ID   NUSA_MYCTO              Reviewed;         347 AA.
AC   P9WIV2; L0TDQ4; O05818; P0A5M2;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN   Name=nusA {ECO:0000255|HAMAP-Rule:MF_00945}; OrderedLocusNames=MT2907;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Participates in both transcription termination and
CC       antitermination. {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC       dependent RNA polymerase and to nascent RNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- DOMAIN: Contains an N-terminal region that probably interacts with RNA
CC       polymerase and a C-terminal region composed of 3 RNA binding domains,
CC       S1, KH 1 and KH 2. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NusA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK47233.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK47233.1; ALT_INIT; Genomic_DNA.
DR   PIR; D70588; D70588.
DR   RefSeq; WP_003414511.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WIV2; -.
DR   SMR; P9WIV2; -.
DR   EnsemblBacteria; AAK47233; AAK47233; MT2907.
DR   GeneID; 45426828; -.
DR   KEGG; mtc:MT2907; -.
DR   PATRIC; fig|83331.31.peg.3141; -.
DR   HOGENOM; CLU_029242_2_2_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR   GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.1480.10; -; 1.
DR   Gene3D; 3.30.300.20; -; 2.
DR   HAMAP; MF_00945_B; NusA_B; 1.
DR   InterPro; IPR015946; KH_dom-like_a/b.
DR   InterPro; IPR025249; KH_dom_NusA-like.
DR   InterPro; IPR009019; KH_sf_prok-type.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR030842; NusA_bac.
DR   InterPro; IPR036555; NusA_N_sf.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   InterPro; IPR013735; TF_NusA_N.
DR   InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR   PANTHER; PTHR22648; PTHR22648; 1.
DR   Pfam; PF13184; KH_5; 1.
DR   Pfam; PF08529; NusA_N; 2.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF54814; SSF54814; 2.
DR   SUPFAM; SSF69705; SSF69705; 1.
DR   TIGRFAMs; TIGR01953; NusA; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
DR   PROSITE; PS50126; S1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Repeat; RNA-binding; Transcription;
KW   Transcription antitermination; Transcription regulation;
KW   Transcription termination.
FT   CHAIN           1..347
FT                   /note="Transcription termination/antitermination protein
FT                   NusA"
FT                   /id="PRO_0000427940"
FT   DOMAIN          112..184
FT                   /note="S1 motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   DOMAIN          220..286
FT                   /note="KH 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   DOMAIN          287..347
FT                   /note="KH 2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   REGION          322..347
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   347 AA;  37642 MW;  6939478599D5B763 CRC64;
     MNIDMAALHA IEVDRGISVN ELLETIKSAL LTAYRHTQGH QTDARIEIDR KTGVVRVIAR
     ETDEAGNLIS EWDDTPEGFG RIAATTARQV MLQRFRDAEN ERTYGEFSTR EGEIVAGVIQ
     RDSRANARGL VVVRIGTETK ASEGVIPAAE QVPGESYEHG NRLRCYVVGV TRGAREPLIT
     LSRTHPNLVR KLFSLEVPEI ADGSVEIVAV AREAGHRSKI AVRSNVAGLN AKGACIGPMG
     QRVRNVMSEL SGEKIDIIDY DDDPARFVAN ALSPAKVVSV SVIDQTARAA RVVVPDFQLS
     LAIGKEGQNA RLAARLTGWR IDIRGDAPPP PPGQPEPGVS RGMAHDR