ID   NUSA_MYCPN              Reviewed;         540 AA.
AC   P75591;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN   Name=nusA {ECO:0000255|HAMAP-Rule:MF_00945}; OrderedLocusNames=MPN_154;
GN   ORFNames=MP677;
OS   Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS   pneumoniae).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=11271496;
RX   DOI=10.1002/1522-2683(200011)21:17<3765::aid-elps3765>3.0.co;2-6;
RA   Regula J.T., Ueberle B., Boguth G., Goerg A., Schnoelzer M., Herrmann R.,
RA   Frank R.;
RT   "Towards a two-dimensional proteome map of Mycoplasma pneumoniae.";
RL   Electrophoresis 21:3765-3780(2000).
CC   -!- FUNCTION: Participates in both transcription termination and
CC       antitermination. {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC       dependent RNA polymerase and to nascent RNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- SIMILARITY: Belongs to the NusA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
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DR   EMBL; U00089; AAB96325.1; -; Genomic_DNA.
DR   PIR; S74003; S74003.
DR   RefSeq; NP_109842.1; NC_000912.1.
DR   RefSeq; WP_010874511.1; NC_000912.1.
DR   AlphaFoldDB; P75591; -.
DR   SMR; P75591; -.
DR   STRING; 272634.MPN_154; -.
DR   EnsemblBacteria; AAB96325; AAB96325; MPN_154.
DR   KEGG; mpn:MPN_154; -.
DR   PATRIC; fig|272634.6.peg.171; -.
DR   HOGENOM; CLU_029242_2_2_14; -.
DR   OMA; RIPGERC; -.
DR   BioCyc; MPNE272634:G1GJ3-259-MON; -.
DR   Proteomes; UP000000808; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR   GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.1480.10; -; 1.
DR   Gene3D; 3.30.300.20; -; 2.
DR   HAMAP; MF_00945_B; NusA_B; 1.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR015946; KH_dom-like_a/b.
DR   InterPro; IPR025249; KH_dom_NusA-like.
DR   InterPro; IPR009019; KH_sf_prok-type.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR030842; NusA_bac.
DR   InterPro; IPR036555; NusA_N_sf.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   InterPro; IPR013735; TF_NusA_N.
DR   InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR   PANTHER; PTHR22648; PTHR22648; 1.
DR   Pfam; PF13184; KH_5; 1.
DR   Pfam; PF08529; NusA_N; 1.
DR   SMART; SM00322; KH; 2.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF54814; SSF54814; 2.
DR   SUPFAM; SSF69705; SSF69705; 1.
DR   TIGRFAMs; TIGR01953; NusA; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
DR   PROSITE; PS50126; S1; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Reference proteome; RNA-binding; Transcription;
KW   Transcription antitermination; Transcription regulation;
KW   Transcription termination.
FT   CHAIN           1..540
FT                   /note="Transcription termination/antitermination protein
FT                   NusA"
FT                   /id="PRO_0000181972"
FT   DOMAIN          144..214
FT                   /note="S1 motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   DOMAIN          319..386
FT                   /note="KH"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   REGION          457..540
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        459..495
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   540 AA;  60272 MW;  0A30E0AE6B998A7F CRC64;
     MNNQSNHFTN PLLQLIKNVA ETKNLAIDDV VLCLKTALAQ TYKKHLNYVN VEVNIDFNKG
     LMQIEQLFDV VDDNNEDYDD FLEMPLSEAK KLNPNLEVGG VLRKPVSLKD IKGDLISKMV
     LLFNQKINET AFKTVMSDFI NEVGQVIEAR VEDIDTNKDG GLKGYIVNLE TTKGYMPKRE
     LSKGEKLDIG KKYLFVIKEI QKQSSMWPIT LSRSDSRLLE FLLNSNTPEI ANGTIEIKKM
     ERSPGTKSKV AVISKDPVVD PIAAILGPKG ERIRGISEEF NGEIIDIVIW NEDKLKFLVN
     AVLPAEVVGY NILQDDERDT SIEIVVPANQ IANVFGFKGI NIRLISNLTG WSSVDVYTEK
     DAAEQGIEFT RVNFQPQGIF GIKKRRDKIS NNPRNNNQQL ASDKVFYTSK ANVVDDEIIV
     DLAKQAEAKR VKQIKQEATK PELQLQQELN LEATPKVAAP TPTPAPQPTP APTKVEPVPP
     PVSVTPKPIP KVNKPKPVVK PKSVFSITVE ADDSKTKPEK SSAKTNTPQT KQTFDNFDDL