NUSA_MYCTU
ID NUSA_MYCTU Reviewed; 347 AA.
AC P9WIV3; L0TDQ4; O05818; P0A5M2;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 47.
DE RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN Name=nusA {ECO:0000255|HAMAP-Rule:MF_00945}; OrderedLocusNames=Rv2841c;
GN ORFNames=MTCY16B7.01, MTCY24A1.16;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS), SUBUNIT, AND DOMAIN.
RX PubMed=11743725; DOI=10.1006/jmbi.2000.5144;
RA Gopal B., Haire L.F., Gamblin S.J., Dodson E.J., Lane A.N.,
RA Papavinasasundaram K.G., Colston M.J., Dodson G.;
RT "Crystal structure of the transcription elongation/anti-termination factor
RT NusA from Mycobacterium tuberculosis at 1.7 A resolution.";
RL J. Mol. Biol. 314:1087-1095(2001).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 105-347 IN COMPLEX WITH RNA,
RP INTERACTION WITH RNA, AND DOMAIN.
RX PubMed=16193062; DOI=10.1038/sj.emboj.7600829;
RA Beuth B., Pennell S., Arnvig K.B., Martin S.R., Taylor I.A.;
RT "Structure of a Mycobacterium tuberculosis NusA-RNA complex.";
RL EMBO J. 24:3576-3587(2005).
CC -!- FUNCTION: Participates in both transcription termination and
CC antitermination. {ECO:0000255|HAMAP-Rule:MF_00945}.
CC -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC dependent RNA polymerase and to nascent RNA. {ECO:0000255|HAMAP-
CC Rule:MF_00945, ECO:0000269|PubMed:11743725,
CC ECO:0000269|PubMed:16193062}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945}.
CC -!- DOMAIN: Contains an N-terminal region that probably interacts with RNA
CC polymerase and a C-terminal region composed of 3 RNA binding domains,
CC S1, KH 1 and KH 2. {ECO:0000269|PubMed:11743725,
CC ECO:0000269|PubMed:16193062}.
CC -!- SIMILARITY: Belongs to the NusA family. {ECO:0000255|HAMAP-
CC Rule:MF_00945}.
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DR EMBL; AL123456; CCP45642.1; -; Genomic_DNA.
DR PIR; D70588; D70588.
DR RefSeq; NP_217357.1; NC_000962.3.
DR RefSeq; WP_003414511.1; NZ_NVQJ01000006.1.
DR PDB; 1K0R; X-ray; 1.70 A; A/B=1-347.
DR PDB; 2ASB; X-ray; 1.50 A; A=105-347.
DR PDB; 2ATW; X-ray; 2.25 A; A/C=105-347.
DR PDBsum; 1K0R; -.
DR PDBsum; 2ASB; -.
DR PDBsum; 2ATW; -.
DR AlphaFoldDB; P9WIV3; -.
DR SMR; P9WIV3; -.
DR STRING; 83332.Rv2841c; -.
DR PaxDb; P9WIV3; -.
DR DNASU; 888213; -.
DR GeneID; 45426828; -.
DR GeneID; 888213; -.
DR KEGG; mtu:Rv2841c; -.
DR TubercuList; Rv2841c; -.
DR eggNOG; COG0195; Bacteria.
DR OMA; MDIDMSA; -.
DR PhylomeDB; P9WIV3; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IDA:MTBBASE.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR GO; GO:0031564; P:transcription antitermination; IDA:MTBBASE.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.1480.10; -; 1.
DR Gene3D; 3.30.300.20; -; 2.
DR HAMAP; MF_00945_B; NusA_B; 1.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR025249; KH_dom_NusA-like.
DR InterPro; IPR009019; KH_sf_prok-type.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR030842; NusA_bac.
DR InterPro; IPR036555; NusA_N_sf.
DR InterPro; IPR022967; S1_dom.
DR InterPro; IPR003029; S1_domain.
DR InterPro; IPR013735; TF_NusA_N.
DR InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR PANTHER; PTHR22648; PTHR22648; 1.
DR Pfam; PF13184; KH_5; 1.
DR Pfam; PF08529; NusA_N; 2.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF54814; SSF54814; 2.
DR SUPFAM; SSF69705; SSF69705; 1.
DR TIGRFAMs; TIGR01953; NusA; 1.
DR PROSITE; PS50084; KH_TYPE_1; 1.
DR PROSITE; PS50126; S1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome; Repeat; RNA-binding;
KW Transcription; Transcription antitermination; Transcription regulation;
KW Transcription termination.
FT CHAIN 1..347
FT /note="Transcription termination/antitermination protein
FT NusA"
FT /id="PRO_0000181974"
FT DOMAIN 112..184
FT /note="S1 motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT DOMAIN 220..286
FT /note="KH 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT DOMAIN 287..347
FT /note="KH 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT REGION 322..347
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 9..11
FT /evidence="ECO:0007829|PDB:1K0R"
FT STRAND 12..14
FT /evidence="ECO:0007829|PDB:1K0R"
FT TURN 21..24
FT /evidence="ECO:0007829|PDB:1K0R"
FT TURN 27..31
FT /evidence="ECO:0007829|PDB:1K0R"
FT HELIX 34..36
FT /evidence="ECO:0007829|PDB:1K0R"
FT STRAND 37..39
FT /evidence="ECO:0007829|PDB:1K0R"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:1K0R"
FT STRAND 64..67
FT /evidence="ECO:0007829|PDB:1K0R"
FT TURN 80..82
FT /evidence="ECO:0007829|PDB:1K0R"
FT TURN 84..88
FT /evidence="ECO:0007829|PDB:1K0R"
FT HELIX 89..96
FT /evidence="ECO:0007829|PDB:1K0R"
FT STRAND 114..120
FT /evidence="ECO:0007829|PDB:2ASB"
FT HELIX 123..127
FT /evidence="ECO:0007829|PDB:2ASB"
FT STRAND 131..135
FT /evidence="ECO:0007829|PDB:2ASB"
FT STRAND 137..140
FT /evidence="ECO:0007829|PDB:2ASB"
FT STRAND 142..146
FT /evidence="ECO:0007829|PDB:2ASB"
FT HELIX 148..150
FT /evidence="ECO:0007829|PDB:2ASB"
FT STRAND 162..171
FT /evidence="ECO:0007829|PDB:2ASB"
FT STRAND 174..176
FT /evidence="ECO:0007829|PDB:2ASB"
FT STRAND 178..184
FT /evidence="ECO:0007829|PDB:2ASB"
FT HELIX 186..196
FT /evidence="ECO:0007829|PDB:2ASB"
FT HELIX 198..201
FT /evidence="ECO:0007829|PDB:2ASB"
FT STRAND 204..213
FT /evidence="ECO:0007829|PDB:2ASB"
FT TURN 214..216
FT /evidence="ECO:0007829|PDB:2ASB"
FT STRAND 217..226
FT /evidence="ECO:0007829|PDB:2ASB"
FT HELIX 231..236
FT /evidence="ECO:0007829|PDB:2ASB"
FT HELIX 238..240
FT /evidence="ECO:0007829|PDB:2ASB"
FT HELIX 241..249
FT /evidence="ECO:0007829|PDB:2ASB"
FT TURN 250..252
FT /evidence="ECO:0007829|PDB:2ASB"
FT STRAND 254..259
FT /evidence="ECO:0007829|PDB:2ASB"
FT HELIX 264..271
FT /evidence="ECO:0007829|PDB:2ASB"
FT TURN 272..274
FT /evidence="ECO:0007829|PDB:2ASB"
FT STRAND 278..284
FT /evidence="ECO:0007829|PDB:2ASB"
FT TURN 285..288
FT /evidence="ECO:0007829|PDB:2ASB"
FT STRAND 289..294
FT /evidence="ECO:0007829|PDB:2ASB"
FT HELIX 296..298
FT /evidence="ECO:0007829|PDB:2ASB"
FT HELIX 299..303
FT /evidence="ECO:0007829|PDB:2ASB"
FT HELIX 305..307
FT /evidence="ECO:0007829|PDB:2ASB"
FT HELIX 308..317
FT /evidence="ECO:0007829|PDB:2ASB"
FT STRAND 320..327
FT /evidence="ECO:0007829|PDB:2ASB"
SQ SEQUENCE 347 AA; 37642 MW; 6939478599D5B763 CRC64;
MNIDMAALHA IEVDRGISVN ELLETIKSAL LTAYRHTQGH QTDARIEIDR KTGVVRVIAR
ETDEAGNLIS EWDDTPEGFG RIAATTARQV MLQRFRDAEN ERTYGEFSTR EGEIVAGVIQ
RDSRANARGL VVVRIGTETK ASEGVIPAAE QVPGESYEHG NRLRCYVVGV TRGAREPLIT
LSRTHPNLVR KLFSLEVPEI ADGSVEIVAV AREAGHRSKI AVRSNVAGLN AKGACIGPMG
QRVRNVMSEL SGEKIDIIDY DDDPARFVAN ALSPAKVVSV SVIDQTARAA RVVVPDFQLS
LAIGKEGQNA RLAARLTGWR IDIRGDAPPP PPGQPEPGVS RGMAHDR
