NUSA_BACSU
ID NUSA_BACSU Reviewed; 371 AA.
AC P32727; O31756;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN Name=nusA {ECO:0000255|HAMAP-Rule:MF_00945}; OrderedLocusNames=BSU16600;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8491709; DOI=10.1128/jb.175.10.2880-2887.1993;
RA Shazand K., Tucker J., Grunberg-Manago M., Rabinowitz J.C., Leighton T.;
RT "Similar organization of the nusA-infB operon in Bacillus subtilis and
RT Escherichia coli.";
RL J. Bacteriol. 175:2880-2887(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: Participates in both transcription termination and
CC antitermination. {ECO:0000255|HAMAP-Rule:MF_00945}.
CC -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC dependent RNA polymerase and to nascent RNA. {ECO:0000255|HAMAP-
CC Rule:MF_00945}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945}.
CC -!- SIMILARITY: Belongs to the NusA family. {ECO:0000255|HAMAP-
CC Rule:MF_00945}.
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DR EMBL; Z18631; CAA79231.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13533.1; -; Genomic_DNA.
DR PIR; C36905; C36905.
DR RefSeq; NP_389542.1; NC_000964.3.
DR RefSeq; WP_003231912.1; NZ_JNCM01000035.1.
DR PDB; 2MT4; NMR; -; A=1-124.
DR PDBsum; 2MT4; -.
DR AlphaFoldDB; P32727; -.
DR BMRB; P32727; -.
DR SMR; P32727; -.
DR IntAct; P32727; 1.
DR MINT; P32727; -.
DR STRING; 224308.BSU16600; -.
DR jPOST; P32727; -.
DR PaxDb; P32727; -.
DR PRIDE; P32727; -.
DR EnsemblBacteria; CAB13533; CAB13533; BSU_16600.
DR GeneID; 939628; -.
DR KEGG; bsu:BSU16600; -.
DR PATRIC; fig|224308.179.peg.1801; -.
DR eggNOG; COG0195; Bacteria.
DR InParanoid; P32727; -.
DR OMA; MDIDMSA; -.
DR PhylomeDB; P32727; -.
DR BioCyc; BSUB:BSU16600-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR GO; GO:0031564; P:transcription antitermination; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.1480.10; -; 1.
DR Gene3D; 3.30.300.20; -; 2.
DR HAMAP; MF_00945_B; NusA_B; 1.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR025249; KH_dom_NusA-like.
DR InterPro; IPR009019; KH_sf_prok-type.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR030842; NusA_bac.
DR InterPro; IPR036555; NusA_N_sf.
DR InterPro; IPR022967; S1_dom.
DR InterPro; IPR003029; S1_domain.
DR InterPro; IPR013735; TF_NusA_N.
DR InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR PANTHER; PTHR22648; PTHR22648; 1.
DR Pfam; PF13184; KH_5; 1.
DR Pfam; PF08529; NusA_N; 1.
DR Pfam; PF00575; S1; 1.
DR SMART; SM00322; KH; 2.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF54814; SSF54814; 2.
DR SUPFAM; SSF69705; SSF69705; 1.
DR TIGRFAMs; TIGR01953; NusA; 1.
DR PROSITE; PS50084; KH_TYPE_1; 1.
DR PROSITE; PS50126; S1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome; RNA-binding; Transcription;
KW Transcription antitermination; Transcription regulation;
KW Transcription termination.
FT CHAIN 1..371
FT /note="Transcription termination/antitermination protein
FT NusA"
FT /id="PRO_0000181960"
FT DOMAIN 135..199
FT /note="S1 motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT DOMAIN 301..367
FT /note="KH"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT REGION 347..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 356..371
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 111..112
FT /note="KQ -> NE (in Ref. 1; CAA79231)"
FT /evidence="ECO:0000305"
FT HELIX 4..13
FT /evidence="ECO:0007829|PDB:2MT4"
FT HELIX 20..36
FT /evidence="ECO:0007829|PDB:2MT4"
FT TURN 40..42
FT /evidence="ECO:0007829|PDB:2MT4"
FT STRAND 46..50
FT /evidence="ECO:0007829|PDB:2MT4"
FT TURN 51..54
FT /evidence="ECO:0007829|PDB:2MT4"
FT STRAND 55..67
FT /evidence="ECO:0007829|PDB:2MT4"
FT HELIX 77..81
FT /evidence="ECO:0007829|PDB:2MT4"
FT STRAND 92..96
FT /evidence="ECO:0007829|PDB:2MT4"
FT HELIX 102..123
FT /evidence="ECO:0007829|PDB:2MT4"
SQ SEQUENCE 371 AA; 41725 MW; 2FC7861E4C3094D3 CRC64;
MSSELLDALT ILEKEKGISK EIIIEAIEAA LISAYKRNFN QAQNVRVDLN RETGSIRVFA
RKDVVDEVYD QRLEISIEEA QGIHPEYMVG DVVEIEVTPK DFGRIAAQTA KQVVTQRVRE
AERGVIYSEF IDREEDIMTG IVQRLDNKFI YVSLGKIEAL LPVNEQMPNE SYKPHDRIKV
YITKVEKTTK GPQIYVSRTH PGLLKRLFEI EVPEIYDGTV ELKSVAREAG DRSKISVRTD
DPDVDPVGSC VGPKGQRVQA IVNELKGEKI DIVNWSSDPV EFVANALSPS KVLDVIVNEE
EKATTVIVPD YQLSLAIGKR GQNARLAAKL TGWKIDIKSE TDARELGIYP RELEEDDEPL
FTEPETAESD E
