ID   NV8_NEMVE               Reviewed;          91 AA.
AC   P0DQR4;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   03-AUG-2022, entry version 3.
DE   RecName: Full=N.vectensis toxin 8 {ECO:0000305};
DE            Short=Nv8 {ECO:0000303|PubMed:31134275};
DE   Flags: Precursor;
OS   Nematostella vectensis (Starlet sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Edwardsiidae; Nematostella.
OX   NCBI_TaxID=45351;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=31134275; DOI=10.1093/molbev/msz132;
RA   Sachkova M.Y., Singer S.A., Macrander J., Reitzel A.M., Peigneur S.,
RA   Tytgat J., Moran Y.;
RT   "The birth and death of toxins with distinct functions: a case study in the
RT   sea anemone Nematostella.";
RL   Mol. Biol. Evol. 36:2001-2012(2019).
CC   -!- FUNCTION: Has toxic effects on zebrafish larvae. It causes contractile
CC       paralysis and twitching of the tail within 20 minutes, followed by
CC       death within 30 minutes. Does not show any toxicity when injected into
CC       arthropods (cherry shrimps or grass shrimps).
CC       {ECO:0000250|UniProtKB:A0A5J6KCM0}.
CC   -!- TISSUE SPECIFICITY: Expressed in ectodermal gland cells.
CC       {ECO:0000250|UniProtKB:A0A5J6KCM0}.
CC   -!- DEVELOPMENTAL STAGE: No protein detected at any developmental stage,
CC       probably due to low expression levels (PubMed:31134275). At
CC       transcriptional level, is found in the larval stage (PubMed:31134275).
CC       {ECO:0000269|PubMed:31134275}.
CC   -!- MISCELLANEOUS: Does not show activity on all voltage-gated potassium
CC       and sodium channels. {ECO:0000250|UniProtKB:A0A5J6KCM0}.
CC   -!- WEB RESOURCE: Name=National Center for Biotechnology Information
CC       (NCBI);
CC       URL="https://www.ncbi.nlm.nih.gov/nucleotide/XR_004292627.1?report=genbank&log$=nuclalign&blast_rank=1&RID=CTDEH2MX013&from=477&to=683";
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DR   SMR; P0DQR4; -.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Neurotoxin; Signal; Toxin.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..91
FT                   /note="N.vectensis toxin 8"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000453819"
FT   DISULFID        45..76
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
FT   DISULFID        47..67
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
FT   DISULFID        60..77
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
SQ   SEQUENCE   91 AA;  10259 MW;  8ADA65A95C7D6938 CRC64;
     MNSLLKVAVV CLVMLVACFV PRVILTDDAD TEYNSDYLLP KRAGCKCADG RQETYYFMGC
     QGGWYRCSPH YVIGDCCKKF SGKYGTFAVR F