ID   NUSG_BACSU              Reviewed;         177 AA.
AC   Q06795;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Transcription termination/antitermination protein NusG {ECO:0000255|HAMAP-Rule:MF_00948};
GN   Name=nusG {ECO:0000255|HAMAP-Rule:MF_00948}; OrderedLocusNames=BSU01010;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7968510; DOI=10.1111/j.1365-2958.1993.tb00910.x;
RA   Jeong S., Yoshikawa H., Takahashi H.;
RT   "Isolation and characterization of the secE homologue gene of Bacillus
RT   subtilis.";
RL   Mol. Microbiol. 10:133-142(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION IN RNA POLYMERASE PAUSING.
RX   PubMed=18852477; DOI=10.1073/pnas.0808842105;
RA   Yakhnin A.V., Yakhnin H., Babitzke P.;
RT   "Function of the Bacillus subtilis transcription elongation factor NusG in
RT   hairpin-dependent RNA polymerase pausing in the trp leader.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:16131-16136(2008).
RN   [4]
RP   FUNCTION.
RX   PubMed=20384694; DOI=10.1111/j.1365-2958.2010.07126.x;
RA   Yakhnin A.V., Babitzke P.;
RT   "Mechanism of NusG-stimulated pausing, hairpin-dependent pause site
RT   selection and intrinsic termination at overlapping pause and termination
RT   sites in the Bacillus subtilis trp leader.";
RL   Mol. Microbiol. 76:690-705(2010).
CC   -!- FUNCTION: Participates in transcription elongation, termination and
CC       antitermination. Stimulates RNA polymerase pausing at U107 and U144 in
CC       the trp leader. NusG-stimulated pausing is sequence specific. Does not
CC       affect trp leader termination. {ECO:0000255|HAMAP-Rule:MF_00948,
CC       ECO:0000269|PubMed:18852477, ECO:0000269|PubMed:20384694}.
CC   -!- SIMILARITY: Belongs to the NusG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00948}.
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DR   EMBL; D13303; BAA02560.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11877.1; -; Genomic_DNA.
DR   PIR; S39859; S39859.
DR   RefSeq; NP_387982.1; NC_000964.3.
DR   RefSeq; WP_003225764.1; NZ_JNCM01000029.1.
DR   AlphaFoldDB; Q06795; -.
DR   SMR; Q06795; -.
DR   STRING; 224308.BSU01010; -.
DR   PaxDb; Q06795; -.
DR   PRIDE; Q06795; -.
DR   EnsemblBacteria; CAB11877; CAB11877; BSU_01010.
DR   GeneID; 64301939; -.
DR   GeneID; 936851; -.
DR   KEGG; bsu:BSU01010; -.
DR   PATRIC; fig|224308.179.peg.104; -.
DR   eggNOG; COG0250; Bacteria.
DR   InParanoid; Q06795; -.
DR   OMA; DGPFNGF; -.
DR   PhylomeDB; Q06795; -.
DR   BioCyc; BSUB:BSU01010-MON; -.
DR   PRO; PR:Q06795; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0006354; P:DNA-templated transcription, elongation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR   GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR   GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.30.30.30; -; 1.
DR   Gene3D; 3.30.70.940; -; 1.
DR   HAMAP; MF_00948; NusG; 1.
DR   InterPro; IPR005824; KOW.
DR   InterPro; IPR006645; NGN_dom.
DR   InterPro; IPR036735; NGN_dom_sf.
DR   InterPro; IPR043425; NusG-like.
DR   InterPro; IPR014722; Rib_L2_dom2.
DR   InterPro; IPR001062; Transcrpt_antiterm_NusG.
DR   InterPro; IPR015869; Transcrpt_antiterm_NusG_bac_CS.
DR   InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR   PANTHER; PTHR30265; PTHR30265; 1.
DR   PANTHER; PTHR30265:SF2; PTHR30265:SF2; 1.
DR   Pfam; PF00467; KOW; 1.
DR   Pfam; PF02357; NusG; 1.
DR   PRINTS; PR00338; NUSGTNSCPFCT.
DR   SMART; SM00739; KOW; 1.
DR   SMART; SM00738; NGN; 1.
DR   SUPFAM; SSF50104; SSF50104; 1.
DR   SUPFAM; SSF82679; SSF82679; 1.
DR   TIGRFAMs; TIGR00922; nusG; 1.
DR   PROSITE; PS01014; NUSG; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Transcription; Transcription antitermination;
KW   Transcription regulation; Transcription termination.
FT   CHAIN           1..177
FT                   /note="Transcription termination/antitermination protein
FT                   NusG"
FT                   /id="PRO_0000113916"
FT   DOMAIN          128..156
FT                   /note="KOW"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00948"
SQ   SEQUENCE   177 AA;  20126 MW;  C9F2557AC35262EF CRC64;
     MEKNWYVVHT YSGYENKVKA NLEKRVESMG MQDKIFRVVV PEEEETDIKN GKKKVVKKKV
     FPGYVLVEIV MTDDSWYVVR NTPGVTGFVG SAGSGSKPTP LLPGEAETIL KRMGMDERKT
     DIDFELKETV KVIDGPFANF TGSIEEIDYD KSKVKVFVNM FGRETPVELE FTQIDKL