ID   NUSA_COXBU              Reviewed;         503 AA.
AC   Q83BS0;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN   Name=nusA {ECO:0000255|HAMAP-Rule:MF_00945}; OrderedLocusNames=CBU_1433;
OS   Coxiella burnetii (strain RSA 493 / Nine Mile phase I).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae;
OC   Coxiella.
OX   NCBI_TaxID=227377;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RSA 493 / Nine Mile phase I;
RX   PubMed=12704232; DOI=10.1073/pnas.0931379100;
RA   Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C.,
RA   Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T.,
RA   Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M.,
RA   Lee K.H., Carty H.A., Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E.,
RA   Fraser C.M., Heidelberg J.F.;
RT   "Complete genome sequence of the Q-fever pathogen, Coxiella burnetii.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND DEVELOPMENTAL STAGE.
RC   STRAIN=Nine Mile Crazy / RSA 514;
RX   PubMed=17088354; DOI=10.1128/iai.00883-06;
RA   Coleman S.A., Fischer E.R., Cockrell D.C., Voth D.E., Howe D., Mead D.J.,
RA   Samuel J.E., Heinzen R.A.;
RT   "Proteome and antigen profiling of Coxiella burnetii developmental forms.";
RL   Infect. Immun. 75:290-298(2007).
CC   -!- FUNCTION: Participates in both transcription termination and
CC       antitermination. {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC       dependent RNA polymerase and to nascent RNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- DEVELOPMENTAL STAGE: More than twofold more abundant in the large cell
CC       variant (LCV) stage than in the small cell variant (SCV) stage (at
CC       protein level). LCVs are more metabolically active than SCVs.
CC       {ECO:0000269|PubMed:17088354}.
CC   -!- SIMILARITY: Belongs to the NusA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
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DR   EMBL; AE016828; AAO90930.1; -; Genomic_DNA.
DR   RefSeq; NP_820416.1; NC_002971.3.
DR   RefSeq; WP_010958224.1; NC_002971.4.
DR   AlphaFoldDB; Q83BS0; -.
DR   SMR; Q83BS0; -.
DR   STRING; 227377.CBU_1433; -.
DR   PRIDE; Q83BS0; -.
DR   EnsemblBacteria; AAO90930; AAO90930; CBU_1433.
DR   GeneID; 1209339; -.
DR   KEGG; cbu:CBU_1433; -.
DR   PATRIC; fig|227377.7.peg.1432; -.
DR   eggNOG; COG0195; Bacteria.
DR   eggNOG; COG2251; Bacteria.
DR   HOGENOM; CLU_029242_0_0_6; -.
DR   OMA; MDIDMSA; -.
DR   Proteomes; UP000002671; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR   GO; GO:0031564; P:transcription antitermination; IBA:GO_Central.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.1480.10; -; 1.
DR   Gene3D; 3.30.300.20; -; 2.
DR   HAMAP; MF_00945_B; NusA_B; 1.
DR   InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR015946; KH_dom-like_a/b.
DR   InterPro; IPR025249; KH_dom_NusA-like.
DR   InterPro; IPR009019; KH_sf_prok-type.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR030842; NusA_bac.
DR   InterPro; IPR036555; NusA_N_sf.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   InterPro; IPR013735; TF_NusA_N.
DR   InterPro; IPR010214; Tscrpt_termin_fac_NusA_C_rpt.
DR   InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR   PANTHER; PTHR22648; PTHR22648; 1.
DR   Pfam; PF13184; KH_5; 1.
DR   Pfam; PF08529; NusA_N; 1.
DR   SMART; SM00322; KH; 2.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF47794; SSF47794; 2.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF54814; SSF54814; 2.
DR   SUPFAM; SSF69705; SSF69705; 1.
DR   TIGRFAMs; TIGR01953; NusA; 1.
DR   TIGRFAMs; TIGR01954; nusA_Cterm_rpt; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
DR   PROSITE; PS50126; S1; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Reference proteome; RNA-binding; Transcription;
KW   Transcription antitermination; Transcription regulation;
KW   Transcription termination.
FT   CHAIN           1..503
FT                   /note="Transcription termination/antitermination protein
FT                   NusA"
FT                   /id="PRO_0000322127"
FT   DOMAIN          140..206
FT                   /note="S1 motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   DOMAIN          308..374
FT                   /note="KH"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
SQ   SEQUENCE   503 AA;  56275 MW;  5BC10970CA23964F CRC64;
     MNKDILLIVD SMSNERGVSK EVIFEAIEAA LAAVTAKRYE EDDVKIRVAI DQKTGDYESF
     RCWTVVEDTN ESLEFPNQEM TLKQAREIDS DLEVGDVIEE PVESVKFGRI AVQQAKQVIV
     QKVREAERAK IIRQYEKRVG ELVIGVVKRV TRESIILDMG ENAEALLLRE EMIPREAFRI
     NDRLRAYLYS VCQDKKRGPQ LLVSRTRPEF LVELFKIEVP EIGEEVIEIK GAARDPGSRA
     KIAVKTNDGR IDPIGACVGM RGSRVQAVSN ELGGERIDIV LWDDNPAQLV INAMAPAEVA
     SIVVDEDSHT MDIAVNKDQL SQAIGRSGQN VRLASELTGW TLNVMSEAEM AQKHEKEAGK
     IKTAFMEKLD VDEEVADALV QAGFMNLEEV AYVPKEELQG VEGFDEDISA ELQRRAGDVL
     LTQEIAKQEL DEKKPAEDLL TLPGMTTELA RQLVENEVLT RDDLAEKSVL DLKEIIEIDD
     EAAANLIMAA RAHWFAEEES EKS