclear pore complex, within the transporter.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=p58;
CC IsoId=P70581-1; Sequence=Displayed;
CC Name=p45;
CC IsoId=P70581-2; Sequence=VSP_008579, VSP_008580, VSP_008581;
CC Name=p23;
CC IsoId=P70581-3; Sequence=VSP_008582, VSP_008576, VSP_008577,
CC VSP_008578;
CC Name=H6;
CC IsoId=P70581-4; Sequence=VSP_008583, VSP_008584;
CC -!- TISSUE SPECIFICITY: Expressed in liver. {ECO:0000269|PubMed:9795236}.
CC -!- DOMAIN: Contains FG repeats. FG repeats are interaction sites for
CC karyopherins (importins, exportins) and form probably an affinity
CC gradient, guiding the transport proteins unidirectionally with their
CC cargo through the NPC. FG repeat regions are highly flexible and lack
CC ordered secondary structure. The overall conservation of FG repeats
CC regarding exact sequence, spacing, and repeat unit length is limited.
CC {ECO:0000305}.
CC -!- PTM: O-glycosylated.
CC -!- SIMILARITY: Belongs to the NUP58 family. {ECO:0000305}.
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DR EMBL; U63839; AAC52789.1; -; mRNA.
DR EMBL; AH007036; AAC82539.1; -; mRNA.
DR EMBL; AF000900; AAC82318.1; -; mRNA.
DR EMBL; BC085690; AAH85690.1; -; mRNA.
DR EMBL; AF000901; AAC82319.1; -; mRNA.
DR RefSeq; NP_620791.1; NM_139091.2. [P70581-1]
DR PDB; 2OSZ; X-ray; 2.85 A; A/B/C/D=327-415.
DR PDB; 3T98; X-ray; 2.50 A; B=327-415.
DR PDBsum; 2OSZ; -.
DR PDBsum; 3T98; -.
DR AlphaFoldDB; P70581; -.
DR SMR; P70581; -.
DR BioGRID; 251446; 1.
DR CORUM; P70581; -.
DR STRING; 10116.ENSRNOP00000017204; -.
DR jPOST; P70581; -.
DR PaxDb; P70581; -.
DR PRIDE; P70581; -.
DR Ensembl; ENSRNOT00000063943; ENSRNOP00000063452; ENSRNOG00000012644. [P70581-2]
DR Ensembl; ENSRNOT00000095956; ENSRNOP00000084647; ENSRNOG00000012644. [P70581-1]
DR GeneID; 245922; -.
DR KEGG; rno:245922; -.
DR UCSC; RGD:631334; rat. [P70581-1]
DR CTD; 9818; -.
DR RGD; 631334; Nup58.
DR eggNOG; ENOG502QRD8; Eukaryota.
DR GeneTree; ENSGT00730000111111; -.
DR HOGENOM; CLU_034851_1_0_1; -.
DR InParanoid; P70581; -.
DR OMA; RWYKATI; -.
DR OrthoDB; 885529at2759; -.
DR PhylomeDB; P70581; -.
DR Reactome; R-RNO-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-RNO-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-RNO-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-RNO-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-RNO-170822; Regulation of Glucokinase by Glucokinase Regulatory Protein.
DR Reactome; R-RNO-191859; snRNP Assembly.
DR Reactome; R-RNO-3108214; SUMOylation of DNA damage response and repair proteins.
DR Reactome; R-RNO-3232142; SUMOylation of ubiquitinylation proteins.
DR Reactome; R-RNO-3301854; Nuclear Pore Complex (NPC) Disassembly.
DR Reactome; R-RNO-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-RNO-4085377; SUMOylation of SUMOylation proteins.
DR Reactome; R-RNO-4551638; SUMOylation of chromatin organization proteins.
DR Reactome; R-RNO-4570464; SUMOylation of RNA binding proteins.
DR Reactome; R-RNO-4615885; SUMOylation of DNA replication proteins.
DR Reactome; R-RNO-5578749; Transcriptional regulation by small RNAs.
DR EvolutionaryTrace; P70581; -.
DR PRO; PR:P70581; -.
DR Proteomes; UP000002494; Chromosome 15.
DR Bgee; ENSRNOG00000012644; Expressed in testis and 19 other tissues.
DR ExpressionAtlas; P70581; baseline and differential.
DR Genevisible; P70581; RN.
DR GO; GO:0005635; C:nuclear envelope; IDA:RGD.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005643; C:nuclear pore; IDA:UniProtKB.
DR GO; GO:0032991; C:protein-containing complex; IDA:RGD.
DR GO; GO:0042802; F:identical protein binding; IPI:RGD.
DR GO; GO:0008139; F:nuclear localization sequence binding; IDA:RGD.
DR GO; GO:0044877; F:protein-containing complex binding; IDA:RGD.
DR GO; GO:0017056; F:structural constituent of nuclear pore; IDA:UniProtKB.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0006913; P:nucleocytoplasmic transport; IDA:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0042306; P:regulation of protein import into nucleus; IDA:RGD.
DR InterPro; IPR024882; Nucleoporin_p58/p45.
DR PANTHER; PTHR13437; PTHR13437; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Coiled coil; Direct protein sequencing;
KW Glycoprotein; Membrane; mRNA transport; Nuclear pore complex; Nucleus;
KW Phosphoprotein; Protein transport; Reference proteome; Repeat;
KW Translocation; Transport.
FT CHAIN 1..585
FT /note="Nucleoporin p58/p45"
FT /id="PRO_0000204894"
FT REPEAT 7..8
FT /note="1"
FT REPEAT 30..31
FT /note="2"
FT REPEAT 42..43
FT /note="3"
FT REPEAT 61..62
FT /note="4"
FT REPEAT 66..67
FT /note="5"
FT REPEAT 474..475
FT /note="6"
FT REPEAT 478..479
FT /note="7"
FT REPEAT 499..500
FT /note="8"
FT REPEAT 505..506
FT /note="9"
FT REPEAT 515..516
FT /note="10"
FT REPEAT 517..518
FT /note="11"
FT REPEAT 531..532
FT /note="12"
FT REPEAT 554..555
FT /note="13"
FT REPEAT 564..565
FT /note="14"
FT REGION 7..565
FT /note="14 X 2 AA repeats of F-G"
FT REGION 194..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 563..585
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 242..262
FT /evidence="ECO:0000255"
FT COILED 300..367
FT /evidence="ECO:0000255"
FT COMPBIAS 194..209
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 210..230
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 317
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9BVL2"
FT VAR_SEQ 1..260
FT /note="Missing (in isoform p23)"
FT /evidence="ECO:0000303|PubMed:9795236"
FT /id="VSP_008582"
FT VAR_SEQ 224..245
FT /note="Missing (in isoform p45)"
FT /evidence="ECO:0000303|PubMed:9795236"
FT /id="VSP_008579"
FT VAR_SEQ 328..337
FT /note="PADYFRVLVQ -> SPDDERLQVH (in isoform p23)"
FT /evidence="ECO:0000303|PubMed:9795236"
FT /id="VSP_008576"
FT VAR_SEQ 466..471
FT /note="PQPSLG -> LNAFKL (in isoform H6)"
FT /evidence="ECO:0000305"
FT /id="VSP_008583"
FT VAR_SEQ 466..468
FT /note="PQP -> DGW (in isoform p23)"
FT /evidence="ECO:0000303|PubMed:9795236"
FT /id="VSP_008577"
FT VAR_SEQ 469..585
FT /note="Missing (in isoform p23)"
FT /evidence="ECO:0000303|PubMed:9795236"
FT /id="VSP_008578"
FT VAR_SEQ 472..585
FT /note="Missing (in isoform H6)"
FT /evidence="ECO:0000305"
FT /id="VSP_008584"
FT VAR_SEQ 531..535
FT /note="FGSSS -> LCASA (in isoform p45)"
FT /evidence="ECO:0000303|PubMed:9795236"
FT /id="VSP_008580"
FT VAR_SEQ 536..585
FT /note="Missing (in isoform p45)"
FT /evidence="ECO:0000303|PubMed:9795236"
FT /id="VSP_008581"
FT HELIX 328..357
FT /evidence="ECO:0007829|PDB:3T98"
FT HELIX 360..363
FT /evidence="ECO:0007829|PDB:3T98"
FT HELIX 368..410
FT /evidence="ECO:0007829|PDB:3T98"
SQ SEQUENCE 585 AA; 59265 MW; 4D5F5C2744A75C3C CRC64;
MATGFSFGSG TLGSTTVAPG GTGTGSGFSF GASSTPSVGL NFGTLGSSAT PASTSTSASG
FGTGLFGSKP GTGFTLGGTS AGTTATTSAS TTGFSLGFSK PAASATPFAL PVTSTTASGL
TLSSALTSAP AASTGFTLNN LGATPATTTA ASTGLSLGGA LAGLGGSLFQ SGNTATSGLG
QNALSLSLGT ATPTSAASSE GLGGIDFSTS SDKKSDKTGT RPEDSKALKD ENLPPVICQD
VENLQKFVKE QKQVQEEISR MSSKAMLKVQ EDIKALKQLL SLAASGLQRN TLNIDKLKLE
TAQELKNAEI ALRTQKTPPG LQHENTAPAD YFRVLVQQFE VQLQQYRQQI EELENHLATQ
ANNSHITPQD LSMAMQKIYQ TFVALAAQLQ SIHENVKVLK EQYLSYRKMF LGDAGDVFEA
RRTEAKKWQN APRVTTGPTP FSTMPNAAAV AMAATLTQQQ QPATGPQPSL GVSFGTPFGS
GIGTGLQSSG LGSSNLGGFG TSSGFGCGTT GASTFGFGTT DKPSGSLSAG FGSSSTSGFN
FSNPGITASA GLTFGVSNPA SAGFGTGGQL LQLKRPPAGN KRGKR
