ID   NXN_BOVIN               Reviewed;         435 AA.
AC   A6QLU8;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Nucleoredoxin;
DE            EC=1.8.1.8;
GN   Name=NXN;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal skin;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Functions as a redox-dependent negative regulator of the Wnt
CC       signaling pathway, possibly by preventing ubiquitination of DVL3 by the
CC       BCR(KLHL12) complex. May also function as a transcriptional regulator
CC       act as a regulator of protein phosphatase 2A (PP2A) (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[protein]-dithiol + NAD(+) = [protein]-disulfide + H(+) +
CC         NADH; Xref=Rhea:RHEA:18749, Rhea:RHEA-COMP:10593, Rhea:RHEA-
CC         COMP:10594, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950, ChEBI:CHEBI:50058,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.8.1.8;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[protein]-dithiol + NADP(+) = [protein]-disulfide + H(+) +
CC         NADPH; Xref=Rhea:RHEA:18753, Rhea:RHEA-COMP:10593, Rhea:RHEA-
CC         COMP:10594, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950, ChEBI:CHEBI:50058,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.8.1.8;
CC   -!- SUBUNIT: Associates with the phosphatase 2A holoenzyme. Interacts with
CC       PPP2CA; the interaction is direct. Interacts with DVL1 (via PDZ
CC       domain); the interaction is direct and regulated by oxidative stress
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:P97346}. Nucleus {ECO:0000250|UniProtKB:P97346}.
CC   -!- SIMILARITY: Belongs to the nucleoredoxin family. {ECO:0000305}.
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DR   EMBL; BC148092; AAI48093.1; -; mRNA.
DR   RefSeq; NP_001095606.1; NM_001102136.1.
DR   AlphaFoldDB; A6QLU8; -.
DR   STRING; 9913.ENSBTAP00000001140; -.
DR   PaxDb; A6QLU8; -.
DR   PRIDE; A6QLU8; -.
DR   Ensembl; ENSBTAT00000001140; ENSBTAP00000001140; ENSBTAG00000000855.
DR   GeneID; 531367; -.
DR   KEGG; bta:531367; -.
DR   CTD; 64359; -.
DR   VEuPathDB; HostDB:ENSBTAG00000000855; -.
DR   VGNC; VGNC:32376; NXN.
DR   eggNOG; KOG2501; Eukaryota.
DR   GeneTree; ENSGT00940000161894; -.
DR   HOGENOM; CLU_019626_2_1_1; -.
DR   InParanoid; A6QLU8; -.
DR   OMA; EMITRQG; -.
DR   OrthoDB; 1350271at2759; -.
DR   TreeFam; TF331873; -.
DR   Proteomes; UP000009136; Chromosome 19.
DR   Bgee; ENSBTAG00000000855; Expressed in vas deferens and 103 other tissues.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0004791; F:thioredoxin-disulfide reductase activity; IBA:GO_Central.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0072359; P:circulatory system development; ISS:UniProtKB.
DR   GO; GO:0001701; P:in utero embryonic development; IEA:Ensembl.
DR   GO; GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
DR   GO; GO:0030178; P:negative regulation of Wnt signaling pathway; ISS:UniProtKB.
DR   GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR   CDD; cd03071; PDI_b'_NRX; 1.
DR   CDD; cd03009; TryX_like_TryX_NRX; 1.
DR   InterPro; IPR041861; NRX_PDI_b.
DR   InterPro; IPR012336; Thioredoxin-like_fold.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   InterPro; IPR013766; Thioredoxin_domain.
DR   InterPro; IPR045870; TryX_NRX_thioredoxin_dom.
DR   Pfam; PF13905; Thioredoxin_8; 2.
DR   SUPFAM; SSF52833; SSF52833; 3.
DR   PROSITE; PS51352; THIOREDOXIN_2; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Cytoplasm; Developmental protein; Differentiation; NAD;
KW   Nucleus; Oxidoreductase; Reference proteome; Wnt signaling pathway.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q6DKJ4"
FT   CHAIN           2..435
FT                   /note="Nucleoredoxin"
FT                   /id="PRO_0000332932"
FT   DOMAIN          167..321
FT                   /note="Thioredoxin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT   MOD_RES         2
FT                   /note="N-acetylserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q6DKJ4"
SQ   SEQUENCE   435 AA;  48331 MW;  1A8582D78FBA2774 CRC64;
     MSGFLEELLG EKLVTGGGEE VDVHSLAARG ISLLGLYFGC SLSAPCAQLS ASLAAFYGRL
     RGDAAAGPGP GPGAGASAEP EPRRRLEIVF VSSDQDQRQW QDFVRDMPWL ALPYKEKHRK
     LKLWNKYRIS NIPSLIFLDA TSGKVVCRNG LLVIRDDPEG LEFPWGPKPF REVIAGPLLR
     SNGQSLESSS LEGSHVGVYF SAHWCPPCRS LTRVLVESYR KIKEAGQKFE IIFVSADRSE
     DSFKQYFSEM PWLAVPYTDE ARRSRLNRLY GIQGIPTLIV LDPQGEVITR QGRVEVLNDE
     DCRGFPWHPK PVLELSDSNA VQLNEGPCLV LFVDSEDDGE SEAAKQLIQP IAEKIIAKYK
     AKEEEAPLLF FVAGEDDMTD SLRDYTNLPE AAPLLTILDM SARAKYVMDV EEITPAIVEA
     FVNDFLAEKL KPEPI