NUPX_ECOLI
ID NUPX_ECOLI Reviewed; 416 AA.
AC P33021; Q2MAR8;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Putative nucleoside permease NupX {ECO:0000305};
GN Name=nupX; Synonyms=yeiJ; OrderedLocusNames=b2161, JW2148;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP SUBCELLULAR LOCATION, AND TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the concentrative nucleoside transporter (CNT)
CC (TC 2.A.41) family. {ECO:0000305}.
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DR EMBL; U00007; AAA60513.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75222.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76638.1; -; Genomic_DNA.
DR PIR; H64984; H64984.
DR RefSeq; NP_416666.1; NC_000913.3.
DR RefSeq; WP_000382966.1; NZ_LN832404.1.
DR AlphaFoldDB; P33021; -.
DR SMR; P33021; -.
DR BioGRID; 4260459; 7.
DR DIP; DIP-11922N; -.
DR IntAct; P33021; 1.
DR STRING; 511145.b2161; -.
DR TCDB; 2.A.41.2.10; the concentrative nucleoside transporter (cnt) family.
DR PaxDb; P33021; -.
DR PRIDE; P33021; -.
DR EnsemblBacteria; AAC75222; AAC75222; b2161.
DR EnsemblBacteria; BAE76638; BAE76638; BAE76638.
DR GeneID; 946655; -.
DR KEGG; ecj:JW2148; -.
DR KEGG; eco:b2161; -.
DR PATRIC; fig|1411691.4.peg.78; -.
DR EchoBASE; EB1964; -.
DR eggNOG; COG1972; Bacteria.
DR HOGENOM; CLU_016813_4_2_6; -.
DR InParanoid; P33021; -.
DR OMA; INEFFAY; -.
DR PhylomeDB; P33021; -.
DR BioCyc; EcoCyc:YEIJ-MON; -.
DR BioCyc; MetaCyc:YEIJ-MON; -.
DR PRO; PR:P33021; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0005337; F:nucleoside transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IBA:GO_Central.
DR GO; GO:1901642; P:nucleoside transmembrane transport; IBA:GO_Central.
DR InterPro; IPR008276; C_nuclsd_transpt.
DR InterPro; IPR018270; C_nuclsd_transpt_met_bac.
DR InterPro; IPR011657; CNT_C_dom.
DR InterPro; IPR002668; CNT_N_dom.
DR PANTHER; PTHR10590; PTHR10590; 1.
DR Pfam; PF07662; Nucleos_tra2_C; 1.
DR Pfam; PF01773; Nucleos_tra2_N; 1.
DR TIGRFAMs; TIGR00804; nupC; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..416
FT /note="Putative nucleoside permease NupX"
FT /id="PRO_0000070457"
FT TOPO_DOM 1..2
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 24..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..95
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 96..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..172
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 194..196
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 218..246
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 268..352
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 374..394
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 416
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:15919996"
SQ SEQUENCE 416 AA; 43410 MW; FB8E9C284A028B4D CRC64;
MDVMRSVLGM VVLLTIAFLL SVNKKKISLR TVGAALVLQV VIGGIMLWLP PGRWVAEKVA
FGVHKVMAYS DAGSAFIFGS LVGPKMDTLF DGAGFIFGFR VLPAIIFVTA LVSILYYIGV
MGILIRILGG IFQKALNISK IESFVAVTTI FLGQNEIPAI VKPFIDRLNR NELFTAICSG
MASIAGSTMI GYAALGVPVE YLLAASLMAI PGGILFARLL SPATESSQVS FNNLSFTETP
PKSIIEAAAT GAMTGLKIAA GVATVVMAFV AIIALINGII GGVGGWFGFE HASLESILGY
LLAPLAWVMG VDWSDANLAG SLIGQKLAIN EFVAYLNFSP YLQTAGTLDA KTVAIISFAL
CGFANFGSIG VVVGAFSAVA PHRAPEIAQL GLRALAAATL SNLMSATIAG FFIGLA
