NUP88_MOUSE
ID NUP88_MOUSE Reviewed; 753 AA.
AC Q8CEC0; Q80Z13; Q8K090;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Nuclear pore complex protein Nup88;
DE AltName: Full=88 kDa nucleoporin;
DE AltName: Full=Nucleoporin Nup88;
GN Name=Nup88;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Spleen;
RA Takahashi N., de Hooge A.S., van de Loo F.A.J.;
RT "Cloning and characterization of mouse nucleoporin 88kDa protein (Nup88).";
RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Skin;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-710, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35 AND THR-526, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of nuclear pore complex.
CC {ECO:0000250|UniProtKB:Q99567}.
CC -!- SUBUNIT: Interacts with NUP214/CAN. Interacts with NUP62 and NUP98.
CC {ECO:0000250|UniProtKB:Q99567}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000250|UniProtKB:Q99567}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8CEC0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CEC0-2; Sequence=VSP_011196;
CC Name=3;
CC IsoId=Q8CEC0-3; Sequence=VSP_011195, VSP_011196;
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DR EMBL; AJ532593; CAD58713.1; -; mRNA.
DR EMBL; AK028563; BAC26010.1; -; mRNA.
DR EMBL; BC032929; AAH32929.1; -; mRNA.
DR CCDS; CCDS36206.1; -. [Q8CEC0-1]
DR CCDS; CCDS36207.1; -. [Q8CEC0-3]
DR CCDS; CCDS70233.1; -. [Q8CEC0-2]
DR RefSeq; NP_001076800.1; NM_001083331.2. [Q8CEC0-1]
DR RefSeq; NP_001263335.1; NM_001276406.1. [Q8CEC0-2]
DR RefSeq; NP_765982.2; NM_172394.3. [Q8CEC0-3]
DR AlphaFoldDB; Q8CEC0; -.
DR SMR; Q8CEC0; -.
DR BioGRID; 202355; 8.
DR ComplexPortal; CPX-4474; Nuclear pore complex.
DR IntAct; Q8CEC0; 6.
DR STRING; 10090.ENSMUSP00000048101; -.
DR iPTMnet; Q8CEC0; -.
DR PhosphoSitePlus; Q8CEC0; -.
DR EPD; Q8CEC0; -.
DR jPOST; Q8CEC0; -.
DR MaxQB; Q8CEC0; -.
DR PaxDb; Q8CEC0; -.
DR PRIDE; Q8CEC0; -.
DR ProteomicsDB; 287859; -. [Q8CEC0-1]
DR ProteomicsDB; 287860; -. [Q8CEC0-2]
DR ProteomicsDB; 287861; -. [Q8CEC0-3]
DR Antibodypedia; 3693; 194 antibodies from 29 providers.
DR DNASU; 19069; -.
DR Ensembl; ENSMUST00000035283; ENSMUSP00000048101; ENSMUSG00000040667. [Q8CEC0-1]
DR Ensembl; ENSMUST00000108530; ENSMUSP00000104170; ENSMUSG00000040667. [Q8CEC0-3]
DR Ensembl; ENSMUST00000108531; ENSMUSP00000104171; ENSMUSG00000040667. [Q8CEC0-2]
DR GeneID; 19069; -.
DR KEGG; mmu:19069; -.
DR UCSC; uc007jww.2; mouse. [Q8CEC0-1]
DR UCSC; uc007jwy.2; mouse. [Q8CEC0-2]
DR CTD; 4927; -.
DR MGI; MGI:104900; Nup88.
DR VEuPathDB; HostDB:ENSMUSG00000040667; -.
DR eggNOG; KOG4460; Eukaryota.
DR GeneTree; ENSGT00390000015063; -.
DR HOGENOM; CLU_017144_0_0_1; -.
DR InParanoid; Q8CEC0; -.
DR OMA; HINQIQL; -.
DR PhylomeDB; Q8CEC0; -.
DR TreeFam; TF105307; -.
DR Reactome; R-MMU-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-MMU-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-MMU-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-MMU-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-MMU-170822; Regulation of Glucokinase by Glucokinase Regulatory Protein.
DR Reactome; R-MMU-191859; snRNP Assembly.
DR Reactome; R-MMU-3108214; SUMOylation of DNA damage response and repair proteins.
DR Reactome; R-MMU-3232142; SUMOylation of ubiquitinylation proteins.
DR Reactome; R-MMU-3301854; Nuclear Pore Complex (NPC) Disassembly.
DR Reactome; R-MMU-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-MMU-4085377; SUMOylation of SUMOylation proteins.
DR Reactome; R-MMU-4551638; SUMOylation of chromatin organization proteins.
DR Reactome; R-MMU-4570464; SUMOylation of RNA binding proteins.
DR Reactome; R-MMU-4615885; SUMOylation of DNA replication proteins.
DR Reactome; R-MMU-5578749; Transcriptional regulation by small RNAs.
DR BioGRID-ORCS; 19069; 21 hits in 73 CRISPR screens.
DR ChiTaRS; Nup88; mouse.
DR PRO; PR:Q8CEC0; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8CEC0; protein.
DR Bgee; ENSMUSG00000040667; Expressed in embryonic post-anal tail and 269 other tissues.
DR ExpressionAtlas; Q8CEC0; baseline and differential.
DR Genevisible; Q8CEC0; MM.
DR GO; GO:0005635; C:nuclear envelope; ISO:MGI.
DR GO; GO:0005643; C:nuclear pore; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0017056; F:structural constituent of nuclear pore; IEA:InterPro.
DR GO; GO:0000278; P:mitotic cell cycle; ISO:MGI.
DR GO; GO:0006406; P:mRNA export from nucleus; IBA:GO_Central.
DR GO; GO:0006913; P:nucleocytoplasmic transport; IC:ComplexPortal.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR GO; GO:0000055; P:ribosomal large subunit export from nucleus; IBA:GO_Central.
DR GO; GO:0000056; P:ribosomal small subunit export from nucleus; IBA:GO_Central.
DR InterPro; IPR019321; Nucleoporin_Nup88.
DR InterPro; IPR037700; NUP88/NUP82.
DR PANTHER; PTHR13257; PTHR13257; 1.
DR Pfam; PF10168; Nup88; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Coiled coil; mRNA transport;
KW Nuclear pore complex; Nucleus; Phosphoprotein; Protein transport;
KW Reference proteome; Translocation; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q99567"
FT CHAIN 2..753
FT /note="Nuclear pore complex protein Nup88"
FT /id="PRO_0000204888"
FT COILED 623..663
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q99567"
FT MOD_RES 35
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 380
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q99567"
FT MOD_RES 438
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q99567"
FT MOD_RES 518
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q99567"
FT MOD_RES 526
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 541
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q99567"
FT MOD_RES 710
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355"
FT VAR_SEQ 550..554
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_011195"
FT VAR_SEQ 618..623
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.1"
FT /id="VSP_011196"
FT CONFLICT 34
FT /note="Q -> H (in Ref. 1; CAD58713)"
FT /evidence="ECO:0000305"
FT CONFLICT 40
FT /note="E -> D (in Ref. 1; CAD58713)"
FT /evidence="ECO:0000305"
FT CONFLICT 86
FT /note="R -> P (in Ref. 1; CAD58713)"
FT /evidence="ECO:0000305"
FT CONFLICT 397
FT /note="H -> D (in Ref. 1; CAD58713)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 753 AA; 84938 MW; F537DCC5D50D140C CRC64;
MAAAVGPLGD GELWQSWLPN HVVFLRLREG VRNQSPAEAE KPAASTSPSC PSLPPHLPTR
NLVFGLGGEL FLWDAEGSAF LVVRLRGPSG GGVEPPLSQY QRLLCINPPL FEIHQVLLSP
TQHHVALIGS KGLMALELPQ RWGKDSEFEG GKATVNCSTI PIAERFFTSS TSLTLKHAAW
YPSEMLDPHI VLLTSDNVIR IYSLREPQTP TKVIVLSEAE EESLILNKGR AYTASLGETA
VAFDFGPLVT VSKNIFEQKD RDVVAYPLYI LYENGETFLT YVSLLHSPGN IGKLLGPLPM
HPAAEDNYGY DACAILCLPC VPNILVIATE SGMLYHCVVL EGEEEDDQTL EKSWDPRADF
IPSLYVFECV ELELALKLAS GEDDPFASDF SCPIKLHRDP KCPSRYHCSH EAGVHSVGLT
WIHKLHKFLG SDEEDKDSLQ ELTAEQKCFV EHILCTKPLP CRQPAPIRGF WIVPDILGPT
MICITSTYEC LIRPLLSTVH PASPPLLCTQ EDAEVAESPL RILAETPDSF EKHIKRILQR
SAANPAFLKN CSARSSEKDL APPPEECLQL ISRATQVFRE QYILKQDLAK EEIQRRVKLL
CDQKRKQLED LNYCREERVS HLFRKSLREM AERLADKYEE AKEKQEDIMN RMKKVLHSFH
AQLPVLSDSE RDMKKELQLI PDQLRHLGNA IKQVTMKKDY QQRKMEKVLS PQKPTITLSA
YQRKCIQSIL KEEGEHIREM VKQINDIRNH VTF
