ID   NURA_SULAC              Reviewed;         332 AA.
AC   F2Z6F6; Q4JCJ9; Q8NKP9;
DT   14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=DNA double-strand break repair nuclease NurA {ECO:0000305};
DE            EC=3.1.-.- {ECO:0000269|PubMed:12052775};
DE   AltName: Full=Nuclease repair of Archaea {ECO:0000303|PubMed:12052775};
GN   Name=nurA {ECO:0000303|PubMed:12052775};
GN   Synonyms=p39 {ECO:0000303|PubMed:12052775};
GN   OrderedLocusNames=Saci_0050 {ECO:0000312|EMBL:AAY79480.1};
OS   Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC
OS   15157 / NCIMB 11770).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Sulfolobus.
OX   NCBI_TaxID=330779;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A NUCLEASE, AND COFACTOR.
RC   STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX   PubMed=12052775; DOI=10.1093/embo-reports/kvf112;
RA   Constantinesco F., Forterre P., Elie C.;
RT   "NurA, a novel 5'-3' nuclease gene linked to rad50 and mre11 homologs of
RT   thermophilic Archaea.";
RL   EMBO Rep. 3:537-542(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX   PubMed=15995215; DOI=10.1128/jb.187.14.4992-4999.2005;
RA   Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E.,
RA   Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.;
RT   "The genome of Sulfolobus acidocaldarius, a model organism of the
RT   Crenarchaeota.";
RL   J. Bacteriol. 187:4992-4999(2005).
CC   -!- FUNCTION: Involved in DNA double-strand break (DSB) repair
CC       (PubMed:12052775). Acts probably with HerA to stimulate resection of
CC       the 5' strand and produce the long 3' single-strand that is required
CC       for RadA loading (By similarity). Exhibits both single-stranded
CC       endonuclease activity and 5'-3' exonuclease activity on single-stranded
CC       and double-stranded DNA (PubMed:12052775).
CC       {ECO:0000250|UniProtKB:Q8U1N8, ECO:0000269|PubMed:12052775}.
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000269|PubMed:12052775};
CC   -!- SIMILARITY: Belongs to the NurA family. {ECO:0000305}.
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DR   EMBL; AJ437617; CAD26846.1; -; Genomic_DNA.
DR   EMBL; CP000077; AAY79480.1; -; Genomic_DNA.
DR   RefSeq; WP_011276981.1; NC_007181.1.
DR   AlphaFoldDB; F2Z6F6; -.
DR   SMR; F2Z6F6; -.
DR   STRING; 330779.Saci_0050; -.
DR   EnsemblBacteria; AAY79480; AAY79480; Saci_0050.
DR   GeneID; 3472965; -.
DR   KEGG; sai:Saci_0050; -.
DR   PATRIC; fig|330779.12.peg.47; -.
DR   eggNOG; arCOG00367; Archaea.
DR   HOGENOM; CLU_835811_0_0_2; -.
DR   OMA; YILKKSH; -.
DR   Proteomes; UP000001018; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   InterPro; IPR018977; NurA_domain.
DR   Pfam; PF09376; NurA; 1.
DR   SMART; SM00933; NurA; 1.
PE   1: Evidence at protein level;
KW   DNA damage; DNA repair; Endonuclease; Exonuclease; Hydrolase; Manganese;
KW   Metal-binding; Nuclease; Reference proteome.
FT   CHAIN           1..332
FT                   /note="DNA double-strand break repair nuclease NurA"
FT                   /id="PRO_0000434028"
FT   BINDING         57
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250|UniProtKB:Q8U1N8"
FT   BINDING         132
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250|UniProtKB:Q8U1N8"
SQ   SEQUENCE   332 AA;  38888 MW;  645A015E2B534C92 CRC64;
     MIDKAYEELI KMKEKIMRDS YTIHKELSES VESILNELWI NYSPESVEHS KKLLAIDGGM
     WVKETRQGVI FIVNAKAIVF EGINEINSEG KVLVHIFSPG NYAKERIELL MQLLELQLAL
     KLVENVDYVL LDGSFSKKLG RHKSELKVDL LDDIVSIDKI LSLEEKDEDN MLRFLIAENQ
     LVLSELVSRY KDKLLFISKN SKSSDLFKQA YSDITILELF TQNCGYSKIL EKKIDENYIL
     SRKASKLLSG LNYYFTNLRL EPSERLFRLD FFNADKIFEY LKVLKPVSLK GYPYPLIKVH
     KDVRVGKEDR ERIYSILEMK RKDISWWPSQ FY