ARP8_ARATH
ID ARP8_ARATH Reviewed; 471 AA.
AC Q9FKT0; Q7PCC3; Q84WA6; Q8L8Z7; Q8LKQ9;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Actin-related protein 8;
DE AltName: Full=F-box protein ARP8;
GN Name=ARP8; OrderedLocusNames=At5g56180; ORFNames=MDA7.26;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 4), IDENTIFICATION, TISSUE
RP SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=11891255; DOI=10.1104/pp.010906;
RA McKinney E.C., Kandasamy M.K., Meagher R.B.;
RT "Arabidopsis contains ancient classes of differentially expressed actin-
RT related protein genes.";
RL Plant Physiol. 128:997-1007(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP REVIEW, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15063870; DOI=10.1016/j.tplants.2004.02.004;
RA Kandasamy M.K., Deal R.B., McKinney E.C., Meagher R.B.;
RT "Plant actin-related proteins.";
RL Trends Plant Sci. 9:196-202(2004).
RN [7]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=18385164; DOI=10.1093/pcp/pcn053;
RA Kandasamy M.K., McKinney E.C., Meagher R.B.;
RT "ACTIN-RELATED PROTEIN8 encodes an F-box protein localized to the nucleolus
RT in Arabidopsis.";
RL Plant Cell Physiol. 49:858-863(2008).
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:18385164}.
CC Cytoplasm {ECO:0000269|PubMed:18385164}. Note=Localized to the
CC nucleolus in interphase cells and dispersed in the cytoplasm in mitotic
CC cells.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1; Synonyms=ARP8B;
CC IsoId=Q9FKT0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9FKT0-2; Sequence=VSP_022941, VSP_022942;
CC Name=3;
CC IsoId=Q9FKT0-3; Sequence=VSP_022939;
CC Name=4; Synonyms=ARP8A;
CC IsoId=Q9FKT0-4; Sequence=VSP_022940;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed in all organs and cell
CC types. Higher expression in seedlings. {ECO:0000269|PubMed:11891255,
CC ECO:0000269|PubMed:18385164}.
CC -!- SIMILARITY: Belongs to the actin family. Plant ARP8 subfamily.
CC {ECO:0000305}.
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DR EMBL; AF507916; AAM53248.1; -; mRNA.
DR EMBL; AF507917; AAM53249.1; -; mRNA.
DR EMBL; AB011476; BAB09300.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96730.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96731.1; -; Genomic_DNA.
DR EMBL; BT004068; AAO42095.1; -; mRNA.
DR EMBL; AY088717; AAM67035.1; -; mRNA.
DR EMBL; BK000426; DAA00031.1; -; Genomic_DNA.
DR EMBL; BK000426; DAA00032.1; -; Genomic_DNA.
DR RefSeq; NP_568836.2; NM_125000.4. [Q9FKT0-1]
DR RefSeq; NP_974940.1; NM_203211.1. [Q9FKT0-2]
DR AlphaFoldDB; Q9FKT0; -.
DR SMR; Q9FKT0; -.
DR STRING; 3702.AT5G56180.1; -.
DR PaxDb; Q9FKT0; -.
DR PRIDE; Q9FKT0; -.
DR ProteomicsDB; 246891; -. [Q9FKT0-1]
DR EnsemblPlants; AT5G56180.1; AT5G56180.1; AT5G56180. [Q9FKT0-1]
DR EnsemblPlants; AT5G56180.2; AT5G56180.2; AT5G56180. [Q9FKT0-2]
DR GeneID; 835717; -.
DR Gramene; AT5G56180.1; AT5G56180.1; AT5G56180. [Q9FKT0-1]
DR Gramene; AT5G56180.2; AT5G56180.2; AT5G56180. [Q9FKT0-2]
DR KEGG; ath:AT5G56180; -.
DR Araport; AT5G56180; -.
DR TAIR; locus:2161795; AT5G56180.
DR eggNOG; KOG0676; Eukaryota.
DR HOGENOM; CLU_036757_0_0_1; -.
DR InParanoid; Q9FKT0; -.
DR OrthoDB; 572248at2759; -.
DR PhylomeDB; Q9FKT0; -.
DR PRO; PR:Q9FKT0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FKT0; baseline and differential.
DR Genevisible; Q9FKT0; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005869; C:dynactin complex; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005200; F:structural constituent of cytoskeleton; ISS:TAIR.
DR GO; GO:0030029; P:actin filament-based process; TAS:TAIR.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR InterPro; IPR004000; Actin.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR PANTHER; PTHR11937; PTHR11937; 1.
DR Pfam; PF00022; Actin; 1.
DR Pfam; PF12937; F-box-like; 1.
DR SMART; SM00268; ACTIN; 1.
DR SMART; SM00256; FBOX; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Cytoplasm; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..471
FT /note="Actin-related protein 8"
FT /id="PRO_0000274923"
FT DOMAIN 40..86
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT BINDING 256..259
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT VAR_SEQ 164..165
FT /note="EF -> VR (in isoform 3)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_022939"
FT VAR_SEQ 233..462
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:11891255"
FT /id="VSP_022940"
FT VAR_SEQ 364..387
FT /note="MRAMSLHQAVSLCMDHCDAAGLTG -> IIVHKLVYEFHGFPYAYQGWAIFL
FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_022941"
FT VAR_SEQ 388..471
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_022942"
FT CONFLICT 24
FT /note="Q -> H (in Ref. 1; AAM53249)"
FT /evidence="ECO:0000305"
FT CONFLICT 260
FT /note="Q -> K (in Ref. 4; AAO42095)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 471 AA; 52510 MW; 6BA4ABAE3AB8DE6E CRC64;
MILKKVWGSV WNRSNSGKDL VNHQRAIDVP PLLLSSSSSL GAFDQLPMDI LVQILMMMEP
KDAVKLGLTC KAWKCVASGN RLWIFYLQCS QEPWDSIFFA ETSLRSGYPL RMISSQSGEL
SFMHIYSQRA QVPGSIIIDG GSGYCKFGWS KYASPSGRSA TFLEFGNIES PIYARLQQFF
ATIFTRMQVK PSMQPIVVSL PLCHFDDTES AKASRRQLKT AIFNVLFDMN VPAVCAVNQA
VLALYAARRT SGIVVNIGFQ VITILPILHG KVMRQVGVEV IGFGALKLTG FLKEKMQENN
ISFQSLYTVR TLKEKLCYVA LDYKAELSKD TQASVEVSGE GWFTLSKERF QTGEILFQPR
LAGMRAMSLH QAVSLCMDHC DAAGLTGDDS WFKTVVLTGG SACLPGLSER LERELQDHLP
SSISNGIRVI PPPYGVDTSW HGAKLISNLS IFPGPWCITR KQFRRKSRLM W