NWD2_MOUSE
ID NWD2_MOUSE Reviewed; 1742 AA.
AC Q6P5U7; Q3UVW7; Q8CHA7;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=NACHT and WD repeat domain-containing protein 2;
DE AltName: Full=Leucine-rich repeat and WD repeat-containing protein KIAA1239;
GN Name=Nwd2; Synonyms=Kiaa1239;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 482-1742.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 541-1742.
RX PubMed=12465718; DOI=10.1093/dnares/9.5.179;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: I.
RT The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 9:179-188(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1046-1742.
RC STRAIN=C57BL/6J; TISSUE=Diencephalon;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=16452087; DOI=10.1074/mcp.t500041-mcp200;
RA Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.;
RT "Comprehensive identification of phosphorylation sites in postsynaptic
RT density preparations.";
RL Mol. Cell. Proteomics 5:914-922(2006).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC41474.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC114408; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC122744; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC062655; AAH62655.1; -; mRNA.
DR EMBL; AB093290; BAC41474.1; ALT_INIT; mRNA.
DR EMBL; AK136869; BAE23152.1; -; mRNA.
DR CCDS; CCDS51508.1; -.
DR RefSeq; NP_795980.2; NM_177006.3.
DR AlphaFoldDB; Q6P5U7; -.
DR BioGRID; 235540; 3.
DR IntAct; Q6P5U7; 3.
DR MINT; Q6P5U7; -.
DR STRING; 10090.ENSMUSP00000124712; -.
DR iPTMnet; Q6P5U7; -.
DR PhosphoSitePlus; Q6P5U7; -.
DR MaxQB; Q6P5U7; -.
DR PaxDb; Q6P5U7; -.
DR PeptideAtlas; Q6P5U7; -.
DR PRIDE; Q6P5U7; -.
DR ProteomicsDB; 293909; -.
DR Antibodypedia; 71143; 3 antibodies from 3 providers.
DR Ensembl; ENSMUST00000159584; ENSMUSP00000124712; ENSMUSG00000090061.
DR GeneID; 319807; -.
DR KEGG; mmu:319807; -.
DR UCSC; uc008xmf.2; mouse.
DR CTD; 57495; -.
DR MGI; MGI:1920464; Nwd2.
DR VEuPathDB; HostDB:ENSMUSG00000090061; -.
DR eggNOG; KOG3602; Eukaryota.
DR GeneTree; ENSGT00940000153648; -.
DR HOGENOM; CLU_003267_0_0_1; -.
DR InParanoid; Q6P5U7; -.
DR OMA; PYIYIAR; -.
DR OrthoDB; 20367at2759; -.
DR PhylomeDB; Q6P5U7; -.
DR TreeFam; TF332647; -.
DR BioGRID-ORCS; 319807; 1 hit in 70 CRISPR screens.
DR ChiTaRS; Nwd2; mouse.
DR PRO; PR:Q6P5U7; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q6P5U7; protein.
DR Bgee; ENSMUSG00000090061; Expressed in habenula and 77 other tissues.
DR ExpressionAtlas; Q6P5U7; baseline and differential.
DR Genevisible; Q6P5U7; MM.
DR Gene3D; 2.130.10.10; -; 3.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 2.
DR SUPFAM; SSF50998; SSF50998; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Leucine-rich repeat; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..1742
FT /note="NACHT and WD repeat domain-containing protein 2"
FT /id="PRO_0000320921"
FT REPEAT 386..410
FT /note="LRR 1"
FT DOMAIN 410..737
FT /note="NACHT"
FT REPEAT 677..698
FT /note="LRR 2"
FT REPEAT 724..747
FT /note="LRR 3"
FT REPEAT 883..906
FT /note="LRR 4"
FT REPEAT 925..953
FT /note="LRR 5"
FT REPEAT 963..1004
FT /note="WD 1"
FT REPEAT 1007..1046
FT /note="WD 2"
FT REPEAT 1140..1179
FT /note="WD 3"
FT REPEAT 1229..1271
FT /note="WD 4"
FT REPEAT 1272..1311
FT /note="WD 5"
FT REPEAT 1314..1353
FT /note="WD 6"
FT REPEAT 1355..1394
FT /note="WD 7"
FT REPEAT 1396..1434
FT /note="WD 8"
FT REPEAT 1476..1516
FT /note="WD 9"
FT REPEAT 1522..1561
FT /note="WD 10"
FT REPEAT 1614..1653
FT /note="WD 11"
FT REGION 1702..1721
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1702..1718
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 1218
FT /note="G -> S (in Ref. 3; BAC41474)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1742 AA; 197414 MW; 630DA9B992EBB2D2 CRC64;
MWPAGAGTKL PCPRDSALRR AAFSGNLTAL PSHLVPAGRS VRVFISANPE DTGAERQALR
ETVYPKLREF CRENYGLEFQ VIDLYWGIEE DEWDSPELQK MRMKLLEECL KTSAGPCFVG
LLGEKYGNIR IPGEVEASEF EMILDAAVEA KLETKLLEDW YCRDENSVPA AYYLRPRLEV
PRSNKNSTQP SASSEQERPW QEISDEIKTI FKAAVKLLHE QGKMKQSQAK RYLFSAIEDE
FDFALGKQTP AFLKKCVCYI RKIANIERFV KIPEMGKYMD ITGTDPRIVR DPEAQEKLIK
LRDEFIPTIV ASSNLRVYTS VTHCDMKLGY SQEIENHYIE GLGKQFYEDM IDIIQATVQQ
NFDTETDTLY DEILQHSSLC KTYASFYEYK CESLNILHKY ILPSKTGHIN PLVVYGGPCT
GKTLLLAEVA KKAYGWLHED TGPDSDPVVI VRFLGTTDMS IDLRTLLLSV CEQLAVNYRC
LVQSFPKKIH DLRDLFINLL NESSLQRPLV IILDALEQLS EADEARKLWW LPAHLPRFVR
IILSTLPNKH GILQKLRCLI HEEDNYIELI PRDRKMCSQV LKHQLLRVKR KVTSGQQIYV
NNAFSKCTLP MFVNLTFREV RHWRSHKDVD ESSLCVTVHE SIEQLFWSLE KKCGQKLVSR
ALGYITMAKM GLSEMELEDV LALDNSVMNE LNENTRPSNP LRVPYLYIAR LKEGLNGYLI
ERHVKNVTLL VWANRHLQLI AQKLYLQEDS NLREMHTILA DYFLGVWSGG RRKAFCLEDP
YLNGCLDLEN RSLLEEEKHF MEQASFDRQA PDQPWVFQCN PLEPDIFFVN HRKMSELLYH
LTRCGKTDDL LYGIIMNFSW LYTMIKIGQF DKVLADIELA YNYSQEKELK FLASTLRSIR
NKVIAFPGSL SAELQQRLLP VVSSLPKLRH LLLECDKDGP KYCSIVPLHS SMDVTYSPER
LPLASSHLHV TEILPTCNPS TVLTALENGS ISTWDVETRQ LLRQITTAQS VILGMKLSSD
EKYLVVATTN NTLLIYDNVN SCLLSEVEIK GTKHGSGSTY INGFTLSVNH ALAWLEASKD
VTVIDLLYGW PLYQFHCWYE VTCVQCSLDG VYAFCGQYLN NTTIFHLGSG EKICTVTSEF
SGGFVKFLLI LDTAQEMVMV DSEGSLSVWN TEDISNPQLT EDFDCRKEDS EVVSIELSED
QSAILICKAL SIELLDTGMW KVAEKFRARH NERFVSAVLS KNGDCIIATM ENTPAVFFWR
RDTGQCLASL QESSGTIVKL VKSSHHNMLL SLSTSGVLSI WDIDIITAMS NIDKTGKPIQ
SLVLPARGEI IYSLDGSDCV HKWNFSSGFI EAVFKHEGIV EHCVLTSTGD LMVTSDDKSS
QYVWHTSSGE NLFRINGQRI SQLLITHNDQ FVVSLCEENA SRVWRLATGH RVCNILTTLQ
NAFITSANTF VVGMTKSKVL AVSLWTGSIT KKFCCEDGIT IVNFKLIPDC PDVIVFITSA
ETVNLWSLTD EVICRRVQLP SNFLKNLEDF EISPNGKLGI ISRGDENINV LDLHSGKLRV
VHASGVIWRQ RLSRDGRYLV YICFRNGEEE EENDAISSLI VMRLADGKNI GACSLYKTPT
FLALSQRHLN IIVGFDDGSI GIYTVVDRVD AALKIKIATS NSRQIFNNAT QTSRPKSNSY
SFKVSVDCLW RESTEVFARD SPITVSDSSE SNEATPSKKH NSCYDRVCAA LESRSHSYTP
DN
