NXF2_CAEEL
ID NXF2_CAEEL Reviewed; 405 AA.
AC Q9XVS8;
DT 15-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Nuclear RNA export factor 2;
GN Name=nxf-2 {ECO:0000312|WormBase:C15H11.6};
GN Synonyms=nfx-2 {ECO:0000312|WormBase:C15H11.6};
GN ORFNames=C15H11.6 {ECO:0000312|WormBase:C15H11.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=11529502; DOI=10.1007/pl00000924;
RA Izaurralde E.;
RT "A conserved family of nuclear export receptors mediates the exit of
RT messenger RNA to the cytoplasm.";
RL Cell. Mol. Life Sci. 58:1105-1112(2001).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23149939; DOI=10.1128/mcb.01298-12;
RA Shiimori M., Inoue K., Sakamoto H.;
RT "A specific set of exon junction complex subunits is required for the
RT nuclear retention of unspliced RNAs in Caenorhabditis elegans.";
RL Mol. Cell. Biol. 33:444-456(2013).
CC -!- FUNCTION: Involved in the export of cellular mRNA to the cytoplasm
CC (PubMed:23149939). Plays a role in the nuclear retention of unspliced
CC mRNAs (PubMed:23149939). {ECO:0000269|PubMed:23149939}.
CC -!- INTERACTION:
CC Q9XVS8; Q9U757: nxt-1; NbExp=5; IntAct=EBI-2007577, EBI-2006451;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11529502}. Note=Not
CC localized at the nuclear rim.
CC -!- DOMAIN: The RNA-binding domain is a non-canonical RNP-type domain.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in severe growth
CC retardation, lethality and reduces the accumulation of unspliced tra-2
CC in the cytoplasm of rnp-4/RBM8A RNAi mutants.
CC {ECO:0000269|PubMed:23149939}.
CC -!- SIMILARITY: Belongs to the NXF family. {ECO:0000305}.
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DR EMBL; BX284605; CAB02733.2; -; Genomic_DNA.
DR PIR; T19315; T19315.
DR RefSeq; NP_506568.2; NM_074167.6.
DR PDB; 3NV0; X-ray; 1.84 A; A=202-405.
DR PDBsum; 3NV0; -.
DR AlphaFoldDB; Q9XVS8; -.
DR SMR; Q9XVS8; -.
DR BioGRID; 44942; 5.
DR IntAct; Q9XVS8; 2.
DR STRING; 6239.C15H11.6.1; -.
DR EPD; Q9XVS8; -.
DR PaxDb; Q9XVS8; -.
DR PeptideAtlas; Q9XVS8; -.
DR EnsemblMetazoa; C15H11.6.1; C15H11.6.1; WBGene00003835.
DR GeneID; 179938; -.
DR KEGG; cel:CELE_C15H11.6; -.
DR UCSC; C15H11.6.1; c. elegans.
DR CTD; 179938; -.
DR WormBase; C15H11.6; CE36102; WBGene00003835; nxf-2.
DR eggNOG; KOG3763; Eukaryota.
DR GeneTree; ENSGT00390000007539; -.
DR HOGENOM; CLU_685590_0_0_1; -.
DR InParanoid; Q9XVS8; -.
DR OrthoDB; 1051093at2759; -.
DR PhylomeDB; Q9XVS8; -.
DR Reactome; R-CEL-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-CEL-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-CEL-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-CEL-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR PRO; PR:Q9XVS8; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00003835; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0016973; P:poly(A)+ mRNA export from nucleus; IBA:GO_Central.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR018222; Nuclear_transport_factor_2_euk.
DR InterPro; IPR030217; NXF_fam.
DR PANTHER; PTHR10662; PTHR10662; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR PROSITE; PS50177; NTF2_DOMAIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Leucine-rich repeat; mRNA transport; Nucleus;
KW Reference proteome; Repeat; RNA-binding; Transport.
FT CHAIN 1..405
FT /note="Nuclear RNA export factor 2"
FT /id="PRO_0000220537"
FT DOMAIN 26..94
FT /note="RRM"
FT REPEAT 97..118
FT /note="LRR 1"
FT REPEAT 123..144
FT /note="LRR 2"
FT DOMAIN 215..382
FT /note="NTF2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00137"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..33
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 210..227
FT /evidence="ECO:0007829|PDB:3NV0"
FT HELIX 232..235
FT /evidence="ECO:0007829|PDB:3NV0"
FT HELIX 236..242
FT /evidence="ECO:0007829|PDB:3NV0"
FT STRAND 245..247
FT /evidence="ECO:0007829|PDB:3NV0"
FT STRAND 249..255
FT /evidence="ECO:0007829|PDB:3NV0"
FT STRAND 260..262
FT /evidence="ECO:0007829|PDB:3NV0"
FT HELIX 269..277
FT /evidence="ECO:0007829|PDB:3NV0"
FT HELIX 282..284
FT /evidence="ECO:0007829|PDB:3NV0"
FT TURN 287..290
FT /evidence="ECO:0007829|PDB:3NV0"
FT HELIX 299..309
FT /evidence="ECO:0007829|PDB:3NV0"
FT STRAND 313..315
FT /evidence="ECO:0007829|PDB:3NV0"
FT HELIX 317..319
FT /evidence="ECO:0007829|PDB:3NV0"
FT STRAND 321..327
FT /evidence="ECO:0007829|PDB:3NV0"
FT STRAND 332..342
FT /evidence="ECO:0007829|PDB:3NV0"
FT HELIX 344..347
FT /evidence="ECO:0007829|PDB:3NV0"
FT STRAND 357..367
FT /evidence="ECO:0007829|PDB:3NV0"
FT STRAND 373..383
FT /evidence="ECO:0007829|PDB:3NV0"
FT HELIX 387..395
FT /evidence="ECO:0007829|PDB:3NV0"
SQ SEQUENCE 405 AA; 46202 MW; D15D1B3C602990DA CRC64;
MRGQNRRGYR NIEGRLSLSS HSSHSSPRQT HVTNLRPAEI KVVQDVVDGC YVATDDVLNL
SNFSKNTEFV ERDMLMCLTK TRVMSVVLQH IGYKYPRISG ISFSNNRLCH LDHLSSLSSI
SKFLKFLDLS HNQISSGEEL KKLGTIPVET VFFEGNPVCE KFVQCAEYAN FIQKTFPKCS
NLDGMEVEPK PDHNRIEQII PFRNGYYGSD EVRTLVEEFI ITYYKIYDGA DGQQTRKQLL
DAYDTNNSTF THTVVCLWDP IKFVMYPDSE SYRMYLRTSH NVLNQEYFAA NRASRISHGA
MDIVVALSRL PATIHLMDTF VVDVFLVSAT LLGFTLHGTF RDGPSAIKPE NTEEHDNYFT
RTFMVAPRGE GKVAIVSDQL FISSMSKRRG DQYRMLVETA TDIDQ
