NUP62_ARATH
ID NUP62_ARATH Reviewed; 739 AA.
AC Q8L7F7; Q9SHD8;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Nuclear pore complex protein NUP62 {ECO:0000303|PubMed:21189294};
DE AltName: Full=Nucleoporin 62 {ECO:0000312|EMBL:AEC10495.1};
DE Short=NP62;
DE AltName: Full=Protein EMBRYO DEFECTIVE 2766;
GN Name=NUP62 {ECO:0000303|PubMed:21189294};
GN Synonyms=EMB2766 {ECO:0000312|EMBL:AEC10495.1};
GN OrderedLocusNames=At2g45000 {ECO:0000312|Araport:AT2G45000};
GN ORFNames=T14P1.20 {ECO:0000312|EMBL:AAL69462.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAM91808.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION IN THE NUCLEAR PORE COMPLEX BY MASS SPECTROMETRY,
RP SUBCELLULAR LOCATION, AND NOMENCLATURE.
RX PubMed=21189294; DOI=10.1105/tpc.110.079947;
RA Tamura K., Fukao Y., Iwamoto M., Haraguchi T., Hara-Nishimura I.;
RT "Identification and characterization of nuclear pore complex components in
RT Arabidopsis thaliana.";
RL Plant Cell 22:4084-4097(2010).
RN [5]
RP INTERACTION WITH KPNB1.
RX PubMed=23582042; DOI=10.1111/tpj.12207;
RA Luo Y., Wang Z., Ji H., Fang H., Wang S., Tian L., Li X.;
RT "An Arabidopsis homolog of importin beta1 is required for ABA response and
RT drought tolerance.";
RL Plant J. 75:377-389(2013).
RN [6]
RP INTERACTION WITH NUP58.
RX PubMed=23840761; DOI=10.1371/journal.pone.0067661;
RA Ferrandez-Ayela A., Alonso-Peral M.M., Sanchez-Garcia A.B., Micol-Ponce R.,
RA Perez-Perez J.M., Micol J.L., Ponce M.R.;
RT "Arabidopsis TRANSCURVATA1 encodes NUP58, a component of the nucleopore
RT central channel.";
RL PLoS ONE 8:E67661-E67661(2013).
CC -!- SUBUNIT: Part of the nuclear pore complex (NPC). The NPC has an eight-
CC fold symmetrical structure comprising a central transport channel and
CC two rings, the cytoplasmic and nuclear rings, to which eight filaments
CC are attached. The cytoplasmic filaments have loose ends, while the
CC nuclear filaments are joined in a distal ring, forming a nuclear
CC basket. NPCs are highly dynamic in configuration and composition, and
CC can be devided in 3 subcomplexes, the NUP62 subcomplex, the NUP107-160
CC subcomplex and the NUP93 subcomplex, containing approximately 30
CC different nucleoporin proteins. Interacts with NUP58 and the importin
CC KPNB1. {ECO:0000269|PubMed:23582042, ECO:0000269|PubMed:23840761,
CC ECO:0000305|PubMed:21189294}.
CC -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000269|PubMed:21189294}.
CC Nucleus, nuclear pore complex {ECO:0000305|PubMed:21189294}.
CC -!- DOMAIN: Contains FG repeats. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the nucleoporin NSP1/NUP62 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL69462.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
CC Sequence=AAL86303.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
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DR EMBL; CP002685; AEC10495.1; -; Genomic_DNA.
DR EMBL; AY133874; AAM91808.1; -; mRNA.
DR EMBL; AY074646; AAL69462.1; ALT_SEQ; mRNA.
DR EMBL; AY080620; AAL86303.1; ALT_SEQ; mRNA.
DR PIR; D84885; D84885.
DR RefSeq; NP_850429.1; NM_180098.3.
DR AlphaFoldDB; Q8L7F7; -.
DR SMR; Q8L7F7; -.
DR BioGRID; 4444; 13.
DR IntAct; Q8L7F7; 2.
DR STRING; 3702.AT2G45000.1; -.
DR iPTMnet; Q8L7F7; -.
DR PaxDb; Q8L7F7; -.
DR PRIDE; Q8L7F7; -.
DR ProteomicsDB; 248890; -.
DR EnsemblPlants; AT2G45000.1; AT2G45000.1; AT2G45000.
DR GeneID; 819108; -.
DR Gramene; AT2G45000.1; AT2G45000.1; AT2G45000.
DR KEGG; ath:AT2G45000; -.
DR Araport; AT2G45000; -.
DR TAIR; locus:2055547; AT2G45000.
DR eggNOG; KOG2196; Eukaryota.
DR HOGENOM; CLU_389544_0_0_1; -.
DR InParanoid; Q8L7F7; -.
DR OMA; EMMSKQV; -.
DR OrthoDB; 1420505at2759; -.
DR PRO; PR:Q8L7F7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8L7F7; baseline and differential.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005635; C:nuclear envelope; IDA:TAIR.
DR GO; GO:0034399; C:nuclear periphery; IDA:TAIR.
DR GO; GO:0044613; C:nuclear pore central transport channel; IBA:GO_Central.
DR GO; GO:0005543; F:phospholipid binding; IBA:GO_Central.
DR GO; GO:0017056; F:structural constituent of nuclear pore; IBA:GO_Central.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0010930; P:negative regulation of auxin mediated signaling pathway; IGI:TAIR.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR GO; GO:0006405; P:RNA export from nucleus; IBA:GO_Central.
DR InterPro; IPR026010; NSP1/NUP62.
DR InterPro; IPR007758; Nucleoporin_NSP1_C.
DR PANTHER; PTHR12084; PTHR12084; 1.
DR Pfam; PF05064; Nsp1_C; 1.
PE 1: Evidence at protein level;
KW Coiled coil; mRNA transport; Nuclear pore complex; Nucleus;
KW Protein transport; Reference proteome; Repeat; Translocation; Transport.
FT CHAIN 1..739
FT /note="Nuclear pore complex protein NUP62"
FT /id="PRO_0000431084"
FT REPEAT 6..7
FT /note="1"
FT REPEAT 17..18
FT /note="2"
FT REPEAT 50..51
FT /note="3"
FT REPEAT 52..53
FT /note="4"
FT REPEAT 68..69
FT /note="5"
FT REPEAT 70..71
FT /note="6"
FT REPEAT 78..79
FT /note="7"
FT REPEAT 80..81
FT /note="8"
FT REPEAT 91..92
FT /note="9"
FT REPEAT 93..94
FT /note="10"
FT REPEAT 108..109
FT /note="11"
FT REPEAT 110..111
FT /note="12"
FT REPEAT 124..125
FT /note="13"
FT REPEAT 141..142
FT /note="14"
FT REPEAT 159..160
FT /note="15"
FT REPEAT 174..175
FT /note="16"
FT REPEAT 186..187
FT /note="17"
FT REPEAT 207..208
FT /note="18"
FT REPEAT 221..222
FT /note="19"
FT REPEAT 248..249
FT /note="20"
FT REPEAT 271..272
FT /note="21"
FT REPEAT 280..281
FT /note="22"
FT REPEAT 308..309
FT /note="23"
FT REPEAT 366..367
FT /note="24"
FT REPEAT 426..427
FT /note="25"
FT REPEAT 449..450
FT /note="26"
FT REGION 6..450
FT /note="26 X 2 AA repeats of F-G"
FT REGION 18..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 79..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 281..329
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 341..366
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 399..418
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 471..533
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 584..674
FT /evidence="ECO:0000255"
FT COMPBIAS 471..526
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 739 AA; 73482 MW; CD4DEADB5C786357 CRC64;
MSGFPFGQSN SVGGFSFGSS SATNSSSASS TTSPLSFSFN QSSNPSSTGF GFGSSVSSTP
ASSTTPSFGF GASSTPSFGF GSSASSSTPS FGFGSSASVT PASTTPSFGF GTAASSSAPA
PSLFGSSTTN ASSAAPGSSP FGFVTSSASS TATPSSSLFG APASSAATPS SSPFGAAPAS
GSTPLFGSSP SLFSAPSSAS ASNSSLFGAS SSAATSTSPL FGAPSSATGA TPSFSVASSA
PGSSSSIFGA TGSSPSFSVA SSASGSSPSI FGATGSSPFF GSSSSAGSTP SLFASSSSGA
TTSSPSPFGV STFNSSSTSN TSNASASPFS ASTGFSFLKS TASSTTSSTT PSAPPQTASS
SSSFSFGTSA NSGFNLSTGS SAAPASSTSG AVFSIATTTT TSSSTPAATS APASSAPAST
MAFPSFGVTS SATNTTPASS AATFSTTGFG LASSTPATGS TNSFTGFAVP KTSTPASSSQ
PQTTSPAFSF SLPSSTSTTA PATSSATTTQ TTLVVPSSSG TSTAVAPVAG SPKLPSEITG
KTVEEIIKEW NTELQERTGR FRKQANAIAE WDKRILQNRD VLLRLEIEVA KVVETQSSLE
RQLELIETHQ QEVDKALQSM EEEAERIYND ERKSLLDDEA ASTRDAMYEQ SELVERELEH
MTEQIRSIIQ SVNANQGGEL EAIDGMSPLD VVVRILNNQL SSLMWIDEKA EEFSSRIQKI
ALQGSGGDRE LMAPKHWMS
