NUSG_MYCTU
ID NUSG_MYCTU Reviewed; 238 AA.
AC P9WIU9; L0T7B3; P65589; P96930;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 38.
DE RecName: Full=Transcription termination/antitermination protein NusG {ECO:0000255|HAMAP-Rule:MF_00948};
GN Name=nusG {ECO:0000255|HAMAP-Rule:MF_00948}; OrderedLocusNames=Rv0639;
GN ORFNames=MTCY20H10.20;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP LACK OF INTERACTION WITH RHO.
RX PubMed=20026069; DOI=10.1016/j.jmb.2009.12.022;
RA Kalarickal N.C., Ranjan A., Kalyani B.S., Wal M., Sen R.;
RT "A bacterial transcription terminator with inefficient molecular motor
RT action but with a robust transcription termination function.";
RL J. Mol. Biol. 395:966-982(2010).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Participates in transcription elongation, termination and
CC antitermination. {ECO:0000255|HAMAP-Rule:MF_00948}.
CC -!- SUBUNIT: Does not interact with Rho.
CC -!- SIMILARITY: Belongs to the NusG family. {ECO:0000255|HAMAP-
CC Rule:MF_00948}.
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DR EMBL; AL123456; CCP43382.1; -; Genomic_DNA.
DR PIR; D70613; D70613.
DR RefSeq; NP_215153.1; NC_000962.3.
DR RefSeq; WP_003403288.1; NZ_NVQJ01000007.1.
DR PDB; 2MI6; NMR; -; A=178-238.
DR PDBsum; 2MI6; -.
DR AlphaFoldDB; P9WIU9; -.
DR BMRB; P9WIU9; -.
DR SMR; P9WIU9; -.
DR STRING; 83332.Rv0639; -.
DR PaxDb; P9WIU9; -.
DR DNASU; 888039; -.
DR GeneID; 45424599; -.
DR GeneID; 888039; -.
DR KEGG; mtu:Rv0639; -.
DR TubercuList; Rv0639; -.
DR eggNOG; COG0250; Bacteria.
DR OMA; DGPFNGF; -.
DR PhylomeDB; P9WIU9; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0006354; P:DNA-templated transcription, elongation; IEA:UniProtKB-UniRule.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 3.30.70.940; -; 1.
DR HAMAP; MF_00948; NusG; 1.
DR InterPro; IPR006645; NGN_dom.
DR InterPro; IPR036735; NGN_dom_sf.
DR InterPro; IPR043425; NusG-like.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR001062; Transcrpt_antiterm_NusG.
DR InterPro; IPR015869; Transcrpt_antiterm_NusG_bac_CS.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR30265; PTHR30265; 1.
DR PANTHER; PTHR30265:SF2; PTHR30265:SF2; 1.
DR Pfam; PF02357; NusG; 1.
DR PRINTS; PR00338; NUSGTNSCPFCT.
DR SMART; SM00738; NGN; 1.
DR SUPFAM; SSF50104; SSF50104; 1.
DR SUPFAM; SSF82679; SSF82679; 1.
DR TIGRFAMs; TIGR00922; nusG; 1.
DR PROSITE; PS01014; NUSG; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Transcription;
KW Transcription antitermination; Transcription regulation;
KW Transcription termination.
FT CHAIN 1..238
FT /note="Transcription termination/antitermination protein
FT NusG"
FT /id="PRO_0000113936"
FT STRAND 190..193
FT /evidence="ECO:0007829|PDB:2MI6"
FT STRAND 200..209
FT /evidence="ECO:0007829|PDB:2MI6"
FT TURN 210..213
FT /evidence="ECO:0007829|PDB:2MI6"
FT STRAND 214..219
FT /evidence="ECO:0007829|PDB:2MI6"
FT TURN 222..224
FT /evidence="ECO:0007829|PDB:2MI6"
FT STRAND 226..231
FT /evidence="ECO:0007829|PDB:2MI6"
FT STRAND 234..237
FT /evidence="ECO:0007829|PDB:2MI6"
SQ SEQUENCE 238 AA; 25447 MW; E083F9754E42144C CRC64;
MTTFDGDTSA GEAVDLTEAN AFQDAAAPAE EVDPAAALKA ELRSKPGDWY VVHSYAGYEN
KVKANLETRV QNLDVGDYIF QVEVPTEEVT EIKNGQRKQV NRKVLPGYIL VRMDLTDDSW
AAVRNTPGVT GFVGATSRPS ALALDDVVKF LLPRGSTRKA AKGAASTAAA AEAGGLERPV
VEVDYEVGES VTVMDGPFAT LPATISEVNA EQQKLKVLVS IFGRETPVEL TFGQVSKI
