ID   NXN_DANRE               Reviewed;         418 AA.
AC   Q503L9;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Nucleoredoxin;
DE            EC=1.8.1.8;
GN   Name=nxn; ORFNames=zgc:110449;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Olfactory epithelium;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Functions as a redox-dependent negative regulator of the Wnt
CC       signaling pathway. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[protein]-dithiol + NAD(+) = [protein]-disulfide + H(+) +
CC         NADH; Xref=Rhea:RHEA:18749, Rhea:RHEA-COMP:10593, Rhea:RHEA-
CC         COMP:10594, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950, ChEBI:CHEBI:50058,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.8.1.8;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[protein]-dithiol + NADP(+) = [protein]-disulfide + H(+) +
CC         NADPH; Xref=Rhea:RHEA:18753, Rhea:RHEA-COMP:10593, Rhea:RHEA-
CC         COMP:10594, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950, ChEBI:CHEBI:50058,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.8.1.8;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:P97346}. Nucleus {ECO:0000250|UniProtKB:P97346}.
CC   -!- SIMILARITY: Belongs to the nucleoredoxin family. {ECO:0000305}.
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DR   EMBL; BC095273; AAH95273.1; -; mRNA.
DR   RefSeq; NP_001018431.1; NM_001020595.1.
DR   AlphaFoldDB; Q503L9; -.
DR   STRING; 7955.ENSDARP00000037350; -.
DR   PaxDb; Q503L9; -.
DR   GeneID; 553621; -.
DR   KEGG; dre:553621; -.
DR   CTD; 64359; -.
DR   ZFIN; ZDB-GENE-050522-75; nxn.
DR   eggNOG; KOG2501; Eukaryota.
DR   InParanoid; Q503L9; -.
DR   OrthoDB; 1350271at2759; -.
DR   PhylomeDB; Q503L9; -.
DR   PRO; PR:Q503L9; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0004791; F:thioredoxin-disulfide reductase activity; IBA:GO_Central.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0072359; P:circulatory system development; ISS:UniProtKB.
DR   GO; GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
DR   GO; GO:0030178; P:negative regulation of Wnt signaling pathway; ISS:UniProtKB.
DR   GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR   CDD; cd03009; TryX_like_TryX_NRX; 1.
DR   InterPro; IPR012336; Thioredoxin-like_fold.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   InterPro; IPR013766; Thioredoxin_domain.
DR   InterPro; IPR045870; TryX_NRX_thioredoxin_dom.
DR   Pfam; PF13905; Thioredoxin_8; 2.
DR   SUPFAM; SSF52833; SSF52833; 3.
DR   PROSITE; PS51352; THIOREDOXIN_2; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Developmental protein; Differentiation; NAD; Nucleus;
KW   Oxidoreductase; Reference proteome; Wnt signaling pathway.
FT   CHAIN           1..418
FT                   /note="Nucleoredoxin"
FT                   /id="PRO_0000332935"
FT   DOMAIN          109..309
FT                   /note="Thioredoxin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
SQ   SEQUENCE   418 AA;  47410 MW;  42EA5F582152A88D CRC64;
     MSEFLVNLLG ERLVNGEKAE VDVQALGSRL SLLGLYFGCS LNGPCKQFNA SLTEFYSKFK
     KSSEHKDKLE IVFISSDQDQ KQWQDFLQEM QWPALPFKDR HKKMKLWNKY KVTSIPSLVF
     IDAATGKVVC RNGLLVVRDD PKGLEFPWGP KPFAEVVSGP LLRNNRQTTD STALEGSYVG
     VYFSAHWCPP CRSLTRVLVE SYRKVKETGQ KFEIVFVSAD RSEESFTQYF SEMPWLAVPY
     SDEARRSRLN RLYGIQGIPT LILLDTEGHM ITRQGRVEIL NDPDCGLFPW HPRPVLELSE
     SNAVQLHEGP CLVLFVDAEE EGELDPAKEL IQPIAEKIMA KYKAKEEETP LLFFVAGEDD
     MSDSLRDYTN LPEAAPLLTI LDMSARAKYV KDVEEITPAV VEQFVSGFLA EKLKPEPI