ID   NUPO_BACSU              Reviewed;         510 AA.
AC   O05253; Q795M1;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Guanosine import ATP-binding protein NupO {ECO:0000305};
DE            EC=7.6.2.- {ECO:0000305|PubMed:21926227};
GN   Name=nupO {ECO:0000303|PubMed:21926227}; Synonyms=yufO;
GN   OrderedLocusNames=BSU31550;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9274030; DOI=10.1099/00221287-143-8-2769;
RA   Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H.,
RA   Duesterhoeft A., Pohl T.M., Weitzenegger T.;
RT   "Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence
RT   of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).";
RL   Microbiology 143:2769-2774(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION, SUBUNIT, AND INDUCTION.
RC   STRAIN=168 / SMY;
RX   PubMed=21926227; DOI=10.1128/jb.05899-11;
RA   Belitsky B.R., Sonenshein A.L.;
RT   "CodY-mediated regulation of guanosine uptake in Bacillus subtilis.";
RL   J. Bacteriol. 193:6276-6287(2011).
CC   -!- FUNCTION: Part of an ABC transporter complex involved in the uptake of
CC       guanosine (PubMed:21926227). Responsible for energy coupling to the
CC       transport system (Probable). May be a nucleoside transporter of broad
CC       specificity but with various affinities for different substrates
CC       (PubMed:21926227). {ECO:0000269|PubMed:21926227, ECO:0000305}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NupO),
CC       two transmembrane proteins (NupP and NupQ) and a solute-binding protein
CC       (NupN). {ECO:0000305|PubMed:21926227}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Peripheral membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Transcriptionally regulated by CodY.
CC       {ECO:0000269|PubMed:21926227}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z93937; CAB07937.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15144.1; -; Genomic_DNA.
DR   PIR; D70009; D70009.
DR   RefSeq; NP_391033.1; NC_000964.3.
DR   RefSeq; WP_003228830.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O05253; -.
DR   SMR; O05253; -.
DR   IntAct; O05253; 1.
DR   STRING; 224308.BSU31550; -.
DR   PaxDb; O05253; -.
DR   PRIDE; O05253; -.
DR   DNASU; 937172; -.
DR   EnsemblBacteria; CAB15144; CAB15144; BSU_31550.
DR   GeneID; 937172; -.
DR   KEGG; bsu:BSU31550; -.
DR   PATRIC; fig|224308.179.peg.3420; -.
DR   eggNOG; COG3845; Bacteria.
DR   InParanoid; O05253; -.
DR   OMA; VGREMSH; -.
DR   PhylomeDB; O05253; -.
DR   BioCyc; BSUB:BSU31550-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 2.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW   Reference proteome; Repeat; Translocase; Transport.
FT   CHAIN           1..510
FT                   /note="Guanosine import ATP-binding protein NupO"
FT                   /id="PRO_0000360558"
FT   DOMAIN          5..240
FT                   /note="ABC transporter 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   DOMAIN          257..501
FT                   /note="ABC transporter 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         37..44
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ   SEQUENCE   510 AA;  56300 MW;  F75655DDADEE0099 CRC64;
     MEYVIEMLNI RKAFPGIVAN DNINLQVKKG EIHALLGENG AGKSTLMNVL FGLYQPERGE
     IRVRGEKVHI NSPNKANDLG IGMVHQHFML VDTFTVAENI ILGKEPKKFG RIDRKRAGQE
     VQDISDRYGL QIHPEAKAAD ISVGMQQRAE ILKTLYRGAD ILIFDEPTAV LTPHEIKELM
     QIMKNLVKEG KSIILITHKL KEIMEICDRV TVIRKGKGIK TLDVRDTNQD ELASLMVGRE
     VSFKTEKRAA QPGAEVLAID GITVKDTRGI ETVRDLSLSV KAGEIVGIAG VDGNGQSELI
     EAVTGLRKTD SGTITLNGKQ IQNLTPRKIT ESGIGHIPQD RHKHGLVLDF PIGENILLQS
     YYKKPYSALG VLHKGEMYKK ARSLITEYDV RTPDEYTHAR ALSGGNQQKA IIGREIDRNP
     DLLIAAQPTR GLDVGAIEFV HKKLIEQRDA GKAVLLLSFE LEEIMNLSDR IAVIFEGRII
     ASVNPQETTE QELGLLMAGS TQKEAGKANG