NURA_PYRFU
ID NURA_PYRFU Reviewed; 451 AA.
AC Q8U1N8;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=DNA double-strand break repair nuclease NurA {ECO:0000305};
DE EC=3.1.-.- {ECO:0000269|PubMed:22064858};
GN Name=nurA {ECO:0000303|PubMed:18957200};
GN OrderedLocusNames=PF1168 {ECO:0000312|EMBL:AAL81292.1};
OS Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=186497;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA DiRuggiero J., Robb F.T.;
RT "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT horikoshii inferred from complete genomic sequences.";
RL Genetics 152:1299-1305(1999).
RN [2]
RP FUNCTION, ACTIVITY REGULATION, AND INTERACTION WITH HERA.
RX PubMed=18957200; DOI=10.1016/j.cell.2008.09.054;
RA Hopkins B.B., Paull T.T.;
RT "The P. furiosus mre11/rad50 complex promotes 5' strand resection at a DNA
RT double-strand break.";
RL Cell 135:250-260(2008).
RN [3] {ECO:0007744|PDB:3TAI, ECO:0007744|PDB:3TAL, ECO:0007744|PDB:3TAZ}
RP X-RAY CRYSTALLOGRAPHY (2.82 ANGSTROMS) IN COMPLEX WITH MANGANESE, FUNCTION,
RP COFACTOR, SUBUNIT, AND MUTAGENESIS OF GLU-105 AND HIS-411.
RX PubMed=22064858; DOI=10.1093/nar/gkr999;
RA Chae J., Kim Y.C., Cho Y.;
RT "Crystal structure of the NurA-dAMP-Mn2+ complex.";
RL Nucleic Acids Res. 40:2258-2270(2012).
CC -!- FUNCTION: Involved in DNA double-strand break (DSB) repair
CC (PubMed:18957200). Acts probably with HerA to stimulate resection of
CC the 5' strand and produce the long 3' single-strand that is required
CC for RadA loading (PubMed:18957200). Exhibits 5' endonuclease activity
CC and both 5' and 3' exonuclease activities (PubMed:22064858).
CC {ECO:0000269|PubMed:18957200, ECO:0000269|PubMed:22064858}.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000269|PubMed:22064858};
CC -!- ACTIVITY REGULATION: Exonuclease activity is stimulated in the presence
CC of HerA. {ECO:0000269|PubMed:18957200}.
CC -!- SUBUNIT: Homodimer (PubMed:22064858). Interacts with HerA
CC (PubMed:18957200). {ECO:0000269|PubMed:18957200,
CC ECO:0000269|PubMed:22064858}.
CC -!- SIMILARITY: Belongs to the NurA family. {ECO:0000305}.
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DR EMBL; AE009950; AAL81292.1; -; Genomic_DNA.
DR RefSeq; WP_011012308.1; NC_003413.1.
DR PDB; 3TAI; X-ray; 2.82 A; A/B=1-451.
DR PDB; 3TAL; X-ray; 3.15 A; A/B=1-451.
DR PDB; 3TAZ; X-ray; 3.20 A; A/B=1-451.
DR PDBsum; 3TAI; -.
DR PDBsum; 3TAL; -.
DR PDBsum; 3TAZ; -.
DR AlphaFoldDB; Q8U1N8; -.
DR SMR; Q8U1N8; -.
DR STRING; 186497.PF1168; -.
DR PRIDE; Q8U1N8; -.
DR EnsemblBacteria; AAL81292; AAL81292; PF1168.
DR GeneID; 1469037; -.
DR KEGG; pfu:PF1168; -.
DR PATRIC; fig|186497.12.peg.1228; -.
DR eggNOG; arCOG00367; Archaea.
DR HOGENOM; CLU_665023_0_0_2; -.
DR OMA; LVYTNAM; -.
DR OrthoDB; 45596at2157; -.
DR PhylomeDB; Q8U1N8; -.
DR Proteomes; UP000001013; Chromosome.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR InterPro; IPR016738; NurA-like.
DR InterPro; IPR018977; NurA_domain.
DR Pfam; PF09376; NurA; 1.
DR PIRSF; PIRSF018871; UCP018871; 1.
DR SMART; SM00933; NurA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA damage; DNA repair; Endonuclease; Exonuclease; Hydrolase;
KW Manganese; Metal-binding; Nuclease; Reference proteome.
FT CHAIN 1..451
FT /note="DNA double-strand break repair nuclease NurA"
FT /id="PRO_0000434027"
FT BINDING 51
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000269|PubMed:22064858,
FT ECO:0007744|PDB:3TAL, ECO:0007744|PDB:3TAZ"
FT BINDING 126
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000269|PubMed:22064858,
FT ECO:0007744|PDB:3TAL, ECO:0007744|PDB:3TAZ"
FT MUTAGEN 105
FT /note="E->A: Lack of endonuclease activity. Weak 5'
FT exonuclease activity, but efficient 3' exo- and 5'
FT endonuclease activities in the presence of HerA."
FT /evidence="ECO:0000269|PubMed:22064858"
FT MUTAGEN 411
FT /note="H->A: Lack of nuclease activity. Weak activity in
FT the presence of HerA."
FT /evidence="ECO:0000269|PubMed:22064858"
FT HELIX 6..23
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 29..34
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 46..59
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 62..75
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 78..87
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 90..114
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 118..127
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 131..134
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 141..153
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 155..183
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 184..187
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 192..203
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 205..207
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 208..210
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 212..219
FT /evidence="ECO:0007829|PDB:3TAI"
FT TURN 223..226
FT /evidence="ECO:0007829|PDB:3TAL"
FT STRAND 231..233
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 235..239
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 242..244
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 245..253
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 258..262
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 263..286
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 290..297
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 302..307
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 315..325
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 332..335
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 344..346
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 349..352
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 356..363
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 366..372
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 374..377
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 380..386
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 388..396
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 401..404
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 405..415
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 421..428
FT /evidence="ECO:0007829|PDB:3TAI"
FT STRAND 431..433
FT /evidence="ECO:0007829|PDB:3TAI"
FT HELIX 437..439
FT /evidence="ECO:0007829|PDB:3TAI"
SQ SEQUENCE 451 AA; 51609 MW; 80304F73B7F44A61 CRC64;
MRLLSKQSIE RITKILLDEL ENVRENEQIR NIINSWKPLP SPEKSSIYAV DGSRSVSRLS
GTVIYFLSAL AVGSGKQLRL SYANAIKSNY GTSDQIVRMQ METLENMLGY LAYRKLEGEK
RAILMDGTLT GSLVRPPVYP EDIRSLNVMR ALIGESDFEN LLNEFLEKLR DHYRKVEEHL
EKNGNYDSPI LTDNVVEKLR KKYIDTKVIA YGSGKVKVKI PRKALGYSPR VIPIEVLESS
RGKSVDELLQ ELDEEKVELY LGKDDIYDAL HMTLSYIEYL YSIDKLLEVK NLAYIAKSFY
TKTLARTLGV EIVDTALLDA VIRTLIGHEK EGYLEIEHAV VPPKWSFPDF LLSKFRNIEK
LIDKGIHLAY VRFEQGDVIY MLQSTTNIEK ILPLILHHKA GGYLRPLQLA HHGVKISYKE
ARHTLEALIN ALRNRDPALK IFVKYGRSPL E
