ID   NUP85_SCHPO             Reviewed;         675 AA.
AC   Q9UUE5;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 120.
DE   RecName: Full=Nucleoporin nup85;
DE   AltName: Full=Nuclear pore protein nup85;
GN   Name=nup85; ORFNames=SPBC17G9.04c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   IDENTIFICATION IN NUP107-120 COMPLEX, AND SUBCELLULAR LOCATION.
RX   PubMed=15226438; DOI=10.1128/mcb.24.14.6379-6392.2004;
RA   Bai S.W., Rouquette J., Umeda M., Faigle W., Loew D., Sazer S., Doye V.;
RT   "The fission yeast Nup107-120 complex functionally interacts with the small
RT   GTPase Ran/Spi1 and is required for mRNA export, nuclear pore distribution,
RT   and proper cell division.";
RL   Mol. Cell. Biol. 24:6379-6392(2004).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=15116432; DOI=10.1002/yea.1115;
RA   Chen X.Q., Du X., Liu J., Balasubramanian M.K., Balasundaram D.;
RT   "Identification of genes encoding putative nucleoporins and transport
RT   factors in the fission yeast Schizosaccharomyces pombe: a deletion
RT   analysis.";
RL   Yeast 21:495-509(2004).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- FUNCTION: Functions as a component of the nuclear pore complex (NPC).
CC       NPC components, collectively referred to as nucleoporins (NUPs), can
CC       play the role of both NPC structural components and of docking or
CC       interaction partners for transiently associated nuclear transport
CC       factors. Active directional transport is assured by both, a Phe-Gly
CC       (FG) repeat affinity gradient for these transport factors across the
CC       NPC and a transport cofactor concentration gradient across the nuclear
CC       envelope. {ECO:0000269|PubMed:15116432}.
CC   -!- SUBUNIT: Component of the nuclear pore complex (NPC). NPC constitutes
CC       the exclusive means of nucleocytoplasmic transport. NPCs allow the
CC       passive diffusion of ions and small molecules and the active, nuclear
CC       transport receptor-mediated bidirectional transport of macromolecules
CC       such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal
CC       subunits across the nuclear envelope. Due to its 8-fold rotational
CC       symmetry, all subunits are present with 8 copies or multiples thereof.
CC       {ECO:0000269|PubMed:15116432}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC       envelope {ECO:0000269|PubMed:15116432, ECO:0000269|PubMed:16823372}.
CC       Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole
CC       body {ECO:0000269|PubMed:16823372}. Nucleus, nuclear pore complex
CC       {ECO:0000269|PubMed:15226438}. Note=Localizes to the nuclear envelope
CC       and spindle pole body. {ECO:0000269|PubMed:16823372}.
CC   -!- SIMILARITY: Belongs to the nucleoporin Nup85 family. {ECO:0000305}.
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DR   EMBL; CU329671; CAB52802.1; -; Genomic_DNA.
DR   PIR; T39727; T39727.
DR   RefSeq; NP_595893.1; NM_001021800.2.
DR   AlphaFoldDB; Q9UUE5; -.
DR   SMR; Q9UUE5; -.
DR   BioGRID; 276378; 12.
DR   IntAct; Q9UUE5; 3.
DR   STRING; 4896.SPBC17G9.04c.1; -.
DR   iPTMnet; Q9UUE5; -.
DR   MaxQB; Q9UUE5; -.
DR   PaxDb; Q9UUE5; -.
DR   PRIDE; Q9UUE5; -.
DR   EnsemblFungi; SPBC17G9.04c.1; SPBC17G9.04c.1:pep; SPBC17G9.04c.
DR   GeneID; 2539829; -.
DR   KEGG; spo:SPBC17G9.04c; -.
DR   PomBase; SPBC17G9.04c; nup85.
DR   VEuPathDB; FungiDB:SPBC17G9.04c; -.
DR   eggNOG; KOG2271; Eukaryota.
DR   HOGENOM; CLU_019986_0_0_1; -.
DR   InParanoid; Q9UUE5; -.
DR   OMA; ELMEWLN; -.
DR   PhylomeDB; Q9UUE5; -.
DR   Reactome; R-SPO-159227; Transport of the SLBP independent Mature mRNA.
DR   Reactome; R-SPO-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR   Reactome; R-SPO-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR   Reactome; R-SPO-3232142; SUMOylation of ubiquitinylation proteins.
DR   Reactome; R-SPO-4085377; SUMOylation of SUMOylation proteins.
DR   Reactome; R-SPO-4551638; SUMOylation of chromatin organization proteins.
DR   Reactome; R-SPO-4570464; SUMOylation of RNA binding proteins.
DR   Reactome; R-SPO-5578749; Transcriptional regulation by small RNAs.
DR   Reactome; R-SPO-9615933; Postmitotic nuclear pore complex (NPC) reformation.
DR   PRO; PR:Q9UUE5; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0000791; C:euchromatin; IDA:PomBase.
DR   GO; GO:0000792; C:heterochromatin; IDA:PomBase.
DR   GO; GO:0005635; C:nuclear envelope; HDA:PomBase.
DR   GO; GO:0034399; C:nuclear periphery; IDA:PomBase.
DR   GO; GO:0005643; C:nuclear pore; IDA:PomBase.
DR   GO; GO:0031080; C:nuclear pore outer ring; IDA:PomBase.
DR   GO; GO:0140602; C:nucleolar ring; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0005816; C:spindle pole body; IEA:UniProtKB-SubCell.
DR   GO; GO:1990188; F:euchromatin binding; IDA:PomBase.
DR   GO; GO:0017056; F:structural constituent of nuclear pore; IBA:GO_Central.
DR   GO; GO:0006406; P:mRNA export from nucleus; IMP:PomBase.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IBA:GO_Central.
DR   GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR   GO; GO:0006407; P:rRNA export from nucleus; ISO:PomBase.
DR   InterPro; IPR011502; Nucleoporin_Nup85.
DR   PANTHER; PTHR13373; PTHR13373; 1.
DR   Pfam; PF07575; Nucleopor_Nup85; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Cytoskeleton; mRNA transport; Nuclear pore complex; Nucleus;
KW   Protein transport; Reference proteome; Translocation; Transport.
FT   CHAIN           1..675
FT                   /note="Nucleoporin nup85"
FT                   /id="PRO_0000290672"
SQ   SEQUENCE   675 AA;  78022 MW;  799D29CB87A88FE8 CRC64;
     MESSDEVDLP VYHLQNAPIE NGKLNDWRSK GRTVAFKLHP FLRKGLAYIN NKEFDENTLK
     SDKFEEVESL VYEFSTPSTL IEPNYLLTAW HELWEELQDT YMTPILDSEA NLLLLTQFYG
     KISSLFRSKI IQVLEELQSR INEGEKDCQP VLDSLWEVES AWRCAEAIYF PPSSPYTLST
     GILDWVNAYD PQPIADDGLE IMAYRIPYQH PEFWPYVNKT AIRGLFEQTI SCLEMSGLTK
     EWPVLKETVD ELIDILRYSP CTHQKRIRSV SDFERRWKLW RSRLANLRHV VKKHRDIDSE
     VLDDFVVLLD ILNGNKEVIM LSCAHWQEYF SALAFLYGPL DCKNPEDISL LYQLATGEDS
     KFYVNGTIEY EQICVNLCSN EPLNAIKHAY LLDLGLAVHL ADLLSKSGHL RDYITEEYPI
     TLREHLILEY GQCVLESRNL WQTSFAYWKC VADSGYQRIK ACIPYVPLSD VDAKETALQL
     CKQLKLRDEA QLVLTHWADE LIARNHYGEA LIALDNAANY SALNRVTWEL FDICIAEKKS
     FSPDKDELLY ELFSSPKACT PTLASIISPA ATIHQYFFYL QHKKELNASE LLVGLLTMVD
     FPSSRFPKLL ELLHEFLNNP LQSNSTDFKL SLVNVYDCIA VLQDQQSTVK DQQLLLSIHE
     RLSSAISWYF LHLKK