NUR1_YEAST
ID NUR1_YEAST Reviewed; 484 AA.
AC Q12066; D6VRQ9;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Nuclear rim protein 1;
GN Name=NUR1; OrderedLocusNames=YDL089W; ORFNames=D2416;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8923743;
RX DOI=10.1002/(sici)1097-0061(199610)12:13%3c1377::aid-yea35%3e3.0.co;2-r;
RA Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G.,
RA Jimenez A., Remacha M.A.;
RT "The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome
RT IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open
RT reading frames.";
RL Yeast 12:1377-1384(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP INTERACTION WITH CSM1.
RX PubMed=15023545; DOI=10.1016/j.yexcr.2003.12.008;
RA Wysocka M., Rytka J., Kurlandzka A.;
RT "Saccharomyces cerevisiae CSM1 gene encoding a protein influencing
RT chromosome segregation in meiosis I interacts with elements of the DNA
RT replication complex.";
RL Exp. Cell Res. 294:592-602(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [9]
RP FUNCTION, AND INTERACTION WITH CSM1.
RX PubMed=18997772; DOI=10.1038/nature07460;
RA Mekhail K., Seebacher J., Gygi S.P., Moazed D.;
RT "Role for perinuclear chromosome tethering in maintenance of genome
RT stability.";
RL Nature 456:667-670(2008).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417 AND SER-474, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Member of a perinuclear network that controls recombination
CC at multiple loci to maintain genome stability. Required for rDNA repeat
CC stability. {ECO:0000269|PubMed:18997772}.
CC -!- SUBUNIT: Interacts with CSM1. {ECO:0000269|PubMed:15023545,
CC ECO:0000269|PubMed:18997772}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000269|PubMed:14562095};
CC Multi-pass membrane protein {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 606 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the NUR1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X95644; CAA64922.1; -; Genomic_DNA.
DR EMBL; Z74137; CAA98655.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11769.1; -; Genomic_DNA.
DR PIR; S67625; S67625.
DR RefSeq; NP_010194.1; NM_001180148.1.
DR AlphaFoldDB; Q12066; -.
DR BioGRID; 31971; 65.
DR DIP; DIP-1799N; -.
DR IntAct; Q12066; 9.
DR MINT; Q12066; -.
DR STRING; 4932.YDL089W; -.
DR iPTMnet; Q12066; -.
DR MaxQB; Q12066; -.
DR PaxDb; Q12066; -.
DR PRIDE; Q12066; -.
DR EnsemblFungi; YDL089W_mRNA; YDL089W; YDL089W.
DR GeneID; 851469; -.
DR KEGG; sce:YDL089W; -.
DR SGD; S000002247; NUR1.
DR VEuPathDB; FungiDB:YDL089W; -.
DR eggNOG; ENOG502S7S0; Eukaryota.
DR HOGENOM; CLU_033252_1_0_1; -.
DR InParanoid; Q12066; -.
DR OMA; IKPNYHK; -.
DR BioCyc; YEAST:G3O-29497-MON; -.
DR PRO; PR:Q12066; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q12066; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0034399; C:nuclear periphery; HDA:SGD.
DR GO; GO:0043007; P:maintenance of rDNA; IMP:SGD.
DR GO; GO:0007096; P:regulation of exit from mitosis; IMP:SGD.
DR InterPro; IPR018819; Nur1/Mug154.
DR PANTHER; PTHR28293; PTHR28293; 1.
DR Pfam; PF10332; DUF2418; 1.
PE 1: Evidence at protein level;
KW Membrane; Nucleus; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..484
FT /note="Nuclear rim protein 1"
FT /id="PRO_0000202586"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 416..457
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 430..446
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 3
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 417
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 474
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 484 AA; 56887 MW; 3785895EA2887E89 CRC64;
MGSNDLINEA YDDSEVVGEE RESKSAWMKR WYQLLTSPLD LQLVINEKLE MINWDAYAKS
LAKPLGNFLT ILFFIIRLLQ DNLIKPNYYK LNVKSGAFDL SKSNKLKEFD YLWEISSSFQ
NNNQFYAFQS WYFVTLRFLN NLFRFTIFIL LSLNLYVSCK FMFGYFKTYN LFHLKKEFNS
PNLTKHNLKD LSKEYYEDIY KQSLWSMLKH FFRGSRDDGP HVNQNEDEIF FQLRKWIPTN
FMINLFVSFS PTAIVFLSFS DVSFTSAIAI VFHQYILDYI ITKRFQRSVD DDLILSSAAL
QEYEDKHIMA RINQCSNIDT LSSAMGTRSK TPRIFTTHSL CGEEIREVYN YEKREFEALP
KMTESVPGSR ETRIKDYGGI SQVSDHQSHP IGFHYSPRMS PYYRDKVLDN NLAQSSSNEN
LEKGGAYLPN QDQNRPSKSL SPLRKTPLSA RQKRFEGSEF NVLNKNDINS ILRSPKKKKN
YHKR
