ID   NUSG_STRVG              Reviewed;         299 AA.
AC   P27309;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Transcription termination/antitermination protein NusG {ECO:0000255|HAMAP-Rule:MF_00948};
DE   AltName: Full=Butanolide receptor;
GN   Name=nusG {ECO:0000255|HAMAP-Rule:MF_00948}; Synonyms=vbrA;
OS   Streptomyces virginiae.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces virginiae group.
OX   NCBI_TaxID=1961;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=MAFF10-06014;
RX   PubMed=1309760; DOI=10.1016/s0021-9258(18)48400-1;
RA   Okamoto S., Nihirra T., Kataoka H., Suzuki A., Yamada Y.;
RT   "Purification and molecular cloning of a butyrolactone autoregulator
RT   receptor from Streptomyces virginiae.";
RL   J. Biol. Chem. 267:1093-1098(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8675024; DOI=10.1016/0378-1119(96)00067-4;
RA   Katayama M., Sakai Y., Okamoto S., Ihara F., Nihira T., Yamada Y.;
RT   "Gene organization in the ada-rplL region of Streptomyces virginiae.";
RL   Gene 171:135-136(1996).
CC   -!- FUNCTION: Participates in transcription elongation, termination and
CC       antitermination. {ECO:0000255|HAMAP-Rule:MF_00948}.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the NusG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00948}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA01261.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; D10468; BAA01261.1; ALT_INIT; Genomic_DNA.
DR   EMBL; D50624; BAA09301.1; -; Genomic_DNA.
DR   PIR; A41773; A41773.
DR   RefSeq; WP_033225042.1; NZ_JNYC01000038.1.
DR   AlphaFoldDB; P27309; -.
DR   SMR; P27309; -.
DR   GO; GO:0006354; P:DNA-templated transcription, elongation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR   GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR   GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.30.30.30; -; 1.
DR   Gene3D; 3.30.70.940; -; 1.
DR   HAMAP; MF_00948; NusG; 1.
DR   InterPro; IPR005824; KOW.
DR   InterPro; IPR006645; NGN_dom.
DR   InterPro; IPR036735; NGN_dom_sf.
DR   InterPro; IPR043425; NusG-like.
DR   InterPro; IPR014722; Rib_L2_dom2.
DR   InterPro; IPR001062; Transcrpt_antiterm_NusG.
DR   InterPro; IPR015869; Transcrpt_antiterm_NusG_bac_CS.
DR   InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR   PANTHER; PTHR30265; PTHR30265; 1.
DR   PANTHER; PTHR30265:SF2; PTHR30265:SF2; 1.
DR   Pfam; PF02357; NusG; 1.
DR   PRINTS; PR00338; NUSGTNSCPFCT.
DR   SMART; SM00739; KOW; 1.
DR   SMART; SM00738; NGN; 1.
DR   SUPFAM; SSF50104; SSF50104; 1.
DR   SUPFAM; SSF82679; SSF82679; 1.
DR   TIGRFAMs; TIGR00922; nusG; 1.
DR   PROSITE; PS01014; NUSG; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Repeat; Transcription;
KW   Transcription antitermination; Transcription regulation;
KW   Transcription termination.
FT   CHAIN           1..299
FT                   /note="Transcription termination/antitermination protein
FT                   NusG"
FT                   /id="PRO_0000113963"
FT   REPEAT          46..49
FT                   /note="1"
FT   REPEAT          70..73
FT                   /note="2"
FT   REPEAT          80..83
FT                   /note="3; approximate"
FT   REPEAT          84..87
FT                   /note="4"
FT   DOMAIN          248..276
FT                   /note="KOW"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00948"
FT   REGION          30..96
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          46..87
FT                   /note="4 X 4 AA repeats of E-E-A-A"
FT   COMPBIAS        51..89
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   299 AA;  32592 MW;  3897260A48EDAC0B CRC64;
     MSDPNLNASH DSVESVEDEL DIVEAADAVD PDEAELADAE AGAPAEEAAL HVESDEDEDE
     ADVEVDAAVE EAADDAEVAE EEAEEAAPVE PAEPVDPIQA LREELRLLPG EWYVIHTYAG
     YEKRVKANLE QRAVSLNVEE FIYQAEVPEE EIVQIKNGER KNVRQNKLPG YVLVRMDLTN
     ESWGVVRNTP GVTGFVGNAY DPYPLTLDEI VKMLAPEAQE KAAKAAAEEA GLPAPAVKRT
     IEVLDFEVGD SVTVTDGPFA TLQATINEIN PDSKKVKGLV EIFGRETPVE LSFDQIQKN