NUSB_AQUAE
ID NUSB_AQUAE Reviewed; 148 AA.
AC O66530;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Transcription antitermination protein NusB {ECO:0000255|HAMAP-Rule:MF_00073};
DE AltName: Full=Antitermination factor NusB {ECO:0000255|HAMAP-Rule:MF_00073};
GN Name=nusB {ECO:0000255|HAMAP-Rule:MF_00073}; OrderedLocusNames=aq_133;
OS Aquifex aeolicus (strain VF5).
OC Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX NCBI_TaxID=224324;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL Nature 392:353-358(1998).
CC -!- FUNCTION: Involved in transcription antitermination. Required for
CC transcription of ribosomal RNA (rRNA) genes. Binds specifically to the
CC boxA antiterminator sequence of the ribosomal RNA (rrn) operons.
CC {ECO:0000255|HAMAP-Rule:MF_00073}.
CC -!- SIMILARITY: Belongs to the NusB family. {ECO:0000255|HAMAP-
CC Rule:MF_00073, ECO:0000305}.
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DR EMBL; AE000657; AAC06491.1; -; Genomic_DNA.
DR PIR; G70312; G70312.
DR RefSeq; NP_213090.1; NC_000918.1.
DR RefSeq; WP_010880028.1; NC_000918.1.
DR PDB; 2JR0; NMR; -; A=1-148.
DR PDB; 3R2C; X-ray; 1.90 A; A/B=1-148.
DR PDB; 3R2D; X-ray; 2.20 A; A/B=1-148.
DR PDB; 4EYA; X-ray; 3.20 A; A/B/C/D/E/F/G/H=1-148.
DR PDBsum; 2JR0; -.
DR PDBsum; 3R2C; -.
DR PDBsum; 3R2D; -.
DR PDBsum; 4EYA; -.
DR AlphaFoldDB; O66530; -.
DR BMRB; O66530; -.
DR SMR; O66530; -.
DR STRING; 224324.aq_133; -.
DR EnsemblBacteria; AAC06491; AAC06491; aq_133.
DR KEGG; aae:aq_133; -.
DR eggNOG; COG0781; Bacteria.
DR HOGENOM; CLU_087843_3_0_0; -.
DR InParanoid; O66530; -.
DR OMA; DAFLILY; -.
DR OrthoDB; 1574817at2; -.
DR EvolutionaryTrace; O66530; -.
DR Proteomes; UP000000798; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-KW.
DR Gene3D; 1.10.940.10; -; 1.
DR HAMAP; MF_00073; NusB; 1.
DR InterPro; IPR035926; NusB-like_sf.
DR InterPro; IPR011605; NusB_fam.
DR InterPro; IPR006027; NusB_RsmB_TIM44.
DR PANTHER; PTHR11078; PTHR11078; 1.
DR Pfam; PF01029; NusB; 1.
DR SUPFAM; SSF48013; SSF48013; 1.
DR TIGRFAMs; TIGR01951; nusB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; RNA-binding; Transcription;
KW Transcription antitermination; Transcription regulation.
FT CHAIN 1..148
FT /note="Transcription antitermination protein NusB"
FT /id="PRO_0000176504"
FT HELIX 3..21
FT /evidence="ECO:0007829|PDB:3R2C"
FT HELIX 25..36
FT /evidence="ECO:0007829|PDB:3R2C"
FT HELIX 41..56
FT /evidence="ECO:0007829|PDB:3R2C"
FT HELIX 58..66
FT /evidence="ECO:0007829|PDB:3R2C"
FT TURN 67..71
FT /evidence="ECO:0007829|PDB:3R2C"
FT HELIX 74..76
FT /evidence="ECO:0007829|PDB:3R2C"
FT HELIX 79..92
FT /evidence="ECO:0007829|PDB:3R2C"
FT TURN 93..95
FT /evidence="ECO:0007829|PDB:2JR0"
FT HELIX 100..114
FT /evidence="ECO:0007829|PDB:3R2C"
FT HELIX 117..137
FT /evidence="ECO:0007829|PDB:3R2C"
SQ SEQUENCE 148 AA; 17112 MW; 6297579CA996E22F CRC64;
MRYRKGARDT AFLVLYRWDL RGENPGELFK EVVEEKNIKN KDAYEYAKKL VDTAVRHIEE
IDSIIEKHLK GWSIDRLGYV ERNALRLGVA ELIFLKSKEP GRVFIDIVDL VKKYADEKAG
KFVNGVLSAI YKAYITSSKE EKPSLKSE
