ID   NUXM_NEUCR              Reviewed;         189 AA.
AC   Q02854; Q7SHE7;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 111.
DE   RecName: Full=NADH-ubiquinone oxidoreductase 20.9 kDa subunit;
DE            EC=7.1.1.2;
DE   AltName: Full=Complex I-20.9kD;
DE            Short=CI-20.9kD;
GN   Name=nuo20.9; ORFNames=NCU01859;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 138-155.
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=1445273; DOI=10.1042/bj2880029;
RA   Azevedo J.E., Nehls U., Eckerskorn C., Heinrich H., Rothe H., Weiss H.,
RA   Werner S.;
RT   "Primary structure and mitochondrial import in vitro of the 20.9 kDa
RT   subunit of complex I from Neurospora crassa.";
RL   Biochem. J. 288:29-34(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Transfer of electrons from NADH to the respiratory chain. The
CC       immediate electron acceptor for the enzyme is believed to be
CC       ubiquinone.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBUNIT: Complex I is composed of about 40 different subunits.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC       Single-pass membrane protein {ECO:0000305}.
CC   -!- PTM: The N-terminus is blocked.
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DR   EMBL; X60829; CAA43221.1; -; mRNA.
DR   EMBL; CM002236; EAA36254.3; -; Genomic_DNA.
DR   PIR; S27171; S27171.
DR   RefSeq; XP_965490.3; XM_960397.3.
DR   AlphaFoldDB; Q02854; -.
DR   SMR; Q02854; -.
DR   STRING; 5141.EFNCRP00000001025; -.
DR   TCDB; 3.D.1.6.2; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR   EnsemblFungi; EAA36254; EAA36254; NCU01859.
DR   GeneID; 3881668; -.
DR   KEGG; ncr:NCU01859; -.
DR   VEuPathDB; FungiDB:NCU01859; -.
DR   HOGENOM; CLU_087364_0_0_1; -.
DR   Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR   GO; GO:0005747; C:mitochondrial respiratory chain complex I; IBA:GO_Central.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   InterPro; IPR024549; NADH-UbQ_OxRdtase_su21_C_fun.
DR   InterPro; IPR019721; NADH-UbQ_OxRdtase_su21_N.
DR   Pfam; PF10785; NADH-u_ox-rdase; 1.
DR   Pfam; PF12853; NADH_u_ox_C; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; NAD; Oxidoreductase; Reference proteome;
KW   Translocase; Transmembrane; Transmembrane helix; Ubiquinone.
FT   CHAIN           1..189
FT                   /note="NADH-ubiquinone oxidoreductase 20.9 kDa subunit"
FT                   /id="PRO_0000118782"
FT   TRANSMEM        73..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   189 AA;  20977 MW;  E3F23FD959092EC1 CRC64;
     MSSTSSPTYT ISKTLNTNYP LIDNDPHFRR VIGYARPSDY VHGTVAGAAG PGLLYLMEKM
     APSGVGKGGF PKAMRLATAV GFFGGFLYFY QRSILRFYGM SENAREVQMD MREMVDKVKA
     GQPLYGVSTL PVDVQGMAAR QSRYSALFFA VLPWFNFVNH NQHGVDTAKY YQQAERELEA
     ERLGKGSSS