ID   NUSA_THET8              Reviewed;         387 AA.
AC   P48514; Q5SKE2;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 140.
DE   RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN   Name=nusA {ECO:0000255|HAMAP-Rule:MF_00945}; OrderedLocusNames=TTHA0701;
OS   Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC   Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=300852;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9242984; DOI=10.1016/s0300-9084(97)83506-7;
RA   Vornlocher H.-P., Kreutzer R., Sprinzl M.;
RT   "Organization of the Thermus thermophilus nusA/infB operon and
RT   overexpression of the infB gene in Escherichia coli.";
RL   Biochimie 79:195-203(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27634 / DSM 579 / HB8;
RA   Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA   Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT   "Complete genome sequence of Thermus thermophilus HB8.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Participates in both transcription termination and
CC       antitermination. {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC       dependent RNA polymerase and to nascent RNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- SIMILARITY: Belongs to the NusA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
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DR   EMBL; Z48001; CAA88036.1; -; Genomic_DNA.
DR   EMBL; AP008226; BAD70524.1; -; Genomic_DNA.
DR   PIR; S52274; S52274.
DR   RefSeq; WP_011172799.1; NC_006461.1.
DR   RefSeq; YP_143967.1; NC_006461.1.
DR   AlphaFoldDB; P48514; -.
DR   SMR; P48514; -.
DR   STRING; 300852.55772083; -.
DR   EnsemblBacteria; BAD70524; BAD70524; BAD70524.
DR   GeneID; 3168103; -.
DR   KEGG; ttj:TTHA0701; -.
DR   PATRIC; fig|300852.9.peg.695; -.
DR   eggNOG; COG0195; Bacteria.
DR   HOGENOM; CLU_029242_2_2_0; -.
DR   OMA; MDIDMSA; -.
DR   PhylomeDB; P48514; -.
DR   Proteomes; UP000000532; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR   GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.1480.10; -; 1.
DR   Gene3D; 3.30.300.20; -; 2.
DR   HAMAP; MF_00945_B; NusA_B; 1.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR015946; KH_dom-like_a/b.
DR   InterPro; IPR025249; KH_dom_NusA-like.
DR   InterPro; IPR009019; KH_sf_prok-type.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR030842; NusA_bac.
DR   InterPro; IPR036555; NusA_N_sf.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   InterPro; IPR013735; TF_NusA_N.
DR   InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR   PANTHER; PTHR22648; PTHR22648; 1.
DR   Pfam; PF13184; KH_5; 1.
DR   Pfam; PF08529; NusA_N; 1.
DR   Pfam; PF00575; S1; 1.
DR   SMART; SM00322; KH; 2.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF54814; SSF54814; 2.
DR   SUPFAM; SSF69705; SSF69705; 1.
DR   TIGRFAMs; TIGR01953; NusA; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
DR   PROSITE; PS50126; S1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; RNA-binding; Transcription;
KW   Transcription antitermination; Transcription regulation;
KW   Transcription termination.
FT   CHAIN           1..387
FT                   /note="Transcription termination/antitermination protein
FT                   NusA"
FT                   /id="PRO_0000181980"
FT   DOMAIN          145..209
FT                   /note="S1 motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   DOMAIN          312..379
FT                   /note="KH"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
SQ   SEQUENCE   387 AA;  43939 MW;  3CDA7316BFE87FE9 CRC64;
     MNREFVEAMQ HLALERGVSV EEILEAFKEA LRKAYIKRQK GYKKEEIDQG KGPEVDVYID
     PNSGRIEMVE VRRVVEKVED PDKEIALAEA LQYDPEVQVG DEMEFPINPE ELSRMAIQDL
     RQILTQRLKE SERNRIYNEY KDKEGQVLTG VVTRVDNRGN VFVELGRGEA YLPRSEQIPT
     EKYHPGQRIK VYLKKVDKSA KGPSLLVSRA HEKLLEHLLK QEVPEIAEGI VEIKAIAREP
     GRRSKVAVTS HNPNVDPIGA CIGHKGQRIQ AVSAELGREK VDIILWSKDP KEFIRNALSP
     AQVGSIELDP EAKKARVKVT KDQHSLAIGT GGQNVRLASK LTGYDIHFEE AEISDLDEAL
     RRAAEEEAEA RERKAREEFE KLFRDLG