NURA_SACS2
ID NURA_SACS2 Reviewed; 339 AA.
AC Q97WH1;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=DNA double-strand break repair nuclease NurA {ECO:0000305};
DE EC=3.1.-.- {ECO:0000305};
GN Name=nurA {ECO:0000303|PubMed:22135300};
GN OrderedLocusNames=SSO2248 {ECO:0000312|EMBL:AAK42416.1};
OS Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS (Sulfolobus solfataricus).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Saccharolobus.
OX NCBI_TaxID=273057;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=11427726; DOI=10.1073/pnas.141222098;
RA She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN [2]
RP SUBUNIT, AND INTERACTION WITH HERA.
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=25447518; DOI=10.1016/j.febslet.2014.10.035;
RA Byrne R.T., Schuller J.M., Unverdorben P., Foerster F., Hopfner K.P.;
RT "Molecular architecture of the HerA-NurA DNA double-strand break resection
RT complex.";
RL FEBS Lett. 588:4637-4644(2014).
RN [3] {ECO:0007744|PDB:2YGK}
RP X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS), FUNCTION, ACTIVITY REGULATION,
RP SUBUNIT, INTERACTION WITH HERA, AND MUTAGENESIS OF LYS-202; ILE-295 AND
RP PHE-300.
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=22135300; DOI=10.1093/nar/gkr1157;
RA Blackwood J.K., Rzechorzek N.J., Abrams A.S., Maman J.D., Pellegrini L.,
RA Robinson N.P.;
RT "Structural and functional insights into DNA-end processing by the archaeal
RT HerA helicase-NurA nuclease complex.";
RL Nucleic Acids Res. 40:3183-3196(2012).
CC -!- FUNCTION: Involved in DNA double-strand break (DSB) repair
CC (PubMed:22135300). Acts probably with HerA to stimulate resection of
CC the 5' strand and produce the long 3' single-strand that is required
CC for RadA loading (By similarity). Processes linear dsDNA probes with 3'
CC or 5' single-stranded overhangs or blunt ends (PubMed:22135300).
CC {ECO:0000250|UniProtKB:Q8U1N8, ECO:0000269|PubMed:22135300}.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000250|UniProtKB:Q8U1N8};
CC -!- ACTIVITY REGULATION: Nuclease activity requires the presence of HerA.
CC {ECO:0000269|PubMed:22135300}.
CC -!- SUBUNIT: Homodimer (PubMed:22135300, PubMed:25447518). Forms a complex
CC with HerA (PubMed:22135300, PubMed:25447518).
CC {ECO:0000269|PubMed:22135300, ECO:0000269|PubMed:25447518}.
CC -!- INTERACTION:
CC Q97WH1; Q97WG8: herA; NbExp=3; IntAct=EBI-10110462, EBI-10110451;
CC -!- SIMILARITY: Belongs to the NurA family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE006641; AAK42416.1; -; Genomic_DNA.
DR PIR; A90395; A90395.
DR RefSeq; WP_009991544.1; NC_002754.1.
DR PDB; 2YGK; X-ray; 2.50 A; A/B=1-339.
DR PDBsum; 2YGK; -.
DR AlphaFoldDB; Q97WH1; -.
DR SMR; Q97WH1; -.
DR IntAct; Q97WH1; 1.
DR MINT; Q97WH1; -.
DR STRING; 273057.SSO2248; -.
DR EnsemblBacteria; AAK42416; AAK42416; SSO2248.
DR GeneID; 44127982; -.
DR KEGG; sso:SSO2248; -.
DR PATRIC; fig|273057.12.peg.2343; -.
DR eggNOG; arCOG00367; Archaea.
DR HOGENOM; CLU_835811_0_0_2; -.
DR InParanoid; Q97WH1; -.
DR OMA; YILKKSH; -.
DR Proteomes; UP000001974; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR InterPro; IPR018977; NurA_domain.
DR Pfam; PF09376; NurA; 1.
DR SMART; SM00933; NurA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA damage; DNA repair; Hydrolase; Manganese; Metal-binding;
KW Nuclease; Reference proteome.
FT CHAIN 1..339
FT /note="DNA double-strand break repair nuclease NurA"
FT /id="PRO_0000434029"
FT BINDING 58
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:Q8U1N8"
FT BINDING 133
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:Q8U1N8"
FT MUTAGEN 202
FT /note="K->A: Interacts with HerA but lacks activity."
FT /evidence="ECO:0000269|PubMed:22135300"
FT MUTAGEN 295
FT /note="I->E: Does not interact with HerA. Does not affect
FT dimerization. Strong decrease in nucleolytic activity."
FT /evidence="ECO:0000269|PubMed:22135300"
FT MUTAGEN 300
FT /note="F->E: Decreases interaction with HerA. Does not
FT affect dimerization. Decreases nucleolytic activity."
FT /evidence="ECO:0000269|PubMed:22135300"
FT HELIX 3..9
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 17..38
FT /evidence="ECO:0007829|PDB:2YGK"
FT STRAND 39..41
FT /evidence="ECO:0007829|PDB:2YGK"
FT STRAND 54..65
FT /evidence="ECO:0007829|PDB:2YGK"
FT STRAND 70..82
FT /evidence="ECO:0007829|PDB:2YGK"
FT STRAND 85..98
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 104..123
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 124..126
FT /evidence="ECO:0007829|PDB:2YGK"
FT STRAND 127..134
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 136..140
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 151..159
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 162..166
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 171..193
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 194..196
FT /evidence="ECO:0007829|PDB:2YGK"
FT STRAND 197..202
FT /evidence="ECO:0007829|PDB:2YGK"
FT TURN 208..210
FT /evidence="ECO:0007829|PDB:2YGK"
FT STRAND 212..214
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 216..223
FT /evidence="ECO:0007829|PDB:2YGK"
FT STRAND 226..230
FT /evidence="ECO:0007829|PDB:2YGK"
FT STRAND 233..236
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 248..251
FT /evidence="ECO:0007829|PDB:2YGK"
FT STRAND 255..264
FT /evidence="ECO:0007829|PDB:2YGK"
FT STRAND 270..276
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 280..291
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 300..309
FT /evidence="ECO:0007829|PDB:2YGK"
FT HELIX 313..330
FT /evidence="ECO:0007829|PDB:2YGK"
SQ SEQUENCE 339 AA; 39125 MW; ACF81EEF43C015A8 CRC64;
MIRKIYDKLV ESHNEIKNQI YNIANYLKQE IQDKVNEYWN EYVINHEQSE TCKFVAIDGG
SFGRPMRIGI VYAVGAESVI GDNKGVKTLS EDGQIGIFKP GNDAQERISL LMEALELSLA
LRDGSKGDYI LMDGSLSKKI GNKVDIQQFS DEELKLIRNV DLNGIISIKD ERKMRDLLML
LNQFLVSKII EEYDGNVLWI SKVSRGRDLF GTDYPDITVL ELFTEKRGFS KLIIKNIDIE
KISEIPEIEV LRKMEYTTFY TRLDNGKRVI RVDIVGRVDE KIVKEIMDRL SGVSIKGYPF
PLLKAHMDVR FSAMDREKII KLVGSKLHKD IEWWPSQFY
