ID   NUSG_SHIFL              Reviewed;         181 AA.
AC   P0AFG2; P16921;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Transcription termination/antitermination protein NusG {ECO:0000255|HAMAP-Rule:MF_00948};
GN   Name=nusG {ECO:0000255|HAMAP-Rule:MF_00948};
GN   OrderedLocusNames=SF4055, S3680;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Participates in transcription elongation, termination and
CC       antitermination. In the absence of Rho, increases the rate of
CC       transcription elongation by the RNA polymerase (RNAP), probably by
CC       partially suppressing pausing. In the presence of Rho, modulates most
CC       Rho-dependent termination events by interacting with the RNAP to render
CC       the complex more susceptible to the termination activity of Rho. May be
CC       required to overcome a kinetic limitation of Rho to function at certain
CC       terminators. Also involved in ribosomal RNA transcriptional
CC       antitermination. {ECO:0000255|HAMAP-Rule:MF_00948}.
CC   -!- SUBUNIT: Monomer. Interacts with the transcription termination factor
CC       Rho and with RNA polymerase. {ECO:0000255|HAMAP-Rule:MF_00948}.
CC   -!- SIMILARITY: Belongs to the NusG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00948}.
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DR   EMBL; AE005674; AAN45484.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP18717.1; -; Genomic_DNA.
DR   RefSeq; NP_709777.1; NC_004337.2.
DR   RefSeq; WP_001287516.1; NZ_WPGW01000040.1.
DR   AlphaFoldDB; P0AFG2; -.
DR   SMR; P0AFG2; -.
DR   STRING; 198214.SF4055; -.
DR   EnsemblBacteria; AAN45484; AAN45484; SF4055.
DR   EnsemblBacteria; AAP18717; AAP18717; S3680.
DR   GeneID; 1025388; -.
DR   GeneID; 66672107; -.
DR   KEGG; sfl:SF4055; -.
DR   KEGG; sfx:S3680; -.
DR   PATRIC; fig|198214.7.peg.4778; -.
DR   HOGENOM; CLU_067287_1_0_6; -.
DR   OMA; DGPFNGF; -.
DR   OrthoDB; 2027986at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:UniProt.
DR   GO; GO:0006354; P:DNA-templated transcription, elongation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR   GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR   GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.30.30.30; -; 1.
DR   Gene3D; 3.30.70.940; -; 1.
DR   HAMAP; MF_00948; NusG; 1.
DR   InterPro; IPR005824; KOW.
DR   InterPro; IPR006645; NGN_dom.
DR   InterPro; IPR036735; NGN_dom_sf.
DR   InterPro; IPR043425; NusG-like.
DR   InterPro; IPR014722; Rib_L2_dom2.
DR   InterPro; IPR001062; Transcrpt_antiterm_NusG.
DR   InterPro; IPR015869; Transcrpt_antiterm_NusG_bac_CS.
DR   InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR   PANTHER; PTHR30265; PTHR30265; 1.
DR   PANTHER; PTHR30265:SF2; PTHR30265:SF2; 1.
DR   Pfam; PF00467; KOW; 1.
DR   Pfam; PF02357; NusG; 1.
DR   PRINTS; PR00338; NUSGTNSCPFCT.
DR   SMART; SM00739; KOW; 1.
DR   SMART; SM00738; NGN; 1.
DR   SUPFAM; SSF50104; SSF50104; 1.
DR   SUPFAM; SSF82679; SSF82679; 1.
DR   TIGRFAMs; TIGR00922; nusG; 1.
DR   PROSITE; PS01014; NUSG; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Transcription; Transcription antitermination;
KW   Transcription regulation; Transcription termination.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..181
FT                   /note="Transcription termination/antitermination protein
FT                   NusG"
FT                   /id="PRO_0000113945"
FT   DOMAIN          130..161
FT                   /note="KOW"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00948"
SQ   SEQUENCE   181 AA;  20532 MW;  B3AF94284CC17B04 CRC64;
     MSEAPKKRWY VVQAFSGFEG RVATSLREHI KLHNMEDLFG EVMVPTEEVV EIRGGQRRKS
     ERKFFPGYVL VQMVMNDASW HLVRSVPRVM GFIGGTSDRP APISDKEVDA IMNRLQQVGD
     KPRPKTLFEP GEMVRVNDGP FADFNGVVEE VDYEKSRLKV SVSIFGRATP VELDFSQVEK
     A