ID   NUPA_LACLM              Reviewed;         506 AA.
AC   A2RKA7;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Nucleoside import ATP-binding protein NupA {ECO:0000305};
DE            EC=7.6.2.- {ECO:0000305|PubMed:20595258};
GN   Name=nupA {ECO:0000303|PubMed:20595258};
GN   OrderedLocusNames=llmg_1123 {ECO:0000312|EMBL:CAL97717.1};
OS   Lactococcus lactis subsp. cremoris (strain MG1363).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus; Lactococcus cremoris subsp. cremoris.
OX   NCBI_TaxID=416870;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MG1363;
RX   PubMed=17307855; DOI=10.1128/jb.01768-06;
RA   Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C.,
RA   Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P.,
RA   van Sinderen D., Kok J.;
RT   "The complete genome sequence of the lactic acid bacterial paradigm
RT   Lactococcus lactis subsp. cremoris MG1363.";
RL   J. Bacteriol. 189:3256-3270(2007).
RN   [2]
RP   FUNCTION, SUBUNIT, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MG1363;
RX   PubMed=20595258; DOI=10.1099/mic.0.039818-0;
RA   Martinussen J., Soerensen C., Jendresen C.B., Kilstrup M.;
RT   "Two nucleoside transporters in Lactococcus lactis with different substrate
RT   specificities.";
RL   Microbiology 156:3148-3157(2010).
CC   -!- FUNCTION: Part of an ABC transporter complex involved in the uptake of
CC       all common nucleosides (PubMed:20595258). Responsible for energy
CC       coupling to the transport system (Probable).
CC       {ECO:0000269|PubMed:20595258, ECO:0000305}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NupA),
CC       two transmembrane proteins (NupB and NupC) and a solute-binding protein
CC       (BmpA). {ECO:0000305|PubMed:20595258}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Peripheral membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Constitutively expressed. {ECO:0000269|PubMed:20595258}.
CC   -!- DISRUPTION PHENOTYPE: Mutant is unable to grow on inosine as a sole
CC       purine source. Mutant shows slower uptake of uridine. The double mutant
CC       uriP-nupA is impaired in uridine transport.
CC       {ECO:0000269|PubMed:20595258}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR   EMBL; AM406671; CAL97717.1; -; Genomic_DNA.
DR   RefSeq; WP_011835028.1; NZ_WJVF01000012.1.
DR   AlphaFoldDB; A2RKA7; -.
DR   SMR; A2RKA7; -.
DR   STRING; 416870.llmg_1123; -.
DR   TCDB; 3.A.1.2.17; the atp-binding cassette (abc) superfamily.
DR   EnsemblBacteria; CAL97717; CAL97717; llmg_1123.
DR   KEGG; llm:llmg_1123; -.
DR   eggNOG; COG3845; Bacteria.
DR   HOGENOM; CLU_000604_92_0_9; -.
DR   OMA; VGREMSH; -.
DR   PhylomeDB; A2RKA7; -.
DR   BioCyc; LLAC416870:LLMG_RS05705-MON; -.
DR   Proteomes; UP000000364; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProt.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 2.
DR   SMART; SM00382; AAA; 2.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding; Repeat;
KW   Translocase; Transport.
FT   CHAIN           1..506
FT                   /note="Nucleoside import ATP-binding protein NupA"
FT                   /id="PRO_0000449273"
FT   DOMAIN          7..242
FT                   /note="ABC transporter 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   DOMAIN          259..503
FT                   /note="ABC transporter 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         39..46
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ   SEQUENCE   506 AA;  55544 MW;  42E7A35C10D018F4 CRC64;
     MANETVIQMI DVTKRFGDFV ANDKVNLELK KGEIHALLGE NGAGKSTLMN ILSGLLEPSE
     GEVHVKGKLE NIDSPSKAAN LGIGMVHQHF MLVDAFTVTE NIILGNEVTK GINLDLKTAK
     KKILELSERY GLSVEPDALI RDISVGQQQR VEILKTLYRG ADILIFDEPT AVLTPAEITE
     LMQIMKNLIK EGKSIILITH KLDEIRAVAD RITVIRRGKS IDTVELGDKT NQELAELMVG
     RSVSFITEKA AAQPKDVVLE IKDLNIKESR GSLKVKGLSL DVRAGEIVGV AGIDGNGQTE
     LVKAITGLTK VDSGSIKLHN KDITNQRPRK ITEQSVGHVP EDRHRDGLVL EMTVAENIAL
     QTYYKPPMSK YGFLDYNKIN SHARELMEEF DVRGAGEWVS ASSLSGGNQQ KAIIAREIDR
     NPDLLIVSQP TRGLDVGAIE YIHKRLIQAR DEGKAVLVIS FELDEILNVS DRIAVIHDGQ
     IQGIVSPETT TKQELGILMV GGNINE