ID   NV4_NEMVE               Reviewed;          81 AA.
AC   A0A5J6K6K4;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   11-DEC-2019, sequence version 1.
DT   03-AUG-2022, entry version 5.
DE   RecName: Full=N.vectensis toxin 4 {ECO:0000305};
DE            Short=Nv4 {ECO:0000303|PubMed:31134275};
DE   Flags: Precursor;
OS   Nematostella vectensis (Starlet sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Edwardsiidae; Nematostella.
OX   NCBI_TaxID=45351;
RN   [1] {ECO:0000312|EMBL:QEV81587.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY,
RP   DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, AND RECOMBINANT EXPRESSION.
RX   PubMed=31134275; DOI=10.1093/molbev/msz132;
RA   Sachkova M.Y., Singer S.A., Macrander J., Reitzel A.M., Peigneur S.,
RA   Tytgat J., Moran Y.;
RT   "The birth and death of toxins with distinct functions: a case study in the
RT   sea anemone Nematostella.";
RL   Mol. Biol. Evol. 36:2001-2012(2019).
CC   -!- FUNCTION: Has toxic effects on zebrafish larvae (PubMed:31134275). It
CC       causes contractile paralysis and twitching of the tail within 30
CC       minutes, followed by death within 40 minutes (PubMed:31134275). Does
CC       not show any toxicity when injected into arthropods (cherry shrimps or
CC       grass shrimps) (PubMed:31134275). {ECO:0000269|PubMed:31134275}.
CC   -!- TISSUE SPECIFICITY: Expressed in ectodermal gland cells
CC       (PubMed:31134275). In adult female tissues, highly transcribed in
CC       mesenteries (gametes-producing tissue) and slightly transcribed in
CC       tentacles, pharynx and physa (PubMed:31134275).
CC       {ECO:0000269|PubMed:31134275}.
CC   -!- DEVELOPMENTAL STAGE: Is detected in unfertilized eggs, late planulae,
CC       and primary polyps, but not in adults (both males and females) (at
CC       protein level). At mRNA level, is found at multiple developmental
CC       stages including unfertilized eggs, blastulae, gastrulae, early
CC       planulae, planulae, metamorphosing planulae, primary polyps, juvenile
CC       polyps (low levels at 4 months old, but no mRNA at 2 months), adult
CC       males, and adult females, with the highest levels from gastrulae to
CC       primary polyps, and adult females. {ECO:0000269|PubMed:31134275}.
CC   -!- MISCELLANEOUS: Does not show activity on all ion channel tested at 10
CC       uM (hNav1.1/SCN1A, rNav1.2/SCN2A, rNav1.3/SCN3A, rNav1.4/SCN4A,
CC       hNav1.5/SCN5A, mNav1.6/SCN8A, rNav1.8/SCN10A, BgNav1, Shaker IR,
CC       rKv1.1/KCNA1, rKv1.2/KCNA2, hKv1.3/KCNA3, rKv1.4/KCNA4, rKv1.5/KCNA5,
CC       rKv1.6/KCNA6, rKv2.1/KCNB1, hKv3.1/KCNC1, rKv4.3/KCND3, hKv7.2/KCNQ2,
CC       hKv7.3/KCNQ3, hKv10.1/KCNH1/EAG1, hKv11.1/KCNH2/ERG1).
CC       {ECO:0000269|PubMed:31134275}.
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DR   EMBL; MK608361; QEV81587.1; -; mRNA.
DR   SMR; A0A5J6K6K4; -.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Disulfide bond; Neurotoxin; Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..81
FT                   /note="N.vectensis toxin 4"
FT                   /evidence="ECO:0000305|PubMed:31134275"
FT                   /id="PRO_5023842426"
FT   DISULFID        46..75
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
FT   DISULFID        48..70
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
FT   DISULFID        63..76
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
SQ   SEQUENCE   81 AA;  9219 MW;  F41A1F62FD50B03B CRC64;
     MRSSWMFVIC FAMLILYTNG RILRPETSWE DELEDDLANN KRSLACSCED GSGRTGTHWI
     FDCPKGWADC GGFRCCVETS K