NUPG_BACSU
ID NUPG_BACSU Reviewed; 397 AA.
AC P42312;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Purine nucleoside transport protein NupG;
GN Name=nupG; Synonyms=yxjA; OrderedLocusNames=BSU39020; ORFNames=N15HR;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / BGSC1A1;
RX PubMed=8969509; DOI=10.1099/13500872-142-11-3113;
RA Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y.,
RA Fujita Y.;
RT "Sequencing of a 65 kb region of the Bacillus subtilis genome containing
RT the lic and cel loci, and creation of a 177 kb contig covering the gnt-
RT sacXY region.";
RL Microbiology 142:3113-3123(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 279.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP FUNCTION IN PURINE NUCLEOSIDE UPTAKE, AND INDUCTION.
RC STRAIN=168;
RX PubMed=12923093; DOI=10.1128/jb.185.17.5200-5209.2003;
RA Johansen L.E., Nygaard P., Lassen C., Agersoe Y., Saxild H.H.;
RT "Definition of a second Bacillus subtilis pur regulon comprising the pur
RT and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).";
RL J. Bacteriol. 185:5200-5209(2003).
CC -!- FUNCTION: Involved in the uptake of the purine ribonucleosides inosine
CC and guanosine. {ECO:0000269|PubMed:12923093}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Expression is regulated by the XptR regulon. Negatively
CC regulated by hypoxanthine and guanine. {ECO:0000269|PubMed:12923093}.
CC -!- SIMILARITY: Belongs to the concentrative nucleoside transporter (CNT)
CC (TC 2.A.41) family. {ECO:0000305}.
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DR EMBL; D83026; BAA11702.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15928.2; -; Genomic_DNA.
DR PIR; G70078; G70078.
DR RefSeq; NP_391781.2; NC_000964.3.
DR RefSeq; WP_003227216.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P42312; -.
DR SMR; P42312; -.
DR IntAct; P42312; 3.
DR STRING; 224308.BSU39020; -.
DR TCDB; 2.A.41.1.3; the concentrative nucleoside transporter (cnt) family.
DR PaxDb; P42312; -.
DR PRIDE; P42312; -.
DR EnsemblBacteria; CAB15928; CAB15928; BSU_39020.
DR GeneID; 937472; -.
DR KEGG; bsu:BSU39020; -.
DR PATRIC; fig|224308.179.peg.4224; -.
DR eggNOG; COG1972; Bacteria.
DR OMA; FITGQPK; -.
DR PhylomeDB; P42312; -.
DR BioCyc; BSUB:BSU39020-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005337; F:nucleoside transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IBA:GO_Central.
DR GO; GO:1901642; P:nucleoside transmembrane transport; IBA:GO_Central.
DR InterPro; IPR008276; C_nuclsd_transpt.
DR InterPro; IPR018270; C_nuclsd_transpt_met_bac.
DR InterPro; IPR011657; CNT_C_dom.
DR InterPro; IPR002668; CNT_N_dom.
DR PANTHER; PTHR10590; PTHR10590; 1.
DR Pfam; PF07662; Nucleos_tra2_C; 1.
DR Pfam; PF01773; Nucleos_tra2_N; 1.
DR TIGRFAMs; TIGR00804; nupC; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..397
FT /note="Purine nucleoside transport protein NupG"
FT /id="PRO_0000070456"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 279
FT /note="I -> N (in Ref. 1; BAA11702)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 397 AA; 43719 MW; 16D230C4BA5DD2C8 CRC64;
MYFLLNLVGL IVIMAVVFLC SPQKKKIKWR PIITLIVLEL LITWFMLGTK VGSWAIGKIG
DFFTWLIACA SDGIAFAFPS VMANETVDFF FSALLPIIFI VTFFDILTYF GILPWLIDKI
GWVISKASRL PKLESFFSIQ MMFLGNTEAL AVIRQQLTVL SNNRLLTFGL MSMSSISGSI
IGSYLSMVPA TYVFTAIPLN CLNALIIANL LNPVHVPEDE DIIYTPPKEE KKDFFSTISN
SMLVGMNMVI VILAMVIGYV ALTSAVNGIL GVFVHGLTIQ TIFAYLFSPF AFLLGLPVHD
AMYVAQLMGM KLATNEFVAM LDLKNNLKSL PPHTVAVATT FLTSFANFST VGMIYGTYNS
ILDGEKSTVI GRNVWKLLVS GIAVSLLSAA IVGLFVW
