ID   NUSA_BUCAP              Reviewed;         495 AA.
AC   Q8K9H0;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN   Name=nusA {ECO:0000255|HAMAP-Rule:MF_00945}; OrderedLocusNames=BUsg_366;
OS   Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=198804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sg;
RX   PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Participates in both transcription termination and
CC       antitermination. {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC       dependent RNA polymerase and to nascent RNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- SIMILARITY: Belongs to the NusA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
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DR   EMBL; AE013218; AAM67919.1; -; Genomic_DNA.
DR   RefSeq; WP_011053886.1; NC_004061.1.
DR   AlphaFoldDB; Q8K9H0; -.
DR   SMR; Q8K9H0; -.
DR   STRING; 198804.BUsg_366; -.
DR   EnsemblBacteria; AAM67919; AAM67919; BUsg_366.
DR   KEGG; bas:BUsg_366; -.
DR   eggNOG; COG0195; Bacteria.
DR   HOGENOM; CLU_029242_0_0_6; -.
DR   OMA; MDIDMSA; -.
DR   OrthoDB; 384865at2; -.
DR   Proteomes; UP000000416; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR   GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.1480.10; -; 1.
DR   Gene3D; 3.30.300.20; -; 2.
DR   HAMAP; MF_00945_B; NusA_B; 1.
DR   InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR015946; KH_dom-like_a/b.
DR   InterPro; IPR025249; KH_dom_NusA-like.
DR   InterPro; IPR009019; KH_sf_prok-type.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR030842; NusA_bac.
DR   InterPro; IPR036555; NusA_N_sf.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   InterPro; IPR013735; TF_NusA_N.
DR   InterPro; IPR010214; Tscrpt_termin_fac_NusA_C_rpt.
DR   InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR   PANTHER; PTHR22648; PTHR22648; 1.
DR   Pfam; PF13184; KH_5; 1.
DR   Pfam; PF08529; NusA_N; 1.
DR   SMART; SM00322; KH; 2.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF47794; SSF47794; 2.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF54814; SSF54814; 2.
DR   SUPFAM; SSF69705; SSF69705; 1.
DR   TIGRFAMs; TIGR01953; NusA; 1.
DR   TIGRFAMs; TIGR01954; nusA_Cterm_rpt; 2.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
DR   PROSITE; PS50126; S1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Repeat; RNA-binding; Transcription;
KW   Transcription antitermination; Transcription regulation;
KW   Transcription termination.
FT   CHAIN           1..495
FT                   /note="Transcription termination/antitermination protein
FT                   NusA"
FT                   /id="PRO_0000181963"
FT   DOMAIN          135..200
FT                   /note="S1 motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   DOMAIN          302..370
FT                   /note="KH"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   REPEAT          364..414
FT                   /note="1"
FT   REPEAT          439..489
FT                   /note="2"
FT   REGION          364..489
FT                   /note="2 X 51 AA approximate repeats"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
SQ   SEQUENCE   495 AA;  55643 MW;  75F1F5C22C586027 CRC64;
     MNKEILAVVE AVSNEKSLPR EKIFEALEIA LATATKKKYE QEIDVRVSIN RKTGNFSTFR
     RWMVVDIVVH PTKEITLEAA CFEGEQVKIN DYVEDKIESV NFDRITTQTA KQVIVQKVRE
     AERAMLVDQF RKHIGQIITG IVKKISRDNL TLDLGNNAEA LILREGMLPR ENFRPGDRIR
     GILYGVYPEA RGAQLFLSRS KTEMLIELFR IEVPEIGEEV IEIKAAARDP GSRAKIAVKT
     NDKRIDPIGA CVGMRGARVQ AVSSELCGER IDIILWDDNP AQFVINAMAP ADVVSIIVDE
     DHHTMDIAVD SSNLAQAIGR NGQNVRLASQ ISGWELNVMT TEDLRSKHKE EAYAAFNIFK
     KNLNVSEKVI KTLVKEGFSS LEELAYIPLN ELLEINNLTE REVQLVREGA KNGLLLLELD
     QKNQIKNKKI EQALLNVNGM NELLALKLAE KNIFTVEELA NQGIDDLIDI ENLNSEQAGL
     LIMAARNICW FSSKV