ID   NV5_NEMVE               Reviewed;          84 AA.
AC   A0A5J6KCM0;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   11-DEC-2019, sequence version 1.
DT   23-FEB-2022, entry version 4.
DE   RecName: Full=N.vectensis toxin 5 {ECO:0000305};
DE            Short=Nv5 {ECO:0000303|PubMed:31134275};
DE   Flags: Precursor;
OS   Nematostella vectensis (Starlet sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Edwardsiidae; Nematostella.
OX   NCBI_TaxID=45351;
RN   [1] {ECO:0000312|EMBL:QEV81588.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY,
RP   DEVELOPMENTAL STAGE, AND RECOMBINANT EXPRESSION.
RX   PubMed=31134275; DOI=10.1093/molbev/msz132;
RA   Sachkova M.Y., Singer S.A., Macrander J., Reitzel A.M., Peigneur S.,
RA   Tytgat J., Moran Y.;
RT   "The birth and death of toxins with distinct functions: a case study in the
RT   sea anemone Nematostella.";
RL   Mol. Biol. Evol. 36:2001-2012(2019).
CC   -!- FUNCTION: Has toxic effects on zebrafish larvae (PubMed:31134275). It
CC       causes contractile paralysis and twitching of the tail within 20
CC       minutes, followed by death within 30 minutes (PubMed:31134275). Does
CC       not show any toxicity when injected into arthropods (cherry shrimps or
CC       grass shrimps) (PubMed:31134275). {ECO:0000269|PubMed:31134275}.
CC   -!- TISSUE SPECIFICITY: Expressed in ectodermal gland cells
CC       (PubMed:31134275). In adult female tissues, highly transcribed in
CC       mesenteries (gametes-producing tissue) and slightly transcribed in
CC       tentacles, pharynx and physa (PubMed:31134275).
CC       {ECO:0000269|PubMed:31134275}.
CC   -!- DEVELOPMENTAL STAGE: Is detected in unfertilized eggs (at protein
CC       level). At mRNA level, its highest expression level is observed in the
CC       unfertilized eggs. Starting from blastula stage, its expression
CC       gradually drops (in gastrulae, early planulae, planulae, metamorphosing
CC       planulae, primary polyps, and juvenile polyps (2 and 4 months old))
CC       till the metamorphosis stage and peaks again in the adult females (a
CC       small increase is observed in adult males).
CC       {ECO:0000269|PubMed:31134275}.
CC   -!- MISCELLANEOUS: Does not show activity on all ion channel tested at 10
CC       uM (hNav1.1/SCN1A, rNav1.2/SCN2A, rNav1.3/SCN3A, rNav1.4/SCN4A,
CC       hNav1.5/SCN5A, mNav1.6/SCN8A, rNav1.8/SCN10A, BgNav1, Shaker IR,
CC       rKv1.1/KCNA1, rKv1.2/KCNA2, hKv1.3/KCNA3, rKv1.4/KCNA4, rKv1.5/KCNA5,
CC       rKv1.6/KCNA6, rKv2.1/KCNB1, hKv3.1/KCNC1, rKv4.3/KCND3, hKv7.2/KCNQ2,
CC       hKv7.3/KCNQ3, hKv10.1/KCNH1/EAG1, hKv11.1/KCNH2/ERG1).
CC       {ECO:0000269|PubMed:31134275}.
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DR   EMBL; MK608362; QEV81588.1; -; mRNA.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Disulfide bond; Neurotoxin; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..84
FT                   /note="N.vectensis toxin 5"
FT                   /evidence="ECO:0000305|PubMed:31134275"
FT                   /id="PRO_5023909572"
FT   DISULFID        45..77
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
FT   DISULFID        47..68
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
FT   DISULFID        61..78
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
SQ   SEQUENCE   84 AA;  9357 MW;  0E9ECC2B94AB7EAF CRC64;
     MNSLLKVAVV CLVMLVACSS GRVVLPDDAD TEYNSEYALA KRVNCKCEGG HEEGTYWVFG
     CPKNWYSCKS GYVYGRCCSQ QWGK