NUSA_SALTY
ID NUSA_SALTY Reviewed; 500 AA.
AC P37430;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN Name=nusA {ECO:0000255|HAMAP-Rule:MF_00945}; OrderedLocusNames=STM3287;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=8113180; DOI=10.1128/jb.176.5.1394-1404.1994;
RA Craven M.G., Granston A.E., Schauer A.T., Zheng C., Gray T.A.,
RA Friedman D.I.;
RT "Escherichia coli-Salmonella typhimurium hybrid nusA genes: identification
RT of a short motif required for action of the lambda N transcription
RT antitermination protein.";
RL J. Bacteriol. 176:1394-1404(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Participates in both transcription termination and
CC antitermination. {ECO:0000255|HAMAP-Rule:MF_00945}.
CC -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC dependent RNA polymerase and to nascent RNA. {ECO:0000255|HAMAP-
CC Rule:MF_00945}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945}.
CC -!- SIMILARITY: Belongs to the NusA family. {ECO:0000255|HAMAP-
CC Rule:MF_00945}.
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DR EMBL; M61008; AAA20049.1; -; Unassigned_RNA.
DR EMBL; AE006468; AAL22159.1; -; Genomic_DNA.
DR PIR; A53377; A53377.
DR RefSeq; NP_462200.1; NC_003197.2.
DR RefSeq; WP_001031038.1; NC_003197.2.
DR AlphaFoldDB; P37430; -.
DR SMR; P37430; -.
DR STRING; 99287.STM3287; -.
DR PaxDb; P37430; -.
DR EnsemblBacteria; AAL22159; AAL22159; STM3287.
DR GeneID; 1254810; -.
DR KEGG; stm:STM3287; -.
DR PATRIC; fig|99287.12.peg.3486; -.
DR HOGENOM; CLU_029242_0_0_6; -.
DR OMA; MDIDMSA; -.
DR PhylomeDB; P37430; -.
DR BioCyc; SENT99287:STM3287-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR GO; GO:0031564; P:transcription antitermination; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.1480.10; -; 1.
DR Gene3D; 3.30.300.20; -; 2.
DR HAMAP; MF_00945_B; NusA_B; 1.
DR InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR025249; KH_dom_NusA-like.
DR InterPro; IPR009019; KH_sf_prok-type.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR030842; NusA_bac.
DR InterPro; IPR036555; NusA_N_sf.
DR InterPro; IPR022967; S1_dom.
DR InterPro; IPR003029; S1_domain.
DR InterPro; IPR013735; TF_NusA_N.
DR InterPro; IPR010214; Tscrpt_termin_fac_NusA_C_rpt.
DR InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR PANTHER; PTHR22648; PTHR22648; 1.
DR Pfam; PF13184; KH_5; 1.
DR Pfam; PF08529; NusA_N; 1.
DR SMART; SM00322; KH; 2.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF47794; SSF47794; 2.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF54814; SSF54814; 2.
DR SUPFAM; SSF69705; SSF69705; 1.
DR TIGRFAMs; TIGR01953; NusA; 1.
DR TIGRFAMs; TIGR01954; nusA_Cterm_rpt; 2.
DR PROSITE; PS50084; KH_TYPE_1; 1.
DR PROSITE; PS50126; S1; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome; Repeat; RNA-binding; Transcription;
KW Transcription antitermination; Transcription regulation;
KW Transcription termination.
FT CHAIN 1..500
FT /note="Transcription termination/antitermination protein
FT NusA"
FT /id="PRO_0000181978"
FT DOMAIN 135..205
FT /note="S1 motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT DOMAIN 307..373
FT /note="KH"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT REPEAT 369..419
FT /note="1"
FT REPEAT 444..494
FT /note="2"
FT REGION 369..494
FT /note="2 X 51 AA approximate repeats"
FT CONFLICT 261
FT /note="G -> R (in Ref. 1; AAA20049)"
FT /evidence="ECO:0000305"
FT CONFLICT 445
FT /note="L -> V (in Ref. 1; AAA20049)"
FT /evidence="ECO:0000305"
FT CONFLICT 466
FT /note="D -> Q (in Ref. 1; AAA20049)"
FT /evidence="ECO:0000305"
FT CONFLICT 471
FT /note="D -> H (in Ref. 1; AAA20049)"
FT /evidence="ECO:0000305"
FT CONFLICT 485
FT /note="E -> A (in Ref. 1; AAA20049)"
FT /evidence="ECO:0000305"
FT CONFLICT 488
FT /note="M -> I (in Ref. 1; AAA20049)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 500 AA; 55426 MW; ABC07F5F16681B0F CRC64;
MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVEID RKSGDFDTFR
RWLIVEEVTM PTKEITLEAA RFEDESLNVG DYVEDQIESV TFDRITTQTA KQVIVQKVRE
AERAMVVDQF RDQEGEIVTG VVKKVNRDNI SLEIKSEGMA GNAEAVILRE DMLPRENFRP
GDRIRGVLYA VRPEARGAQL FVTRSKPEML IELFRIEVPE IGEEVIEIKA AARDPGSRAK
IAVKTNDKRI DPVGACVGMR GARVQAVSTE LGGERIDIVL WDDNPAQFVI NAMAPADVAS
IVVDEDKHTM DIAVEAGNLA QAIGRNGQNV RLASQLSGWE LNVMTVDDLQ AKHQAEAHAA
IEIFTKYLDI DEEFATVLVE EGFSTLEELA YVPMKELLEI DGLDEPTVEA LRERAKNALA
TLAQDQEASL GDNKPADDLL NLEGLDRDMA FKLAARGVCT LEDLADQGID DLADIEGLTD
EKAGELIMAA RNICWFGDEA
