NUSA_ECO57
ID NUSA_ECO57 Reviewed; 495 AA.
AC P0AFF8; P03003;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN Name=nusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN OrderedLocusNames=Z4530, ECs4050;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Participates in both transcription termination and
CC antitermination. {ECO:0000255|HAMAP-Rule:MF_00945}.
CC -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC dependent RNA polymerase and to nascent RNA. {ECO:0000255|HAMAP-
CC Rule:MF_00945}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945}.
CC -!- SIMILARITY: Belongs to the NusA family. {ECO:0000255|HAMAP-
CC Rule:MF_00945}.
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DR EMBL; AE005174; AAG58305.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB37473.1; -; Genomic_DNA.
DR PIR; B91135; B91135.
DR PIR; E85980; E85980.
DR RefSeq; NP_312077.1; NC_002695.1.
DR RefSeq; WP_001031057.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0AFF8; -.
DR BMRB; P0AFF8; -.
DR SMR; P0AFF8; -.
DR STRING; 155864.EDL933_4398; -.
DR EnsemblBacteria; AAG58305; AAG58305; Z4530.
DR EnsemblBacteria; BAB37473; BAB37473; ECs_4050.
DR GeneID; 66672929; -.
DR GeneID; 916098; -.
DR KEGG; ece:Z4530; -.
DR KEGG; ecs:ECs_4050; -.
DR PATRIC; fig|386585.9.peg.4229; -.
DR eggNOG; COG0195; Bacteria.
DR HOGENOM; CLU_029242_0_0_6; -.
DR OMA; MDIDMSA; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.1480.10; -; 1.
DR Gene3D; 3.30.300.20; -; 2.
DR HAMAP; MF_00945_B; NusA_B; 1.
DR InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR025249; KH_dom_NusA-like.
DR InterPro; IPR009019; KH_sf_prok-type.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR030842; NusA_bac.
DR InterPro; IPR036555; NusA_N_sf.
DR InterPro; IPR022967; S1_dom.
DR InterPro; IPR003029; S1_domain.
DR InterPro; IPR013735; TF_NusA_N.
DR InterPro; IPR010214; Tscrpt_termin_fac_NusA_C_rpt.
DR InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR PANTHER; PTHR22648; PTHR22648; 1.
DR Pfam; PF13184; KH_5; 1.
DR Pfam; PF08529; NusA_N; 1.
DR Pfam; PF00575; S1; 1.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF47794; SSF47794; 2.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF54814; SSF54814; 2.
DR SUPFAM; SSF69705; SSF69705; 1.
DR TIGRFAMs; TIGR01953; NusA; 1.
DR TIGRFAMs; TIGR01954; nusA_Cterm_rpt; 2.
DR PROSITE; PS50084; KH_TYPE_1; 1.
DR PROSITE; PS50126; S1; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome; Repeat; RNA-binding; Transcription;
KW Transcription antitermination; Transcription regulation;
KW Transcription termination.
FT CHAIN 1..495
FT /note="Transcription termination/antitermination protein
FT NusA"
FT /id="PRO_0000181966"
FT DOMAIN 135..200
FT /note="S1 motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT DOMAIN 302..368
FT /note="KH"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT REPEAT 364..414
FT /note="1"
FT REPEAT 439..489
FT /note="2"
FT REGION 364..489
FT /note="2 X 51 AA approximate repeats"
SQ SEQUENCE 495 AA; 54871 MW; 7D4DD019172FBAD0 CRC64;
MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVQID RKSGDFDTFR
RWLVVDEVTQ PTKEITLEAA RYEDESLNLG DYVEDQIESV TFDRITTQTA KQVIVQKVRE
AERAMVVDQF REHEGEIITG VVKKVNRDNI SLDLGNNAEA VILREDMLPR ENFRPGDRVR
GVLYSVRPEA RGAQLFVTRS KPEMLIELFR IEVPEIGEEV IEIKAAARDP GSRAKIAVKT
NDKRIDPVGA CVGMRGARVQ AVSTELGGER IDIVLWDDNP AQFVINAMAP ADVASIVVDE
DKHTMDIAVE AGNLAQAIGR NGQNVRLASQ LSGWELNVMT VDDLQAKHQA EAHAAIDTFT
KYLDIDEDFA TVLVEEGFST LEELAYVPMK ELLEIEGLDE PTVEALRERA KNALATIAQA
QEESLGDNKP ADDLLNLEGV DRDLAFKLAA RGVCTLEDLA EQGIDDLADI EGLTDEKAGA
LIMAARNICW FGDEA
