ID   ARPB_PSEPU              Reviewed;        1050 AA.
AC   Q9KJC2;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Antibiotic efflux pump membrane transporter ArpB;
GN   Name=arpB;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], EFFLUX PUMP SUBSTRATES, AND INDUCTION.
RC   STRAIN=ATCC 700801 / S12;
RX   PubMed=11160799; DOI=10.1099/00221287-147-1-43;
RA   Kieboom J., de Bont J.A.M.;
RT   "Identification and molecular characterization of an efflux system involved
RT   in Pseudomonas putida S12 multidrug resistance.";
RL   Microbiology 147:43-51(2001).
CC   -!- FUNCTION: The inner membrane transporter component of an antibiotic
CC       efflux pump. Confers resistance to numerous structurally unrelated
CC       antibiotics such as carbenicillin, chloramphenicol, erythromycin,
CC       novobiocin, streptomycin and tetracycline. Is not involved in organic
CC       solvent efflux.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- INDUCTION: The arpABC operon was not seen to be induced by
CC       carbenicillin, chloramphenicol, erythromycin nor by hexane, toluene or
CC       p-xylene. {ECO:0000269|PubMed:11160799}.
CC   -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC       (TC 2.A.6) family. {ECO:0000305}.
CC   -!- CAUTION: Despite being nearly identical to the ttgABC operon in strain
CC       DOT-T1E and the mepABC operon in strain KT2442-TOL this operon does not
CC       function in solvent efflux. This may be due to different protein
CC       expression levels. In strain KT2440 the equivalent operon does not seem
CC       to function in toluene efflux. {ECO:0000305}.
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DR   EMBL; AF183959; AAF73832.1; -; Genomic_DNA.
DR   RefSeq; WP_014592250.1; NZ_PZKR01000020.1.
DR   AlphaFoldDB; Q9KJC2; -.
DR   SMR; Q9KJC2; -.
DR   STRING; 1240350.AMZE01000001_gene2850; -.
DR   TCDB; 2.A.6.2.8; the resistance-nodulation-cell division (rnd) superfamily.
DR   eggNOG; COG0841; Bacteria.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0042908; P:xenobiotic transport; IEA:InterPro.
DR   Gene3D; 3.30.2090.10; -; 2.
DR   InterPro; IPR027463; AcrB_DN_DC_subdom.
DR   InterPro; IPR001036; Acrflvin-R.
DR   InterPro; IPR004764; HAE1.
DR   PANTHER; PTHR32063; PTHR32063; 1.
DR   Pfam; PF00873; ACR_tran; 1.
DR   PRINTS; PR00702; ACRIFLAVINRP.
DR   SUPFAM; SSF82714; SSF82714; 2.
DR   TIGRFAMs; TIGR00915; 2A0602; 1.
PE   2: Evidence at transcript level;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1050
FT                   /note="Antibiotic efflux pump membrane transporter ArpB"
FT                   /id="PRO_0000161850"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        339..359
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        370..390
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        393..413
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        440..460
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        472..492
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        539..559
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        871..891
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        893..913
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        923..943
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        972..992
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1004..1024
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1050 AA;  112794 MW;  CEC4543DC0C5E49C CRC64;
     MSKFFIDRPI FAWVIALVIM LVGALSILKL PINQYPSIAP PAIAIAVTYP GASAQTVQDT
     VVQVIEQQLN GIDNLRYVSS ESNSDGSMTI TATFEQGTNP DTAQVQVQNK LNLATPLLPQ
     EVQQQGIRVT KAVKNFLLVI GLVSEDGSMT KDDLANYIVS NMQDPISRTA GVGDFQVFGA
     QYAMRIWLDP AKLNKFQLTP VDVKTAVAAQ NVQVSSGQLG GLPALPGTQL NATIIGKTRL
     QTAEQFESIL LKVNKDGSQV RLGDVAQVGL GGENYAVSAQ FNGKPASGLA VKLATGANAL
     DTAKALRETI KGLEPFFPPG VKAVFPYDTT PVVTESISGV IHTLIEAVVL VFLVMYLFLQ
     NFRATIITTM TVPVVLLGTF GILAAAGFSI NTLTMFAMVL AIGLLVDDAI VVVENVERVM
     SEEGLPPKEA TKRSMEQIQG ALVGIALVLS AVLLPMAFFG GSTGVIYRQF SITIVSAMGL
     SVLVALIFTP ALCATMLKPL KKGEHHTAKG GFFGWFNRNF DRSVNGYERS VGTILRNKVP
     FLLGYALIVV GMIWLFARIP TAFLPEEDQG VLFAQVQTPA GSSAERTQVV VDQMREYLLK
     DEADTVSSVF TVNGFNFAGR GQSSGMAFIM LKPWDERSKE NSVFALAQRA QQHFFTFRDA
     MVFAFAPPAV LELGNATGFD VFLQDRGGVG HAKLMEARNQ FLAKAAQSKI LSAVRPNGLN
     DEPQYQLTID DERASALGVT IADINNTLSI ALGASYVNDF IDRGRVKKVY IQGEPSARMS
     PEDLQKWYVR NGAGEMVPFS SFAKGEWTYG SPKLSRYNGV EAMEILGAPA PGYSTGEAMA
     EVERIAGELP SGIGFSWTGM SYEEKLSGSQ MPALFALSVL FVFLCLAALY ESWSIPIAVV
     LVVPLGIIGA LIATSLRGLS NDVYFLVGLL TTIGLAAKNA ILIVEFAKEL HEQGRSLYDA
     AIEACRMRLR PIIMTSLAFI LGVVPLTIAS GAGAGSQHAI GTGVIGGMIS ATVLAIFWVP
     LFFVAVSSLF GSKEPEKDVT PENPRYEAGQ