NUSG_THET8
ID NUSG_THET8 Reviewed; 184 AA.
AC P35872; Q5SLP5;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=Transcription termination/antitermination protein NusG {ECO:0000255|HAMAP-Rule:MF_00948};
GN Name=nusG {ECO:0000255|HAMAP-Rule:MF_00948}; OrderedLocusNames=TTHA0248;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RX PubMed=1447157; DOI=10.1128/jb.174.23.7859-7863.1992;
RA Heinrich T., Schroeder W., Erdmann V.A., Hartmann R.K.;
RT "Identification of the gene encoding transcription factor NusG of Thermus
RT thermophilus.";
RL J. Bacteriol. 174:7859-7863(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP STRUCTURE BY NMR OF 2-120.
RX PubMed=15162485; DOI=10.1002/prot.20054;
RA Reay P., Yamasaki K., Terada T., Kuramitsu S., Shirouzu M., Yokoyama S.;
RT "Structural and sequence comparisons arising from the solution structure of
RT the transcription elongation factor NusG from Thermus thermophilus.";
RL Proteins 56:40-51(2004).
CC -!- FUNCTION: Participates in transcription elongation, termination and
CC antitermination. {ECO:0000255|HAMAP-Rule:MF_00948}.
CC -!- SIMILARITY: Belongs to the NusG family. {ECO:0000255|HAMAP-
CC Rule:MF_00948}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA27500.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; L10348; AAA27500.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP008226; BAD70071.1; -; Genomic_DNA.
DR RefSeq; WP_008630512.1; NC_006461.1.
DR RefSeq; YP_143514.1; NC_006461.1.
DR PDB; 1NZ8; NMR; -; A=2-120.
DR PDB; 1NZ9; NMR; -; A=127-184.
DR PDBsum; 1NZ8; -.
DR PDBsum; 1NZ9; -.
DR AlphaFoldDB; P35872; -.
DR SMR; P35872; -.
DR STRING; 300852.55771630; -.
DR EnsemblBacteria; BAD70071; BAD70071; BAD70071.
DR GeneID; 3168047; -.
DR KEGG; ttj:TTHA0248; -.
DR PATRIC; fig|300852.9.peg.248; -.
DR eggNOG; COG0250; Bacteria.
DR HOGENOM; CLU_067287_1_1_0; -.
DR OMA; DGPFNGF; -.
DR PhylomeDB; P35872; -.
DR EvolutionaryTrace; P35872; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0006354; P:DNA-templated transcription, elongation; IEA:UniProtKB-UniRule.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 3.30.70.940; -; 1.
DR HAMAP; MF_00948; NusG; 1.
DR InterPro; IPR005824; KOW.
DR InterPro; IPR006645; NGN_dom.
DR InterPro; IPR036735; NGN_dom_sf.
DR InterPro; IPR043425; NusG-like.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR001062; Transcrpt_antiterm_NusG.
DR InterPro; IPR015869; Transcrpt_antiterm_NusG_bac_CS.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR30265; PTHR30265; 1.
DR PANTHER; PTHR30265:SF2; PTHR30265:SF2; 1.
DR Pfam; PF00467; KOW; 1.
DR Pfam; PF02357; NusG; 1.
DR PRINTS; PR00338; NUSGTNSCPFCT.
DR SMART; SM00739; KOW; 1.
DR SMART; SM00738; NGN; 1.
DR SUPFAM; SSF50104; SSF50104; 1.
DR SUPFAM; SSF82679; SSF82679; 1.
DR TIGRFAMs; TIGR00922; nusG; 1.
DR PROSITE; PS01014; NUSG; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Transcription;
KW Transcription antitermination; Transcription regulation;
KW Transcription termination.
FT CHAIN 1..184
FT /note="Transcription termination/antitermination protein
FT NusG"
FT /id="PRO_0000113966"
FT DOMAIN 133..163
FT /note="KOW"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00948"
FT STRAND 4..9
FT /evidence="ECO:0007829|PDB:1NZ8"
FT TURN 12..14
FT /evidence="ECO:0007829|PDB:1NZ8"
FT HELIX 15..29
FT /evidence="ECO:0007829|PDB:1NZ8"
FT TURN 32..34
FT /evidence="ECO:0007829|PDB:1NZ8"
FT STRAND 37..39
FT /evidence="ECO:0007829|PDB:1NZ8"
FT STRAND 41..47
FT /evidence="ECO:0007829|PDB:1NZ8"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:1NZ8"
FT STRAND 55..62
FT /evidence="ECO:0007829|PDB:1NZ8"
FT STRAND 65..70
FT /evidence="ECO:0007829|PDB:1NZ8"
FT STRAND 74..77
FT /evidence="ECO:0007829|PDB:1NZ8"
FT HELIX 80..87
FT /evidence="ECO:0007829|PDB:1NZ8"
FT STRAND 97..101
FT /evidence="ECO:0007829|PDB:1NZ8"
FT HELIX 106..116
FT /evidence="ECO:0007829|PDB:1NZ8"
FT STRAND 136..139
FT /evidence="ECO:0007829|PDB:1NZ9"
FT HELIX 142..144
FT /evidence="ECO:0007829|PDB:1NZ9"
FT STRAND 148..155
FT /evidence="ECO:0007829|PDB:1NZ9"
FT TURN 156..159
FT /evidence="ECO:0007829|PDB:1NZ9"
FT STRAND 160..176
FT /evidence="ECO:0007829|PDB:1NZ9"
FT HELIX 178..180
FT /evidence="ECO:0007829|PDB:1NZ9"
FT STRAND 181..183
FT /evidence="ECO:0007829|PDB:1NZ9"
SQ SEQUENCE 184 AA; 20449 MW; 1F9406B9EFDF9C0E CRC64;
MSIEWYAVHT LVGQEEKAKA NLEKRIKAFG LQDKIFQVLI PTEEVVELRE GGKKEVVRKK
LFPGYLFIQM DLGDEEEPNE AWEVVRGTPG ITGFVGAGMR PVPLSPDEVR HILEVSGLLG
KKEAPKAQVA FREGDQVRVV SGPFADFTGT VTEINPERGK VKVMVTIFGR ETPVELDFSQ
VVKA
