NWD1_MOUSE
ID NWD1_MOUSE Reviewed; 1563 AA.
AC A6H603; Q3TRC9; Q8BS99;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=NACHT domain- and WD repeat-containing protein 1;
GN Name=Nwd1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Hypothalamus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 38-1563 (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May play a role in the control of androgen receptor (AR)
CC protein steady-state levels. {ECO:0000250|UniProtKB:Q149M9}.
CC -!- SUBUNIT: May interact with HSP90AA1, HSP90AB1 and BAG2.
CC {ECO:0000250|UniProtKB:Q149M9}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:Q149M9}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=A6H603-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A6H603-2; Sequence=VSP_028945, VSP_028946;
CC Name=3;
CC IsoId=A6H603-3; Sequence=VSP_028942, VSP_028945, VSP_028946;
CC Name=4;
CC IsoId=A6H603-4; Sequence=VSP_028943, VSP_028944;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI45704.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAE37101.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK034868; BAC28860.1; -; mRNA.
DR EMBL; AK162888; BAE37101.1; ALT_FRAME; mRNA.
DR EMBL; AC171917; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC145703; AAI45704.1; ALT_INIT; mRNA.
DR RefSeq; XP_006531150.1; XM_006531087.3. [A6H603-1]
DR AlphaFoldDB; A6H603; -.
DR STRING; 10090.ENSMUSP00000124804; -.
DR iPTMnet; A6H603; -.
DR PhosphoSitePlus; A6H603; -.
DR PaxDb; A6H603; -.
DR PeptideAtlas; A6H603; -.
DR PRIDE; A6H603; -.
DR ProteomicsDB; 293787; -. [A6H603-1]
DR ProteomicsDB; 293788; -. [A6H603-2]
DR ProteomicsDB; 293789; -. [A6H603-3]
DR ProteomicsDB; 293790; -. [A6H603-4]
DR Antibodypedia; 69163; 8 antibodies from 7 providers.
DR Ensembl; ENSMUST00000093427; ENSMUSP00000091135; ENSMUSG00000048148. [A6H603-1]
DR GeneID; 319555; -.
DR UCSC; uc012gfx.1; mouse. [A6H603-3]
DR CTD; 284434; -.
DR MGI; MGI:2442268; Nwd1.
DR VEuPathDB; HostDB:ENSMUSG00000048148; -.
DR eggNOG; KOG0266; Eukaryota.
DR eggNOG; KOG3602; Eukaryota.
DR GeneTree; ENSGT00940000161635; -.
DR InParanoid; A6H603; -.
DR OrthoDB; 20367at2759; -.
DR PhylomeDB; A6H603; -.
DR BioGRID-ORCS; 319555; 6 hits in 71 CRISPR screens.
DR ChiTaRS; Nwd1; mouse.
DR PRO; PR:A6H603; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; A6H603; protein.
DR Bgee; ENSMUSG00000048148; Expressed in olfactory epithelium and 73 other tissues.
DR ExpressionAtlas; A6H603; baseline and differential.
DR Genevisible; A6H603; MM.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 3.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR024977; Apc4_WD40_dom.
DR InterPro; IPR007111; NACHT_NTPase.
DR InterPro; IPR043365; NWD1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR45013; PTHR45013; 1.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF05729; NACHT; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 10.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Cytoplasm; Nucleotide-binding;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..1563
FT /note="NACHT domain- and WD repeat-containing protein 1"
FT /id="PRO_0000308255"
FT REPEAT 274..314
FT /note="WD 1"
FT DOMAIN 336..666
FT /note="NACHT"
FT REPEAT 866..905
FT /note="WD 2"
FT REPEAT 908..947
FT /note="WD 3"
FT REPEAT 954..994
FT /note="WD 4"
FT REPEAT 998..1037
FT /note="WD 5"
FT REPEAT 1044..1082
FT /note="WD 6"
FT REPEAT 1126..1165
FT /note="WD 7"
FT REPEAT 1168..1207
FT /note="WD 8"
FT REPEAT 1212..1251
FT /note="WD 9"
FT REPEAT 1253..1292
FT /note="WD 10"
FT REPEAT 1346..1385
FT /note="WD 11"
FT REPEAT 1386..1425
FT /note="WD 12"
FT REPEAT 1431..1470
FT /note="WD 13"
FT REGION 1534..1563
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 342..349
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..207
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028942"
FT VAR_SEQ 911
FT /note="A -> V (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028943"
FT VAR_SEQ 912..1563
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028944"
FT VAR_SEQ 1430..1435
FT /note="NSCCRG -> VGGLDN (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_028945"
FT VAR_SEQ 1436..1563
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_028946"
FT CONFLICT 213
FT /note="V -> A (in Ref. 3; AAI45704)"
FT /evidence="ECO:0000305"
FT CONFLICT 218
FT /note="D -> G (in Ref. 1; BAC28860)"
FT /evidence="ECO:0000305"
FT CONFLICT 326
FT /note="Q -> R (in Ref. 3; AAI45704)"
FT /evidence="ECO:0000305"
FT CONFLICT 356
FT /note="A -> T (in Ref. 1; BAE37101)"
FT /evidence="ECO:0000305"
FT CONFLICT 410
FT /note="A -> V (in Ref. 3; AAI45704)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1563 AA; 173270 MW; 5FF4DA161866F3E4 CRC64;
MDTEREALQC TAYPEVQSFC QRHGLAFEVV DLRWGIPNTQ ATDYLTTELC LEELERCQKT
SIGPAFVALL GDQYGPCPVP RRIEEKEWEA LRAQLTSRPR DLELVTRHFQ RDDNTIPPTY
VLQPSGSLVV PGPEEATLTS VLRGGAQEAW RLGLISQEQW MCYHRSVIEW EIELGLLSSA
RGDQGATVFL RDVQDLNKHI LDDCSLKMVD RLVDGCLDTN AQSLLSGLKG RILDAQPGAL
KSHHLSWSRD LVNPKNKAHA RYLKQLSEQF VARTNHQVLE QLRELELARQ ELGWLYQEIR
HHLWQSTEST KVFCGHQELL AQLRQQLRQD ESRTHTPLVL FGPPGIGKTS LMCKLAQQVP
ELLGHKTVVV LRLLGTSKLS LDARSLLRSL SFQVCLAYGL PLPPAQVLEA HSRVGHFFHT
LLHTVSQRNF ESLVLLLDSV DDLDSICHSP RVSWLPLKCP PRVHLILSTC SGQQVLHNLQ
QTLKDPSTYW EVKALSGSQG QEFIQLLLAA EKRMLSPGQR DVLWASLPEC GHPGRLRLAF
EEARKWASFT VPVPLATTAE EATHQLCIRL EETHGALLVA HVLGYIVSSR YGLSEAELKD
VLSLDDEVLQ AVYRDWTPPS KELLRFPPLL WVRLRRDLGH CLVRRPVDGC MLLAIAHRQL
SQVIQVRYLS GPERAKRHGV LAEFFSGAWS QGIKKLITLP LVGKPLNLDR KVAPQPLWFS
STVANLRKLT ELPFHLLHAG RLEELKQEVL GNMSWISCRG ISGGIEVLLD DFDMCAPHMN
SPEVDLVREA IQLCGPAVEL RGLEKSVLYT ELLARLLFFA ASHPAMIGQL CQQAQSWFRA
CPYPMLVPLA GFLQPPGGPL RATLTGCHKG ITAIAWSLEE KLLVVGTQDG AMVVWDVEEQ
QVVHVLMGHT AEVKCVRVFA QGTLAISASK DHTLRLWSLL SGQEKVTILD GGSQNPTEPQ
SWDLHVDERN NVVYSTSGAR INMWNLETSK LVFCITGDVS DPWVCVALLA AQGLLLALSK
GGQVSLWSSA MGKLQEKHQL SSIKEETPTC AVSIQSRARL VAGFSSGSIA LVSAGEDRLL
EKLPEAVGFL VVSEDDSLLV AGFGRFVRIF LADSQGFHRF MASDLEHEDM VETAVLGPEN
NLIITGSRDA LIQVWSLSEQ GTLLNVLEGV GAPVSLLVRG GTLVVSASRK SSSFKVWDLK
STKKLQSPTP FLDRTGLAAV SHHGSFVYFP KVGDKNKVTI WDLAEGEEQD CLDTSNEVRC
LEVAEQAKLL FTGLVSGIVL VFPLNSRQDV LCIPPPEARK AVNCMSLSKS ENRLAIAYDN
IVLVLDISPG DPCPAIEGPT YTFYTQLPET IVSVAVLADY RVVYGMSDGS LFLYDCACSK
VFPLEAHGSR VSCVEVSHSE QLAVSGAEDA LLCLWDLQAC RGMFEMSYEN SCCRGVRCAC
FSRDDKHVFA GMEDRSVTAW STVDGTLLAV QFVHAVINRI IPTSNGFMAP TSHGYLIRER
FQCPSVRASQ QDPLKNFKKA VWLVKTRQRE ELAAAEASQD AEPVAVEGKE SKSNKRSQVC
LIL
