ID   NXS_SOLTU               Reviewed;         498 AA.
AC   Q9M424;
DT   13-OCT-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Neoxanthin synthase, chloroplastic;
DE            EC=5.3.99.9;
DE   Flags: Precursor;
GN   Name=NXS;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, AND SUBCELLULAR LOCATION.
RC   STRAIN=cv. Desiree; TISSUE=Tuber;
RX   PubMed=11094161; DOI=10.1016/s0014-5793(00)02193-1;
RA   Al-Babili S., Hugueney P., Schledz M., Welsch R., Frohnmeyer H., Laule O.,
RA   Beyer P.;
RT   "Identification of a novel gene coding for neoxanthin synthase from Solanum
RT   tuberosum.";
RL   FEBS Lett. 485:168-172(2000).
CC   -!- FUNCTION: Involved in the synthesis of neoxanthin, the last product of
CC       carotenoid synthesis and a precursor of abscisic acid.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-violaxanthin = all-trans-neoxanthin;
CC         Xref=Rhea:RHEA:10128, ChEBI:CHEBI:32446, ChEBI:CHEBI:35288;
CC         EC=5.3.99.9; Evidence={ECO:0000269|PubMed:11094161};
CC   -!- PATHWAY: Carotenoid biosynthesis; neoxanthin biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000269|PubMed:11094161}.
CC   -!- SIMILARITY: Belongs to the lycopene cyclase family. {ECO:0000305}.
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DR   EMBL; AJ272136; CAB92977.1; -; mRNA.
DR   RefSeq; NP_001305601.1; NM_001318672.1.
DR   AlphaFoldDB; Q9M424; -.
DR   SMR; Q9M424; -.
DR   STRING; 4113.PGSC0003DMT400015066; -.
DR   GeneID; 102601215; -.
DR   KEGG; sot:102601215; -.
DR   eggNOG; ENOG502QQD7; Eukaryota.
DR   InParanoid; Q9M424; -.
DR   BioCyc; MetaCyc:MON-16625; -.
DR   BRENDA; 5.3.99.9; 5757.
DR   UniPathway; UPA00388; -.
DR   Proteomes; UP000011115; Unassembled WGS sequence.
DR   ExpressionAtlas; Q9M424; baseline.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0009536; C:plastid; IDA:UniProtKB.
DR   GO; GO:0034020; F:neoxanthin synthase activity; IDA:UniProtKB.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0009688; P:abscisic acid biosynthetic process; IC:UniProtKB.
DR   GO; GO:0016120; P:carotene biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006744; P:ubiquinone biosynthetic process; IBA:GO_Central.
DR   GO; GO:0016123; P:xanthophyll biosynthetic process; IDA:UniProtKB.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR010108; Lycopene_cyclase_b/e.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01790; carotene-cycl; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Isomerase; Plastid; Reference proteome; Transit peptide.
FT   TRANSIT         1..33
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           34..498
FT                   /note="Neoxanthin synthase, chloroplastic"
FT                   /id="PRO_0000386524"
FT   REGION          16..38
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        16..34
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         84..112
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   498 AA;  56357 MW;  0CAB552D958C6F26 CRC64;
     METLLKPLTS LLLSSPTPHR SIFQQNPPSL NPTTKKKSRK CHFRNESSKL FCSFLDLAPI
     SKPESFDVNI SLVDPNSGRA QFDVIIIGAG PAGLRLAEHV SKYGIKVCCV DPSPLSMWPN
     NYGVWVDEFE NLGLEDCLDH KWPMTCVHIN DHKTKYLGRP YGRVSRKKLK LRLLNSCVEN
     RVKFYKAKVW KVEHEEFESS IVCDDGKKIR GSLVVDASGF ASDFIEYDKP RNHGYQIAHG
     VLVEVDNHPF DLDKMVLMDW RDSHLGNEPY LRVNNAKEPT FLYAMPFDRN LVFLEETSLV
     SRPVLSYMEV KRRMVARLRH LGIKVRSVIE EEKCVIPMGG PLPRIPQNVM AIGGNSGIVH
     PSTGYMVARS MALAPVLAEA IVKGLGSTRM IRGSQLYHRV WNGLWPLDRR CIGECYSFGM
     ETLLKLDLKG TRRLFDAFFD LDPKYWQGFL SSRLSVKELA ILSLCLFGHG SNLTRLDIVT
     KCPVPLVRLI GNLAIESL