NUP85_BOVIN
ID NUP85_BOVIN Reviewed; 656 AA.
AC Q3ZC98; A7E3X3;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Nuclear pore complex protein Nup85;
DE AltName: Full=85 kDa nucleoporin;
DE AltName: Full=Nucleoporin Nup85;
GN Name=NUP85;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Essential component of the nuclear pore complex (NPC) that
CC seems to be required for NPC assembly and maintenance. As part of the
CC NPC Nup107-160 subcomplex plays a role in RNA export and in tethering
CC NUP96/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to
CC be required for spindle assembly during mitosis. NUP85 is required for
CC membrane clustering of CCL2-activated CCR2. Seems to be involved in
CC CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to
CC the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion
CC cascade. Involved in nephrogenesis. {ECO:0000250|UniProtKB:Q9BW27}.
CC -!- SUBUNIT: Component of the nuclear pore complex (NPC). Component of the
CC NPC Nup107-160 subcomplex, consisting of at least NUP107, NUP98/Nup96,
CC NUP160, NUP133, NUP85, NUP37, NUP43 and SEC13. Interacts with NUP160,
CC NUP133 and SEC13. Interacts with NUP37, NUP107 and NUP43. Interacts
CC with CCR2. {ECO:0000250|UniProtKB:Q9BW27}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000250|UniProtKB:Q9BW27}. Chromosome, centromere, kinetochore
CC {ECO:0000250|UniProtKB:Q9BW27}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q9BW27}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q9BW27}. Nucleus membrane
CC {ECO:0000250|UniProtKB:Q9BW27}. Note=During mitosis, localizes to the
CC kinetochores and spindle poles. Upon CCl2 stimulation translocates from
CC the cytoplasm to the membrane and colocalizes with CCR2 at the front of
CC migrating cells. {ECO:0000250|UniProtKB:Q9BW27}.
CC -!- SIMILARITY: Belongs to the nucleoporin Nup85 family. {ECO:0000305}.
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DR EMBL; BT030744; ABS45060.1; -; mRNA.
DR EMBL; BC102703; AAI02704.1; -; mRNA.
DR RefSeq; NP_001029559.1; NM_001034387.1.
DR AlphaFoldDB; Q3ZC98; -.
DR SMR; Q3ZC98; -.
DR STRING; 9913.ENSBTAP00000034574; -.
DR PaxDb; Q3ZC98; -.
DR PRIDE; Q3ZC98; -.
DR Ensembl; ENSBTAT00000034688; ENSBTAP00000034574; ENSBTAG00000016126.
DR GeneID; 510540; -.
DR KEGG; bta:510540; -.
DR CTD; 79902; -.
DR VEuPathDB; HostDB:ENSBTAG00000016126; -.
DR VGNC; VGNC:32364; NUP85.
DR eggNOG; KOG2271; Eukaryota.
DR GeneTree; ENSGT00390000000204; -.
DR HOGENOM; CLU_027342_0_0_1; -.
DR InParanoid; Q3ZC98; -.
DR OMA; ELMEWLN; -.
DR OrthoDB; 588551at2759; -.
DR TreeFam; TF323240; -.
DR Proteomes; UP000009136; Chromosome 19.
DR Bgee; ENSBTAG00000016126; Expressed in granulosa cell and 108 other tissues.
DR ExpressionAtlas; Q3ZC98; baseline and differential.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031080; C:nuclear pore outer ring; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0017056; F:structural constituent of nuclear pore; IBA:GO_Central.
DR GO; GO:0030032; P:lamellipodium assembly; IEA:Ensembl.
DR GO; GO:0048246; P:macrophage chemotaxis; IEA:Ensembl.
DR GO; GO:0006406; P:mRNA export from nucleus; IBA:GO_Central.
DR GO; GO:0072006; P:nephron development; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR InterPro; IPR011502; Nucleoporin_Nup85.
DR PANTHER; PTHR13373; PTHR13373; 1.
DR Pfam; PF07575; Nucleopor_Nup85; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Centromere; Chromosome; Cytoplasm; Cytoskeleton; Kinetochore;
KW Membrane; mRNA transport; Nuclear pore complex; Nucleus; Protein transport;
KW Reference proteome; Translocation; Transport.
FT CHAIN 1..656
FT /note="Nuclear pore complex protein Nup85"
FT /id="PRO_0000324186"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9BW27"
FT MOD_RES 92
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8R480"
FT CONFLICT 353
FT /note="F -> L (in Ref. 1; ABS45060)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 656 AA; 74710 MW; D5F1A80D44C828B9 CRC64;
MEEFDGEPTV TWIPGVNSQK KQMCFDWGPG EMLVCETSFN KKEKSEMVAG CPFIHIIRKD
IDVYSKILRK LFNESHGIFV GLQRIEEELT GKSRKAQLVR VSKNYRSVIR ACMEEMHQFA
VADKDSAIGR QFSSQVSILS AVELIWNLCE ILFIEVAPAG PLLLYLLDWV RLHVCEVDSL
SADVLGSENP SKHESFWNLV TTLVLQGRLD EARQMLSKEA DSNPTSAGMC RVLGDLMRTM
PILSPGNTQT LTELELRWQH WHEECERHLQ DGTFASNPHL ESLCKVLLGD DAALLEHKEL
LSNWYHFLVT RLLYSQPTVK PMDLHLYAQS SLDLFLGGES SPEPLDNILM AAFEFDIHQV
IKECSIALSN WWFVAHLTDL LDHCKLLQSH NLYFGSNMRE FLLLEYASGL FAHHSLWQLG
VDYCDHCPEL GRVSLELHIE RIPLTTEQKA LKVLRVCEQR QMTEQVRSIC KVLAMKAVRN
NRLGSALSWS IRAKDAAFAT LVSDRFLRDY CERGCFSDLD LIDNLGPAMM LSDRLTFLGK
YREFHRLYGE KCFVDAASLL LSLMTSQIAP RSFWMTLLTD ALPLLEQKQV IFSAEQTYEL
LRCLEDLTSG RPLCGEPDAQ QLQDDDIETT KVEILRLALA RNLARSIIKE GSLEGS
