NUSB_MYCTU
ID NUSB_MYCTU Reviewed; 156 AA.
AC P9WIV1; L0TCN2; P95020;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 38.
DE RecName: Full=Transcription antitermination protein NusB {ECO:0000255|HAMAP-Rule:MF_00073};
DE AltName: Full=Antitermination factor NusB {ECO:0000255|HAMAP-Rule:MF_00073};
GN Name=nusB {ECO:0000255|HAMAP-Rule:MF_00073, ECO:0000303|PubMed:10881194};
GN OrderedLocusNames=Rv2533c; ORFNames=MTCY159.23;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA Raman K., Yeturu K., Chandra N.;
RT "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT through an interactome, reactome and genome-scale structural analysis.";
RL BMC Syst. Biol. 2:109-109(2008).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), AND SUBUNIT.
RX PubMed=10881194; DOI=10.1038/75876;
RA Gopal B., Haire L.F., Cox R.A., Colston M.J., Major S., Brannigan J.A.,
RA Smerdon S.J., Dodson G.;
RT "The crystal structure of NusB from Mycobacterium tuberculosis.";
RL Nat. Struct. Biol. 7:475-478(2000).
CC -!- FUNCTION: Involved in transcription antitermination. Required for
CC transcription of ribosomal RNA (rRNA) genes. Binds specifically to the
CC boxA antiterminator sequence of the ribosomal RNA (rrn) operons.
CC {ECO:0000255|HAMAP-Rule:MF_00073}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:10881194}.
CC -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC {ECO:0000269|PubMed:19099550}.
CC -!- SIMILARITY: Belongs to the NusB family. {ECO:0000255|HAMAP-
CC Rule:MF_00073, ECO:0000305}.
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DR EMBL; AL123456; CCP45328.1; -; Genomic_DNA.
DR PIR; A70658; A70658.
DR RefSeq; NP_217049.1; NC_000962.3.
DR RefSeq; WP_003899364.1; NZ_NVQJ01000032.1.
DR PDB; 1EYV; X-ray; 1.60 A; A/B=1-156.
DR PDBsum; 1EYV; -.
DR AlphaFoldDB; P9WIV1; -.
DR SMR; P9WIV1; -.
DR STRING; 83332.Rv2533c; -.
DR PaxDb; P9WIV1; -.
DR GeneID; 887359; -.
DR KEGG; mtu:Rv2533c; -.
DR TubercuList; Rv2533c; -.
DR eggNOG; COG0781; Bacteria.
DR OMA; DRMPVVD; -.
DR PhylomeDB; P9WIV1; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-KW.
DR Gene3D; 1.10.940.10; -; 1.
DR HAMAP; MF_00073; NusB; 1.
DR InterPro; IPR035926; NusB-like_sf.
DR InterPro; IPR011605; NusB_fam.
DR InterPro; IPR006027; NusB_RsmB_TIM44.
DR PANTHER; PTHR11078; PTHR11078; 1.
DR Pfam; PF01029; NusB; 1.
DR SUPFAM; SSF48013; SSF48013; 1.
DR TIGRFAMs; TIGR01951; nusB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; RNA-binding; Transcription;
KW Transcription antitermination; Transcription regulation.
FT CHAIN 1..156
FT /note="Transcription antitermination protein NusB"
FT /id="PRO_0000176557"
FT HELIX 10..27
FT /evidence="ECO:0007829|PDB:1EYV"
FT HELIX 31..44
FT /evidence="ECO:0007829|PDB:1EYV"
FT HELIX 53..64
FT /evidence="ECO:0007829|PDB:1EYV"
FT HELIX 66..74
FT /evidence="ECO:0007829|PDB:1EYV"
FT HELIX 82..84
FT /evidence="ECO:0007829|PDB:1EYV"
FT HELIX 87..102
FT /evidence="ECO:0007829|PDB:1EYV"
FT HELIX 108..122
FT /evidence="ECO:0007829|PDB:1EYV"
FT HELIX 127..138
FT /evidence="ECO:0007829|PDB:1EYV"
SQ SEQUENCE 156 AA; 16740 MW; 84923E642CA5FD15 CRC64;
MSDRKPVRGR HQARKRAVAL LFEAEVRGIS AAEVVDTRAA LAEAKPDIAR LHPYTAAVAR
GVSEHAAHID DLITAHLRGW TLDRLPAVDR AILRVSVWEL LHAADVPEPV VVDEAVQLAK
ELSTDDSPGF VNGVLGQVML VTPQLRAAAQ AVRGGA
