ID   NUSA_BUCBP              Reviewed;         497 AA.
AC   Q89AF4;
DT   30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2003, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN   Name=nusA {ECO:0000255|HAMAP-Rule:MF_00945}; OrderedLocusNames=bbp_341;
OS   Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=224915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bp;
RX   PubMed=12522265; DOI=10.1073/pnas.0235981100;
RA   van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F.,
RA   Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J.,
RA   Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.;
RT   "Reductive genome evolution in Buchnera aphidicola.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003).
CC   -!- FUNCTION: Participates in both transcription termination and
CC       antitermination. {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC       dependent RNA polymerase and to nascent RNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- SIMILARITY: Belongs to the NusA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
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DR   EMBL; AE016826; AAO27062.1; -; Genomic_DNA.
DR   RefSeq; WP_011091463.1; NC_004545.1.
DR   AlphaFoldDB; Q89AF4; -.
DR   SMR; Q89AF4; -.
DR   STRING; 224915.bbp_341; -.
DR   EnsemblBacteria; AAO27062; AAO27062; bbp_341.
DR   GeneID; 56470880; -.
DR   KEGG; bab:bbp_341; -.
DR   eggNOG; COG0195; Bacteria.
DR   HOGENOM; CLU_029242_0_0_6; -.
DR   OMA; MDIDMSA; -.
DR   OrthoDB; 384865at2; -.
DR   Proteomes; UP000000601; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR   GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.1480.10; -; 1.
DR   Gene3D; 3.30.300.20; -; 2.
DR   HAMAP; MF_00945_B; NusA_B; 1.
DR   InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR015946; KH_dom-like_a/b.
DR   InterPro; IPR025249; KH_dom_NusA-like.
DR   InterPro; IPR009019; KH_sf_prok-type.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR030842; NusA_bac.
DR   InterPro; IPR036555; NusA_N_sf.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   InterPro; IPR013735; TF_NusA_N.
DR   InterPro; IPR010214; Tscrpt_termin_fac_NusA_C_rpt.
DR   InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR   PANTHER; PTHR22648; PTHR22648; 1.
DR   Pfam; PF13184; KH_5; 1.
DR   Pfam; PF08529; NusA_N; 1.
DR   SMART; SM00322; KH; 2.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF47794; SSF47794; 2.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF54814; SSF54814; 2.
DR   SUPFAM; SSF69705; SSF69705; 1.
DR   TIGRFAMs; TIGR01953; NusA; 1.
DR   TIGRFAMs; TIGR01954; nusA_Cterm_rpt; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
DR   PROSITE; PS50126; S1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; Repeat; RNA-binding; Transcription;
KW   Transcription antitermination; Transcription regulation;
KW   Transcription termination.
FT   CHAIN           1..497
FT                   /note="Transcription termination/antitermination protein
FT                   NusA"
FT                   /id="PRO_0000181964"
FT   DOMAIN          135..200
FT                   /note="S1 motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   DOMAIN          302..372
FT                   /note="KH"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   REPEAT          364..414
FT                   /note="1"
FT   REPEAT          439..489
FT                   /note="2"
FT   REGION          364..489
FT                   /note="2 X 51 AA approximate repeats"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
SQ   SEQUENCE   497 AA;  56245 MW;  089FB45F87B8FF90 CRC64;
     MNKEILAVVE AVSNEKSLPR EKIFEALECA LAIATKKKYD QEIDVRVKIN RKNGNFETFR
     RWLVVNQVLQ PTKEITLEAA KFEDVTVKVN DYIEDKMISV TFDRITTQTA KQVIVQKVRE
     AERAMIVEQF RKYFGKILTG IVKKINRESI TLDLGNNAEA LILKEDMLPR ENFRLGDRVR
     GVLYAIYPES RGAQLFVTRS KPEMLIELFK IEVPEIGEEI IEIKSAARDP GSRAKIAVKS
     NDKRVDAVGA CVGMRGARVQ AVSSELCGER IDIVLWDKNS AQFIINSMAP AEVSSIVLDE
     DRHTIDIAVD SNNLAQAIGR NGQNVRLASQ LSGWEINVMT VDDLKLKHQE EKDKILNIFT
     KYLKIDQKIS NILIDEGFSS IEELVYIPFN ELLNINSITQ ELAYSIRESA KKALCAIELE
     NKKVINERKL HKDLLNLKGM NQKLAFKLAK KNIFSLEDLA EQGIDDLIDI ENLNSNTAGM
     LIMAARNICW FGNKTSA