NUP85_RAT
ID NUP85_RAT Reviewed; 656 AA.
AC Q4QQS8;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Nuclear pore complex protein Nup85;
DE AltName: Full=85 kDa nucleoporin;
DE AltName: Full=Nucleoporin Nup85;
DE AltName: Full=Pericentrin-1;
GN Name=Nup85; Synonyms=Pcnt1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Essential component of the nuclear pore complex (NPC) that
CC seems to be required for NPC assembly and maintenance. As part of the
CC NPC Nup107-160 subcomplex plays a role in RNA export and in tethering
CC NUP96/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to
CC be required for spindle assembly during mitosis. NUP85 is required for
CC membrane clustering of CCL2-activated CCR2. Seems to be involved in
CC CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to
CC the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion
CC cascade. Involved in nephrogenesis. {ECO:0000250|UniProtKB:Q9BW27}.
CC -!- SUBUNIT: Component of the nuclear pore complex (NPC). Component of the
CC NPC Nup107-160 subcomplex, consisting of at least NUP107, NUP98/Nup96,
CC NUP160, NUP133, NUP85, NUP37, NUP43 and SEC13. Interacts with NUP160,
CC NUP133 and SEC13. Interacts with NUP37, NUP107 and NUP43. Interacts
CC with CCR2. {ECO:0000250|UniProtKB:Q9BW27}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000250|UniProtKB:Q9BW27}. Chromosome, centromere, kinetochore
CC {ECO:0000250|UniProtKB:Q9BW27}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q9BW27}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q9BW27}. Nucleus membrane
CC {ECO:0000250|UniProtKB:Q9BW27}. Note=During mitosis, localizes to the
CC kinetochores and spindle poles. Upon CCl2 stimulation translocates from
CC the cytoplasm to the membrane and colocalizes with CCR2 at the front of
CC migrating cells. {ECO:0000250|UniProtKB:Q9BW27}.
CC -!- SIMILARITY: Belongs to the nucleoporin Nup85 family. {ECO:0000305}.
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DR EMBL; BC098031; AAH98031.1; -; mRNA.
DR RefSeq; NP_001020572.1; NM_001025401.1.
DR AlphaFoldDB; Q4QQS8; -.
DR SMR; Q4QQS8; -.
DR CORUM; Q4QQS8; -.
DR STRING; 10116.ENSRNOP00000005035; -.
DR iPTMnet; Q4QQS8; -.
DR PhosphoSitePlus; Q4QQS8; -.
DR jPOST; Q4QQS8; -.
DR PaxDb; Q4QQS8; -.
DR PRIDE; Q4QQS8; -.
DR Ensembl; ENSRNOT00000005035; ENSRNOP00000005035; ENSRNOG00000003673.
DR GeneID; 287830; -.
DR KEGG; rno:287830; -.
DR CTD; 79902; -.
DR RGD; 1311942; Nup85.
DR eggNOG; KOG2271; Eukaryota.
DR GeneTree; ENSGT00390000000204; -.
DR HOGENOM; CLU_027342_0_0_1; -.
DR InParanoid; Q4QQS8; -.
DR OMA; ELMEWLN; -.
DR OrthoDB; 588551at2759; -.
DR PhylomeDB; Q4QQS8; -.
DR TreeFam; TF323240; -.
DR Reactome; R-RNO-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-RNO-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-RNO-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-RNO-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-RNO-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-RNO-170822; Regulation of Glucokinase by Glucokinase Regulatory Protein.
DR Reactome; R-RNO-191859; snRNP Assembly.
DR Reactome; R-RNO-2467813; Separation of Sister Chromatids.
DR Reactome; R-RNO-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-RNO-3108214; SUMOylation of DNA damage response and repair proteins.
DR Reactome; R-RNO-3232142; SUMOylation of ubiquitinylation proteins.
DR Reactome; R-RNO-3301854; Nuclear Pore Complex (NPC) Disassembly.
DR Reactome; R-RNO-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-RNO-4085377; SUMOylation of SUMOylation proteins.
DR Reactome; R-RNO-4551638; SUMOylation of chromatin organization proteins.
DR Reactome; R-RNO-4570464; SUMOylation of RNA binding proteins.
DR Reactome; R-RNO-4615885; SUMOylation of DNA replication proteins.
DR Reactome; R-RNO-5578749; Transcriptional regulation by small RNAs.
DR Reactome; R-RNO-5663220; RHO GTPases Activate Formins.
DR Reactome; R-RNO-68877; Mitotic Prometaphase.
DR Reactome; R-RNO-9615933; Postmitotic nuclear pore complex (NPC) reformation.
DR Reactome; R-RNO-9648025; EML4 and NUDC in mitotic spindle formation.
DR PRO; PR:Q4QQS8; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Bgee; ENSRNOG00000003673; Expressed in thymus and 20 other tissues.
DR Genevisible; Q4QQS8; RN.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005635; C:nuclear envelope; ISO:RGD.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031080; C:nuclear pore outer ring; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0017056; F:structural constituent of nuclear pore; IBA:GO_Central.
DR GO; GO:0006935; P:chemotaxis; ISO:RGD.
DR GO; GO:0030032; P:lamellipodium assembly; ISO:RGD.
DR GO; GO:0048246; P:macrophage chemotaxis; ISO:RGD.
DR GO; GO:0006406; P:mRNA export from nucleus; IBA:GO_Central.
DR GO; GO:0072006; P:nephron development; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR InterPro; IPR011502; Nucleoporin_Nup85.
DR PANTHER; PTHR13373; PTHR13373; 1.
DR Pfam; PF07575; Nucleopor_Nup85; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Centromere; Chromosome; Cytoplasm; Cytoskeleton; Kinetochore;
KW Membrane; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein;
KW Protein transport; Reference proteome; Translocation; Transport.
FT CHAIN 1..656
FT /note="Nuclear pore complex protein Nup85"
FT /id="PRO_0000324189"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9BW27"
FT MOD_RES 92
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8R480"
FT MOD_RES 223
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BW27"
SQ SEQUENCE 656 AA; 74881 MW; B2D84CC2DF58CD68 CRC64;
MEELDCEPAV TWIPGVNSKK KQMCFDWGPG EMLLCETSFN KTDKSEKVPS CPFIYIIRKD
VDVYSQILRK LFNESHGIFV GLQRLEEELS GKSRKAQLVR VSKNYRSVIR ACMEEMHQVA
IAAKDPASGR QFSSQVSILS AMELIWNLCE ILFIEVAPAG PLLLHLLDWV RLHVCEVDSL
SADVLGSDHP SKHESFWNLV TVLVLQGRLD EARQMLSKEA DASPSSAGMC RVLGDLMRTM
PILSPGNTQT LTELELKWQH WREECERHLQ DNTFAANPHL ESLCKIMLGD EATLLEQKEL
MSNWYHFLVT RLLYSNPTVK PTDLHLYAQS SLDMFLGGES SPEPLDNILM AAFEFDIHQV
IKECSIALSN WWFVAHLTDL LDHCRLLQSH NLYFGSNMRE FLLLEYASGL FAHHSLWQLG
VDYFDYCPEL GRVSLELHIE RIPLNTEQKA LKVLRICEQR QMTEQVGSIC KILAMKAVRN
NRLGSALSWS IRAKDAAFAT LVSDRFLRDY CERGCFSDLD LIDNLGPAMM LSDRLTFLGK
YREFHRLYGE KRFGDAASLL LSLMTSQIAP RSFWMTLLTD ALPLLEQKQV IFSAEQTYEL
MRCLEDLASR RPECGEPDAQ RLQDDDIETT KVEMLRLALA RNLARAIIRE GSLEGS
