NUSA_MYCGE
ID NUSA_MYCGE Reviewed; 531 AA.
AC P47387; Q49227;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN Name=nusA {ECO:0000255|HAMAP-Rule:MF_00945}; OrderedLocusNames=MG141;
OS Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS (Mycoplasmoides genitalium).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=243273;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-291.
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL J. Bacteriol. 175:7918-7930(1993).
CC -!- FUNCTION: Participates in both transcription termination and
CC antitermination. {ECO:0000255|HAMAP-Rule:MF_00945}.
CC -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC dependent RNA polymerase and to nascent RNA. {ECO:0000255|HAMAP-
CC Rule:MF_00945}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945}.
CC -!- SIMILARITY: Belongs to the NusA family. {ECO:0000255|HAMAP-
CC Rule:MF_00945}.
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DR EMBL; L43967; AAC71359.1; -; Genomic_DNA.
DR EMBL; U01778; AAD10598.1; -; Genomic_DNA.
DR PIR; F64215; F64215.
DR RefSeq; WP_009885827.1; NZ_AAGX01000007.1.
DR AlphaFoldDB; P47387; -.
DR SMR; P47387; -.
DR STRING; 243273.MG_141; -.
DR EnsemblBacteria; AAC71359; AAC71359; MG_141.
DR KEGG; mge:MG_141; -.
DR eggNOG; COG0195; Bacteria.
DR HOGENOM; CLU_029242_2_2_14; -.
DR OMA; RIPGERC; -.
DR OrthoDB; 384865at2; -.
DR BioCyc; MGEN243273:G1GJ2-163-MON; -.
DR Proteomes; UP000000807; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR GO; GO:0031564; P:transcription antitermination; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.1480.10; -; 1.
DR Gene3D; 3.30.300.20; -; 2.
DR HAMAP; MF_00945_B; NusA_B; 1.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR025249; KH_dom_NusA-like.
DR InterPro; IPR009019; KH_sf_prok-type.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR030842; NusA_bac.
DR InterPro; IPR036555; NusA_N_sf.
DR InterPro; IPR022967; S1_dom.
DR InterPro; IPR003029; S1_domain.
DR InterPro; IPR013735; TF_NusA_N.
DR InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR PANTHER; PTHR22648; PTHR22648; 1.
DR Pfam; PF13184; KH_5; 1.
DR Pfam; PF08529; NusA_N; 1.
DR SMART; SM00322; KH; 2.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF54814; SSF54814; 2.
DR SUPFAM; SSF69705; SSF69705; 1.
DR TIGRFAMs; TIGR01953; NusA; 1.
DR PROSITE; PS50084; KH_TYPE_1; 1.
DR PROSITE; PS50126; S1; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome; RNA-binding; Transcription;
KW Transcription antitermination; Transcription regulation;
KW Transcription termination.
FT CHAIN 1..531
FT /note="Transcription termination/antitermination protein
FT NusA"
FT /id="PRO_0000181971"
FT DOMAIN 165..235
FT /note="S1 motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT DOMAIN 340..410
FT /note="KH"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT REGION 463..531
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 463..479
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 487..501
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 278..280
FT /note="PAV -> ACSW (in Ref. 2; AAD10598)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 531 AA; 59751 MW; 28E61802F55CAF5D CRC64;
MKITFISGQE VSLGTSFLLF SKKIVMNELN QPLLAIIKNV AKTKNLSIEE VVFCLKTALE
QAYKKHLNFV NVEVNINFDK GIINVEQLFN VVSDENEDYD DFLEIPLQAA NKINSSLQLG
DVLRKPIPLK NISSDLINKM IAIFNQKISE TNFKAVMSEF SSEVGEVIEA KVEDIDTNKE
GGLKGYIINL ETTKGYISKR ELSKGERLEI GKKYLFVIKE IQRQASLWPI TLSRSDTRLL
QFLLTSNTPE IENGTIVIKK IERSPGVKSK IAVISNDPAV DPVAAILGPK GEKIRGISEE
FNGEIIDIVF WNEDKLKFLI NAILPAEVIG YNILQDDERD TSIEVVVPAN QIANVFGFKG
VNIRLISNLT GWNSVDVYSE KDASEANIKF TRLSFEPEGL FGIKKRREKI ISNDATDKVF
YTSKDNVIDD EIIVDLAKDL MVDNKQKQPE QVAKQVVEKS QLEKQVTPKE KEKVQPKAKV
HSNSHSKKPA KPNQIFSITV DASDKNLKKD QVDNNQTNPQ TKQTFDSFDD L
