ID   NUSG_AQUAE              Reviewed;         248 AA.
AC   O67757;
DT   20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 136.
DE   RecName: Full=Transcription termination/antitermination protein NusG {ECO:0000255|HAMAP-Rule:MF_00948};
GN   Name=nusG {ECO:0000255|HAMAP-Rule:MF_00948}; OrderedLocusNames=aq_1931;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT, AND DOMAIN.
RX   PubMed=12198166; DOI=10.1093/emboj/cdf455;
RA   Steiner T., Kaiser J.T., Marinkovic S., Huber R., Wahl M.C.;
RT   "Crystal structures of transcription factor NusG in light of its nucleic
RT   acid- and protein-binding activities.";
RL   EMBO J. 21:4641-4653(2002).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS), AND SUBUNIT.
RX   PubMed=12600194; DOI=10.1021/bi0272508;
RA   Knowlton J.R., Bubunenko M., Andrykovitch M., Guo W., Routzahn K.M.,
RA   Waugh D.S., Court D.L., Ji X.;
RT   "A spring-loaded state of NusG in its functional cycle is suggested by X-
RT   ray crystallography and supported by site-directed mutants.";
RL   Biochemistry 42:2275-2281(2003).
CC   -!- FUNCTION: Participates in transcription elongation, termination and
CC       antitermination. {ECO:0000255|HAMAP-Rule:MF_00948}.
CC   -!- SUBUNIT: Monomer (PubMed:12198166). Homodimer (PubMed:12600194).
CC       {ECO:0000269|PubMed:12198166, ECO:0000269|PubMed:12600194}.
CC   -!- DOMAIN: Contains an N-terminal RNP-like domain, a C-terminal element
CC       with a KOW sequence motif and a species-specific immunoglobulin-like
CC       fold. {ECO:0000269|PubMed:12198166}.
CC   -!- SIMILARITY: Belongs to the NusG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00948}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000657; AAC07722.1; -; Genomic_DNA.
DR   PIR; A70466; A70466.
DR   RefSeq; NP_214326.1; NC_000918.1.
DR   RefSeq; WP_010881262.1; NC_000918.1.
DR   PDB; 1M1G; X-ray; 2.00 A; A/B/C/D=1-248.
DR   PDB; 1M1H; X-ray; 1.95 A; A=1-248.
DR   PDB; 1NPP; X-ray; 2.00 A; A/B/C/D=1-248.
DR   PDB; 1NPR; X-ray; 2.21 A; A=1-248.
DR   PDBsum; 1M1G; -.
DR   PDBsum; 1M1H; -.
DR   PDBsum; 1NPP; -.
DR   PDBsum; 1NPR; -.
DR   AlphaFoldDB; O67757; -.
DR   SMR; O67757; -.
DR   STRING; 224324.aq_1931; -.
DR   EnsemblBacteria; AAC07722; AAC07722; aq_1931.
DR   KEGG; aae:aq_1931; -.
DR   PATRIC; fig|224324.8.peg.1496; -.
DR   eggNOG; COG0250; Bacteria.
DR   HOGENOM; CLU_067287_1_0_0; -.
DR   InParanoid; O67757; -.
DR   OMA; IEGPFMN; -.
DR   OrthoDB; 2027986at2; -.
DR   EvolutionaryTrace; O67757; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0006354; P:DNA-templated transcription, elongation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR   GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR   GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.30.30.30; -; 1.
DR   Gene3D; 2.60.320.10; -; 1.
DR   Gene3D; 3.30.70.940; -; 1.
DR   HAMAP; MF_00948; NusG; 1.
DR   InterPro; IPR005824; KOW.
DR   InterPro; IPR006645; NGN_dom.
DR   InterPro; IPR036735; NGN_dom_sf.
DR   InterPro; IPR043425; NusG-like.
DR   InterPro; IPR038690; NusG_2_sf.
DR   InterPro; IPR024045; NusG_dom2.
DR   InterPro; IPR014722; Rib_L2_dom2.
DR   InterPro; IPR001062; Transcrpt_antiterm_NusG.
DR   InterPro; IPR015869; Transcrpt_antiterm_NusG_bac_CS.
DR   InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR   PANTHER; PTHR30265; PTHR30265; 1.
DR   PANTHER; PTHR30265:SF2; PTHR30265:SF2; 1.
DR   Pfam; PF00467; KOW; 1.
DR   Pfam; PF02357; NusG; 1.
DR   Pfam; PF07009; NusG_II; 1.
DR   PRINTS; PR00338; NUSGTNSCPFCT.
DR   SMART; SM00739; KOW; 1.
DR   SMART; SM00738; NGN; 1.
DR   SUPFAM; SSF50104; SSF50104; 1.
DR   SUPFAM; SSF82679; SSF82679; 1.
DR   TIGRFAMs; TIGR00922; nusG; 1.
DR   PROSITE; PS01014; NUSG; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome; Transcription;
KW   Transcription antitermination; Transcription regulation;
KW   Transcription termination.
FT   CHAIN           1..248
FT                   /note="Transcription termination/antitermination protein
FT                   NusG"
FT                   /id="PRO_0000113914"
FT   DOMAIN          197..227
FT                   /note="KOW"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00948"
FT   STRAND          12..18
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   HELIX           23..36
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   HELIX           40..42
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          43..47
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          49..58
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          61..71
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          73..78
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          80..88
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          93..97
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   HELIX           103..106
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          116..119
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   TURN            120..123
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          124..131
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          133..135
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          138..143
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   HELIX           147..154
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          159..162
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          164..169
FT                   /evidence="ECO:0007829|PDB:1NPR"
FT   HELIX           174..183
FT                   /evidence="ECO:0007829|PDB:1M1H"
FT   STRAND          200..203
FT                   /evidence="ECO:0007829|PDB:1M1G"
FT   TURN            207..210
FT                   /evidence="ECO:0007829|PDB:1M1G"
FT   STRAND          212..219
FT                   /evidence="ECO:0007829|PDB:1M1G"
FT   TURN            220..223
FT                   /evidence="ECO:0007829|PDB:1M1G"
FT   STRAND          224..231
FT                   /evidence="ECO:0007829|PDB:1M1G"
FT   STRAND          234..241
FT                   /evidence="ECO:0007829|PDB:1M1G"
FT   STRAND          244..248
FT                   /evidence="ECO:0007829|PDB:1M1G"
SQ   SEQUENCE   248 AA;  27999 MW;  CECE083CA19D8482 CRC64;
     MSEQQVQELE KKWYALQVEP GKENEAKENL LKVLELEGLK DLVDEVIVPA EEKVVIRAQG
     KEKYRLSLKG NARDISVLGK KGVTTFRIEN GEVKVVESVE GDTCVNAPPI SKPGQKITCK
     ENKTEAKIVL DNKIFPGYIL IKAHMNDKLL MAIEKTPHVF RPVMVGGKPV PLKEEEVQNI
     LNQIKRGVKP SKVEFEKGDQ VRVIEGPFMN FTGTVEEVHP EKRKLTVMIS IFGRMTPVEL
     DFDQVEKI